HAF016). A specific band was detected for Teneurin-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6324-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41315-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93336-0.02ml | |||
![Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Western-Blot-NBP2-41315-img0001.jpg "Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.")
![Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] - HINT1 exhibits isopeptidase activity. (A) HINT1 interactions with the proteins used in the study. (I) HINT1 but not its paralogs forms stable complexes with RGSZ2 proteins. (II) ICD teneurin1 interacts with HINT1 but not with GST. (III, IV) HINT1 associations with RanGAP1 & SP100 were weak or not detected. (B) HINT1 exhibits SUMO protease activity on sumoylated RanGAP1, which is regulated by calcium & CaM. (I) HINT1 (2 μM) but not SENP2 (0.3 μM) requires calcium-activated CaM (6 μM) to exhibit sumoylase activity. (II) Removal of 10 mM MgCl2 from the sumoylation buffer greatly improves Ca2+-CaM-dependent HINT1 isopeptidase activity on sumoylated RanGAP1. (III, IV) HINT1 exhibits isopeptidase activity on sumoylated RanGAP1 in the presence of increasing concentrations of CaCl2 & of CaM. Details of immunoblot detection in “Materials & Methods” section & Supplementary Figures S5 & S6. GST, glutathione S-transferase; ICD, intracellular domain; RanGAP1, Ran GTPase-activating protein 1; RGSZ2, regulator of G protein signaling 17 (Z2); SENP, sentrin-specific protease. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31088288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-31020241611353.jpg)
![Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunofluorescence of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-271120241932664.jpg)
![Immunohistochemistry: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunohistochemistry of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-2711202419353882.jpg)
![ELISA: Teneurin-1 Antibody (3G8) [H00010178-M01] - Detection limit for recombinant GST tagged ODZ1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-3G8-ELISA-H00010178-M01-img0001.jpg "ELISA: Teneurin-1 Antibody (3G8) [H00010178-M01] - Detection limit for recombinant GST tagged ODZ1 is approximately 1ng/ml as a capture antibody.")
![Sandwich ELISA: Teneurin-1 Antibody (3G8) [H00010178-M01] - Detection limit for recombinant GST tagged ODZ1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-3G8-Sandwich-ELISA-H00010178-M01-img0002.jpg)
![Western Blot: Teneurin-1 Antibody [NBP2-93336] - Analysis of extracts of various cell lines, using Teneurin-1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Western-Blot-NBP2-93336-img0004.jpg "Western Blot: Teneurin-1 Antibody [NBP2-93336] - Analysis of extracts of various cell lines, using Teneurin-1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Teneurin-1 Antibody [NBP2-93336] - Analysis of U-2 OS cells using Teneurin-1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93336-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Teneurin-1 Antibody [NBP2-93336] - Analysis of C6 cells using Teneurin-1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93336-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - Azide and BSA Free [NBP2-93336] - Immunofluorescence analysis of L929 cells using Teneurin-1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93336_rabbit-polyclonal-teneurin-1-antibody-42202514264487.jpg%3Ft=1709174617)
![Immunohistochemistry-Paraffin: Teneurin-1 Antibody [NBP2-52739] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-52739-img0001.jpg "Immunohistochemistry-Paraffin: Teneurin-1 Antibody [NBP2-52739] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: Teneurin-1 Antibody [NBP3-13409] - Teneurin-1 antibody detects Teneurin-1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. Teneurin-1 stained by Teneurin-1 antibody (NBP3-13409) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13409-img0001.jpg "Immunohistochemistry-Paraffin: Teneurin-1 Antibody [NBP3-13409] - Teneurin-1 antibody detects Teneurin-1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. Teneurin-1 stained by Teneurin-1 antibody (NBP3-13409) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
