HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysate of ZR-75 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # MAB861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF018). A specific band was detected for P-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog #
F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog #
F0101B). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
HAF018). A specific band was detected for P-Cadherin at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog #
MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for P-Cadherin at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog #
MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog #
F0102B). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">

MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog #
F0102B). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
861-PC) was serially diluted and captured by Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF861) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

861-PC) was serially diluted and captured by Mouse Anti-Human P‑Cadherin Monoclonal Antibody (Catalog # MAB861) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human P‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF861) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />