Products Related to Gene-ID: 140885

HAF018). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP alpha /CD172a
Monoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

NL007) and counterstained with DAPI. Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI. Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

4670-CD) binds with an ED₅₀ of 0.04-0.24 µg/mL." class="big_lightbox"> 4670-CD) binds with an ED₅₀ of 0.04-0.24 µg/mL." alt="When Recombinant Human SIRP alpha/CD172a (Catalog # 9378-SA) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) binds with an ED₅₀ of 0.04-0.24 µg/mL." class="big_thumb" />

AVI4670) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human SIRP alpha His protein (Catalog # 9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_lightbox"> AVI4670) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human SIRP alpha His protein (Catalog # 9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_thumb" />

HAF016). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, A549 human lung carcinoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SIRP alpha /CD172a
Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4546) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF018). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

7307-SA) supports the adhesion in mouse red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat SIRPa/CD172a (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307). The ND₅₀ is typically 0.04-0.16 µg/mL." class="big_lightbox"> 7307-SA) supports the adhesion in mouse red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat SIRPa/CD172a (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307). The ND₅₀ is typically 0.04-0.16 µg/mL." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

HAF016). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse/Rat SIRPa/CD172a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7307) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

4670-CD) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRPa/CD172a Fc Chimera Avi-tag (Catalog # AVI4546) binds with an ED₅₀ of 20-180 ng/mL." class="big_lightbox"> 4670-CD) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRPa/CD172a Fc Chimera Avi-tag (Catalog # AVI4546) binds with an ED₅₀ of 20-180 ng/mL." alt="When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human SIRPa/CD172a Fc Chimera Avi-tag (Catalog # AVI4546) binds with an ED₅₀ of 20-180 ng/mL." class="big_thumb" />

2 μg/lane of Biotinylated Recombinant Human SIRP alpha /CD172a Fc Chimera Avi-tag (Catalog # AVI4546) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-96 kDa and 170- 195 kDa, respectively.