| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-79857 | |||
HAF018). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1032-SP | |||
| Carrier Free | MAB1032-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88239-25ul | |||
HAF008). A specific band was detected for Cadherin-17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin-17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8524-SP | |||
HAF017). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1032) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1032-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88238-25ul | |||
HAF008). A specific band was detected for Cadherin-17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88237-25ul | |||
![Western Blot: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Western blot analysis of Cadherin-17 on different lysates with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/1,000 dilution.<br/><br/> Lane 1: Caco-2 cell lysate (20 ug/Lane)<br/> Lane 2: LoVo cell lysate (30 ug/Lane)<br/> Lane 3: PANC-1 cell lysate (negative) (30 ug/Lane)<br/><br/> Predicted band size: 92 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32535) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32535_rabbit-cadherin-17-mab-je60-34-278202416285262.jpg "Western Blot: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Western blot analysis of Cadherin-17 on different lysates with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/1,000 dilution.<br/><br/> Lane 1: Caco-2 cell lysate (20 ug/Lane)<br/> Lane 2: LoVo cell lysate (30 ug/Lane)<br/> Lane 3: PANC-1 cell lysate (negative) (30 ug/Lane)<br/><br/> Predicted band size: 92 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32535) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling Cadherin-17 with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32535, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32535_rabbit-cadherin-17-mab-je60-34-298202413585514.jpg)
![Immunohistochemistry: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32535) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32535_rabbit-cadherin-17-mab-je60-34-29820241435293.jpg)
![Immunocytochemistry/ Immunofluorescence: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Immunocytochemistry analysis of Caco-2 cells labeling Cadherin-17 with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cadherin-17 antibody (NBP3-32535) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32535_rabbit-cadherin-17-mab-je60-34-2920241023425.jpg)
![Flow Cytometry: Cadherin-17 Antibody (JE60-34) [NBP3-32535] - Flow cytometric analysis of Caco-2 cells labeling Cadherin-17.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32535, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32535_rabbit-cadherin-17-mab-je60-34-392024884624.jpg)
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody (CDH17/2618) [NBP2-79723] - Formalin-fixed, paraffin-embedded human Colon stained with Cadherin-17 Antibody (CDH17/2618).](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-CDH17-2618-Immunohistochemistry-Paraffin-NBP2-79723-img0002.jpg "Immunohistochemistry-Paraffin: Cadherin-17 Antibody (CDH17/2618) [NBP2-79723] - Formalin-fixed, paraffin-embedded human Colon stained with Cadherin-17 Antibody (CDH17/2618).")
![Flow Cytometry: Cadherin-17 Antibody (CDH17/2618) [NBP2-79723] - Flow Cytometric Analysis of MCF-7 cells using Cadherin-17 Antibody (CDH17/2618)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-CDH17-2618-Flow-Cytometry-NBP2-79723-img0001.jpg)
![Protein Array: Cadherin-17 Antibody (CDH17/2618) [NBP2-79723] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cadherin-17 Antibody (CDH17/2618). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-CDH17-2618-Protein-Array-NBP2-79723-img0003.jpg)
NL007) and counterstained with DAPI (blue)." class="big_thumb" />
NL007) and counterstained with DAPI (blue)." class="big_thumb" />
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88239] - Analysis in human duodenum and liver tissues using NBP1-88239 antibody. Corresponding Cadherin-17 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Immunohistochemistry-Paraffin-NBP1-88239-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88239] - Analysis in human duodenum and liver tissues using NBP1-88239 antibody. Corresponding Cadherin-17 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88239] - Staining of human colon, duodenum, kidney and liver using Anti-Cadherin-17 antibody NBP1-88239 (A) shows similar protein distribution across tissues to independent antibody NBP1-88238 (B).](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Immunohistochemistry-Paraffin-NBP1-88239-img0019.jpg)
![Simple Western: Cadherin-17 Antibody [NBP1-88239] - Simple Western lane view shows a specific band for Cadherin-17 in 0.2 mg/ml of Colo205 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Simple-Western-NBP1-88239-img0004.jpg)
![Simple Western: Cadherin-17 Antibody [NBP1-88239] - Electropherogram image of the corresponding Simple Western lane view. Cadherin-17 antibody was used at 1:20 dilution on Colo205 lysate(s) respectively.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Simple-Western-NBP1-88239-img0005.jpg)
NL004) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for 
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for 
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Analysis in human duodenum and liver tissues using NBP1-88238 antibody. Corresponding Cadherin-17 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Immunohistochemistry-Paraffin-NBP1-88238-img0013.jpg)
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Immunohistochemistry-Paraffin-NBP1-88238-img0005.jpg)
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88238] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88238_rabbit-polyclonal-cadherin-17-antibody-immunohistochemistry-paraffin-213202495322..jpg)
NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for 
![Western Blot: Cadherin-17 Antibody (1H3) [H00001015-M01] - CDH17 monoclonal antibody (M01), clone 1H3. Analysis of CDH17 expression in human intestinal wall.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-1H3-Western-Blot-H00001015-M01-img0007.jpg "Western Blot: Cadherin-17 Antibody (1H3) [H00001015-M01] - CDH17 monoclonal antibody (M01), clone 1H3. Analysis of CDH17 expression in human intestinal wall.")
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody (1H3) [H00001015-M01] - Analysis of monoclonal antibody to CDH17 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-1H3-Immunohistochemistry-Paraffin-H00001015-M01-img0005.jpg)
![ELISA: Cadherin-17 Antibody (1H3) [H00001015-M01] - Detection limit for recombinant GST tagged CDH17 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-1H3-ELISA-H00001015-M01-img0008.jpg)
![Western Blot: Cadherin-17 Antibody [NBP1-88237] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Western-Blot-NBP1-88237-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cadherin-17 Antibody [NBP1-88237] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.")
![Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Analysis in human duodenum and liver tissues using NBP1-88237 antibody. Corresponding Cadherin-17 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cadherin-17-Antibody-Immunohistochemistry-Paraffin-NBP1-88237-img0018.jpg)
