| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92968-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70464 | |||
HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4567) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4567-SP | |||
![Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NB100-93388-img0003.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93388-img0005.jpg)
![Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of paraffin embedded human tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunohistochemistry-Paraffin-NB100-93388-img0002.jpg)
![Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Flow-Cytometry-NB100-93388-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NB100-93388-img0004.jpg)
![Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NBP2-92968-img0005.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Knockout-Validated-NBP2-92968-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968] - Immunofluorescence analysis of HeLa cells using [KO Validated] Casein Kinase 1 epsilon Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92968_rabbit-polyclonal-casein-kinase-1-epsilon-antibody-422025143182.jpg%3Ft=1709606037)
![Western Blot: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Casein Kinase 1 epsilon.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-5D4-Western-Blot-NBP2-46377-img0005.jpg "Western Blot: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Casein Kinase 1 epsilon.")
![Immunohistochemistry: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-5D4-Immunohistochemistry-NBP2-46377-img0004.jpg)
![Immunohistochemistry: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-5D4-Immunohistochemistry-NBP2-46377-img0001.jpg)
![Immunohistochemistry: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of Human endometrium tissue . (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-5D4-Immunohistochemistry-NBP2-46377-img0002.jpg)
![Immunohistochemistry: Casein Kinase 1 epsilon Antibody (5D4) [NBP2-46377] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-5D4-Immunohistochemistry-NBP2-46377-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody (2E1) [H00001454-M07] - Analysis of monoclonal antibody to CSNK1E on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-2E1-Immunocytochemistry-Immunofluorescence-H00001454-M07-img0001.jpg "Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody (2E1) [H00001454-M07] - Analysis of monoclonal antibody to CSNK1E on HeLa cell. Antibody concentration 10 ug/ml")
![Proximity Ligation Assay: Casein Kinase 1 epsilon Antibody (2E1) [H00001454-M07] - Analysis of protein-protein interactions between TP53 and CSNK1E. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-2E1-Proximity-Ligation-Assay-H00001454-M07-img0002.jpg)
![Western Blot: CSNK1E Overexpression Lysate (Adult Normal) [NBL1-09532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSNK1E.](http://images.novusbio.com/fullsize/CSNK1E-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09532-img0002.jpg "Western Blot: CSNK1E Overexpression Lysate (Adult Normal) [NBL1-09532] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSNK1E.")
![Western Blot: CSNK1E Overexpression Lysate (Adult Normal) [NBL1-09531] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSNK1E.](http://images.novusbio.com/fullsize/CSNK1E-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09531-img0002.jpg "Western Blot: CSNK1E Overexpression Lysate (Adult Normal) [NBL1-09531] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSNK1E.")
