| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41187-0.025mg | |||
MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Claudin-4 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB42191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB42191-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21843-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26653-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26654-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26655-50ul | |||
![Western Blot: Claudin-4 Antibody [NBP2-41187] - Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: CLAUDIN4, NBP2-41187 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-4-Antibody-Western-Blot-NBP2-41187-img0005.jpg "Western Blot: Claudin-4 Antibody [NBP2-41187] - Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: CLAUDIN4, NBP2-41187 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: Claudin-4 Antibody [NBP2-41187] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: CLAUDIN4, NBP2-41187 (1 ug/mL), CLAUDIN4 from Company B (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-4-Antibody-Western-Blot-NBP2-41187-img0003.jpg)
![Western Blot: Claudin-4 Antibody [NBP2-41187] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: CLAUDIN4, NBP2-41187 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-4-Antibody-Western-Blot-NBP2-41187-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Claudin-4 Antibody - BSA Free [NBP2-41187] - Validation in Human Colon Tissue Lysate. Loading: 15 ug of lysates per lane.Antibodies: Claudin-4, (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41187_rabbit-polyclonal-claudin-4-antibody-271120241932651.jpg)
![ELISA: Human Claudin-4 ELISA Kit (Colorimetric) [NBP2-75330] - Samples were spiked with high concentrations of Human Claudin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75330_human-claudin-4-elisa-kit-colorimetric-132202416175628.jpg "ELISA: Human Claudin-4 ELISA Kit (Colorimetric) [NBP2-75330] - Samples were spiked with high concentrations of Human Claudin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Claudin-4 ELISA Kit (Colorimetric) [NBP2-75330] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75330_human-claudin-4-elisa-kit-colorimetric-43202498355.jpg)
![Immunoprecipitation: Claudin-4 Antibody (4A11) [H00001364-M02] - Analysis of CLDN4 transfected lysate using anti-CLDN4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN4 MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/Claudin-4-Antibody-4A11-Immunoprecipitation-H00001364-M02-img0002.jpg "Immunoprecipitation: Claudin-4 Antibody (4A11) [H00001364-M02] - Analysis of CLDN4 transfected lysate using anti-CLDN4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN4 MaxPab mouse polyclonal antibody.")
![Sandwich ELISA: Claudin-4 Antibody (4A11) [H00001364-M02] - Detection limit for recombinant GST tagged CLDN4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-4-Antibody-4A11-Sandwich-ELISA-H00001364-M02-img0003.jpg)
![Western Blot: Claudin-4 Antibody (SR1284) [NBP3-21843] - Western blot analysis using NBP3-21843 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21843_rabbit-claudin-4-mab-sr1284-57202315545114.jpg "Western Blot: Claudin-4 Antibody (SR1284) [NBP3-21843] - Western blot analysis using NBP3-21843 in MCF7 cell lysate.")
![Western Blot: Claudin 4 Overexpression Lysate (Adult Normal) [NBL1-09246] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 4.](http://images.novusbio.com/fullsize/Claudin-4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09246-img0002.jpg "Western Blot: Claudin 4 Overexpression Lysate (Adult Normal) [NBL1-09246] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 4.")
![Flow Cytometry: Claudin-4 Antibody (10F4) [NBP3-26653] - Overlay Peak curve showing MCF7 cells stained with Claudin-4 Antibody (10F4) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26653_mouse-claudin-4-mab-10f4-282202414312729.jpg "Flow Cytometry: Claudin-4 Antibody (10F4) [NBP3-26653] - Overlay Peak curve showing MCF7 cells stained with Claudin-4 Antibody (10F4) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
![ELISA: Claudin-4 Antibody (8G7) [NBP3-26654] -The Binding Activity of Human CLDN4 with Anti-CLDN4 Recombinant Antibody<br />Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN4 at 5 μg/mL can bind Anti-CLDN4 recombinant antibody, the EC<sub>50</sub> is 29.56-50.75 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26654_mouse-claudin-4-mab-6e10-29120251248503.jpg "ELISA: Claudin-4 Antibody (8G7) [NBP3-26654] -The Binding Activity of Human CLDN4 with Anti-CLDN4 Recombinant Antibody<br />Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN4 at 5 μg/mL can bind Anti-CLDN4 recombinant antibody, the EC<sub>50</sub> is 29.56-50.75 ng/mL.")
