CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Neuregulin-3/NRG3 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Mouse Neuregulin-3/NRG3 EGF-like Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF753) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF753-SP | |||
1131-ER) with anED50 of 5.00-50.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. Recombinant Human Neuregulin‑3/NRG3 His-tag Protein (Catalog # 11520-NR) binds to Recombinant Human ErbB4/Her4 Fc Chimera Protein (1131-ER) with anED50 of 5.00-50.0 ng/mL." class="big_thumb" />
1131-ER) with a ED50 of 15.0-150 ng/mL." class="big_thumb" />
![ELISA: Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) [NBP2-76761] - Samples were spiked with high concentrations of Human Neuregulin-3/NRG3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76761_human-neuregulin-3-nrg3-elisa-kit-colorimetric-132202416143061.jpg "ELISA: Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) [NBP2-76761] - Samples were spiked with high concentrations of Human Neuregulin-3/NRG3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) [NBP2-76761] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76761_human-neuregulin-3-nrg3-elisa-kit-colorimetric-4320249173427.jpg)
![Western Blot: Neuregulin 3 Antibody [NBP1-59338] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Neuregulin-3-Antibody-Western-Blot-NBP1-59338-img0001.jpg "Western Blot: Neuregulin 3 Antibody [NBP1-59338] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: Neuregulin 3 Antibody [NBP1-59338] - Human Brain, cortex tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/Neuregulin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-59338-img0003.jpg)
![Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670] - Analysis of Neuregulin 3 in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neuregulin-3-NRG3-Antibody-Western-Blot-NB100-61670-img0002.jpg "Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670] - Analysis of Neuregulin 3 in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Neuregulin-3/NRG3 Antibody (6V9X1) [NBP3-15901] - Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3/NRG3) antibody (NBP3-15901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Neuregulin-3-NRG3-Antibody-6V9X1-Western-Blot-NBP3-15901-img0001.jpg "Western Blot: Neuregulin-3/NRG3 Antibody (6V9X1) [NBP3-15901] - Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3/NRG3) antibody (NBP3-15901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: Neuregulin-3/NRG3 Antibody (6V9X1) [NBP3-15901] - Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3/NRG3) antibody (NBP3-15901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Neuregulin-3-NRG3-Antibody-6V9X1-Western-Blot-NBP3-15901-img0002.jpg)
![Western Blot: Neuregulin-3/NRG3 Antibody (S02-9E5) [NBP3-19721] - Western blot detection of Neuregulin-3/NRG3 in K562, Rat Brain lysates using NBP3-19721 (1:1000 diluted). Predicted band size: 78kDa. Observed band size: 55kDa.](http://images.novusbio.com/fullsize/Neuregulin-3-NRG3-Antibody-S02-9E5-Western-Blot-NBP3-19721-img0001.jpg "Western Blot: Neuregulin-3/NRG3 Antibody (S02-9E5) [NBP3-19721] - Western blot detection of Neuregulin-3/NRG3 in K562, Rat Brain lysates using NBP3-19721 (1:1000 diluted). Predicted band size: 78kDa. Observed band size: 55kDa.")
