| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5127-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5127-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86181-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6910-SP | |||
![Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Western-Blot-H00001399-M03-img0008.jpg "Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysatE (33.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-CRKL mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0006.jpg)
![Western Blot: CrkL Antibody (4B5) [H00001399-M03] - Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi or non-transfected control. Blot probed with H00001399-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Western-Blot-H00001399-M03-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of monoclonal antibody to CRKL on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00001399-M03-img0005.jpg)
![ELISA: CrkL Antibody (4B5) [H00001399-M03] - Detection limit for recombinant GST tagged CRKL is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-ELISA-H00001399-M03-img0003.jpg)
![Proximity Ligation Assay: CrkL Antibody (4B5) [H00001399-M03] - Analysis of protein-protein interactions between HCK and CRKL. Mahlavu cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction co](http://images.novusbio.com/fullsize/CrkL-Antibody-4B5-Proximity-Ligation-Assay-H00001399-M03-img0007.jpg)
![Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunohistochemistry-Paraffin-NB300-884-img0001.jpg "Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: CrkL Antibody [NB300-884] - (0.1ug/ml) staining of K562 (A) and (1ug/ml) Daudi (B) and NIH3T3 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918333.jpg)
![Flow Cytometry: CrkL Antibody [NB300-884] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-1652023918337.jpg)
![Immunocytochemistry/Immunofluorescence: CrkL Antibody [NB300-884] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb300-884_goat-polyclonal-crkl-antibody-16520239183315.jpg)
![Western Blot: CrkL Antibody (JE50-21) [NBP3-32222] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32222) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: A431 cell lysate (20 ug/Lane) Lane 3: HT-29 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32222) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-278202416285269.jpg "Western Blot: CrkL Antibody (JE50-21) [NBP3-32222] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32222) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: A431 cell lysate (20 ug/Lane) Lane 3: HT-29 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: NIH/3T3 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32222) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CrkL Antibody (JE50-21) [NBP3-32222] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (NBP3-32222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-298202414333684.jpg)
![Flow Cytometry: CrkL Antibody (JE50-21) [NBP3-32222] - Flow cytometric analysis of A431 cells labeling CrkL. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32222, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32222_rabbit-crkl-mab-je50-21-392024884662.jpg)
![Western Blot: CrkL Antibody (GT466) [NBP3-13625] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (NBP3-13625) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Western-Blot-NBP3-13625-img0001.jpg "Western Blot: CrkL Antibody (GT466) [NBP3-13625] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody [GT466] (NBP3-13625) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Immunohistochemistry-Paraffin-NBP3-13625-img0003.jpg)
![Immunohistochemistry-Paraffin: CrkL Antibody (GT466) [NBP3-13625] - CrkL antibody [GT466] detects CrkL protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CrkL stained by CrkL antibody [GT466] (NBP3-13625) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CrkL-Antibody-GT466-Immunohistochemistry-Paraffin-NBP3-13625-img0002.jpg)
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0003.jpg "Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0001.jpg)
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-13318] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CrkL (phospho Tyr207) antibody (NBP3-13318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CrkL-[p-Tyr207]-Antibody-Western-Blot-NBP3-13318-img0002.jpg)
![Western Blot: CrkL Antibody [NBP2-55130] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/CrkL-Antibody-Western-Blot-NBP2-55130-img0003.jpg "Western Blot: CrkL Antibody [NBP2-55130] - Analysis in human cell line K562.")
![Immunocytochemistry/Immunofluorescence: CrkL Antibody [NBP2-55130] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CrkL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55130-img0001.jpg)
![Western Blot: CrkL Antibody [NBP3-23256] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23256_rabbit-crkl-pab-1992023191547.jpg "Western Blot: CrkL Antibody [NBP3-23256] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).")
![Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23257_rabbit-crkl-p-tyr207-pab-199202312403715.jpg "Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).")