CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="VAMP-5 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Sheep Anti-Human VAMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7366-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85913-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50357-0.025ml | |||
![Immunohistochemistry-Paraffin: VAMP-5 Antibody [NBP1-85913] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VAMP-5-Antibody-Immunohistochemistry-Paraffin-NBP1-85913-img0002.jpg "Immunohistochemistry-Paraffin: VAMP-5 Antibody [NBP1-85913] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.")
![Western Blot: VAMP-5 Antibody (3E5) [NBP3-13515] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-5 antibody [3E5] (NBP3-13515) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13515_mouse-vamp-5-mab-3e5-9102024209140.jpg "Western Blot: VAMP-5 Antibody (3E5) [NBP3-13515] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-5 antibody [3E5] (NBP3-13515) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![ICC/IF analysis of pLEGFP-C1-hV5p transfected HeLa cells using NBP3-13515 VAMP5 antibody [3E5].<br>Red : Primary antibody](http://images.novusbio.com/fullsize/nbp3-13515_mouse-vamp-5-mab-3e5-immunocytochemistry-immunofluorescence-261120251833103.jpg)
![ICC/IF analysis of transfected NRK cells (V5V4-GFP) using NBP3-13515 VAMP5 antibody [3E5].<br>Red : Primary antibody](http://images.novusbio.com/fullsize/nbp3-13515_mouse-vamp-5-mab-3e5-immunocytochemistry-immunofluorescence-2611202518454011.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-5 Antibody (3E5) [NBP2-50357] - Staining of transfected NRK cells.](http://images.novusbio.com/fullsize/VAMP-5-Antibody-3E5-Immunocytochemistry-Immunofluorescence-NBP2-50357-img0001.jpg "Immunocytochemistry/Immunofluorescence: VAMP-5 Antibody (3E5) [NBP2-50357] - Staining of transfected NRK cells.")
![Immunocytochemistry/Immunofluorescence: VAMP-5 Antibody (3E5) [NBP2-50357] - Immunofluorescence on transfected HeLa cells (pLEGFP-C1-hV5p) using anti-Vamp5 [3E5].](http://images.novusbio.com/fullsize/VAMP-5-Antibody-3E5-Immunocytochemistry-Immunofluorescence-NBP2-50357-img0002.jpg)
![Western Blot: VAMP5 Overexpression Lysate (Adult Normal) [NBL1-17690] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VAMP5.](http://images.novusbio.com/fullsize/VAMP5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17690-img0002.jpg "Western Blot: VAMP5 Overexpression Lysate (Adult Normal) [NBL1-17690] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VAMP5.")
![Sandwich ELISA: VAMP-5 Antibody (3D4) [H00010791-M05] - Detection limit for recombinant GST tagged VAMP5 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VAMP-5-Antibody-3D4-Sandwich-ELISA-H00010791-M05-img0001.jpg "Sandwich ELISA: VAMP-5 Antibody (3D4) [H00010791-M05] - Detection limit for recombinant GST tagged VAMP5 is 0.1 ng/ml as a capture antibody.")
![SDS-Page: Recombinant Human VAMP-5 His Protein [NBC1-22935] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human VAMP-5 His Protein-SDS-Page-NBC1-22935-img0002.jpg "SDS-Page: Recombinant Human VAMP-5 His Protein [NBC1-22935] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
