HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, NCI-H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human FoxC1 Monoclonal Antibody (Catalog # MAB6329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6329-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89025-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05017-20ul | |||
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1268-img0015.jpg)
![Flow Cytometry: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody at 2 ug/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Flow-Cytometry-NB100-1268-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Breast Cancer subtype specificity of the EZH2/FOXC1 metastatic axis. a Representative images of formalin fixed paraffin-embedded tissue samples from human Luminal B tumours stained for FOXC1 & EZH2. Scale bar is 100 μm. b Significant negative correlation between FOXC1 transcript levels & EZH2 protein levels (Total intensity) quantified by immunofluorescence in human Luminal B patient samples (r = −0.52, p = 0.02). Eight different fields taken with a ×20 objective were quantified per sample. c Kaplan–Meier survival curve of relapse-free survival (RFS) of 1142 luminal B tumours with high or low FOXC1 (log rank p = 0.001). d Correlation between FOXC1 mRNA levels & the FOXC1 Signature in a cohort of 385 Luminal B patients from publicly available TCGA Breast Cancer data set. e Kaplan–Meier analysis of relapse-free survival of 1142 Luminal B tumours with high or low FOXC1 gene signature expression (log rank p = 0.0044). RFS relapse-free survival Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29959321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1268_goat-polyclonal-foxc1-antibody-310202415541964.jpg)
![Western Blot: FoxC1 Antibody [NB100-1268] - Ectopic expression of Foxc1 alters invasion but not proliferation. a Transwell invasion assay of PyVmT cells expressing exogenous Foxc1 or GFP. Experiments were performed three times, & results are displayed relative to untreated parental cell. *p < 0.05, Student’s two tailed t-tests. b CyQUANT assay measuring cellular proliferation of PyVmT cells expressing exogenous GFP or Foxc1. R.F.U relative fluorescence units. Data were normalized to the fluorescence values at time = 0. c Enumeration of lung lesions following the injection of PyVmT cells expressing exogenous Foxc1 or GFP in the tail vein of athymic nude mice. *p < 0.05, Student’s two tailed t-tests. Scale bar is 5 mm. d Left: Representative immunoblot showing Foxc1 expression in cells stably transduced with a control shRNA (shLacZ) or two independent shRNAs targeting Foxc1 & treated with DMSO or GSK-126 (2 μM for 72 h). alpha -Tubulin was used as a loading control. Right: Transwell invasion assay of PyVmT cells infected with shRNA targeting LacZ (control) or two different sequences targeting Foxc1. Cells were pre-treated with DMSO or GSK-126 (2 μM) for 72 h & assayed for their capacity to invade through Matrigel. All assays were performed in triplicate. *p < 0.05, Student’s two tailed t-tests Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29959321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1268_goat-polyclonal-foxc1-antibody-3102024168211.jpg)
![Immunohistochemistry: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32347) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32347_rabbit-foxc1-mab-psh03-89-29820241420930.jpg "Immunohistochemistry: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32347) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Immunocytochemistry analysis of HeLa cells labeling FoxC1 with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32347_rabbit-foxc1-mab-psh03-89-2920241114841.jpg)
![Western blot analysis on different lysates with NBP3-32347 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: C2C12 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32347 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32347_-western-blot-639204590071063562.jpg)
![Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NBP1-89025] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89025-img0009.jpg "Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NBP1-89025] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NBP1-89025] - Mouse embryonic brain section was stained with anti-FOXC1 antibody.Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89025-img0004.jpg)
![Immunohistochemistry:[NBP3-30290]-Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1: 200. Detection: DAB. Counterstain: hematoxylin.](http://images.novusbio.com/fullsize/nbp3-30290_rabbit-foxc1-pab-231202511585577.jpg "Immunohistochemistry:[NBP3-30290]-Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1: 200. Detection: DAB. Counterstain: hematoxylin.")
![Immunohistochemistry;FoxC1 Antibody[NBP3-29487]Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1:1,000 . Detection: DAB. Counterstain: hematoxylin .](http://images.novusbio.com/fullsize/nbp3-29488_rabbit-foxc1-pab-78202410153523.jpg "Immunohistochemistry;FoxC1 Antibody[NBP3-29487]Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1:1,000 . Detection: DAB. Counterstain: hematoxylin .")
![Immunoprecipitation:FoxC1 AntibodyNBP3-29488] -Detection of human FOXC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-FOXC1 antibody NBP3-29488 used for IP at 6 µg/mg lysate. FOXC1 was also immunoprecipitated by rabbit anti-FOXC1., which recognizes a downstream epitope. For blotting immunoprecipitated FOXC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29488_rabbit-foxc1-pab-301220241633621.jpg)
![Western Blot: FoxC1 Antibody [NBP3-05017] - Analysis of extracts of various cell lines, using FoxC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FoxC1-Antibody-Western-Blot-NBP3-05017-img0001.jpg "Western Blot: FoxC1 Antibody [NBP3-05017] - Analysis of extracts of various cell lines, using FoxC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: FOXC1 Overexpression Lysate (Adult Normal) [NBL1-10801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXC1.](http://images.novusbio.com/fullsize/FOXC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10801-img0002.jpg "Western Blot: FOXC1 Overexpression Lysate (Adult Normal) [NBL1-10801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXC1.")
![Western Blot: FoxC1 Antibody (SR2414) [NBP3-27713] - Western blot analysis of FoxC1 in 3T3 lysates](http://images.novusbio.com/fullsize/nbp3-27713_rabbit-foxc1-mab-sr2414-75202410133432.jpg "Western Blot: FoxC1 Antibody (SR2414) [NBP3-27713] - Western blot analysis of FoxC1 in 3T3 lysates")
