| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8910-SP | |||
| Carrier Free | FAB8910R-025 | |||
| Carrier Free | FAB8910V-100UG | |||
| Carrier Free | FAB8910U-100UG | |||
| Carrier Free | FAB8910T-100UG | |||
| Carrier Free | FAB8910S-100UG | |||
| Carrier Free | FAB8910N-100UG | |||
| Carrier Free | FAB8910G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-744-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50099-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Immunocytochemistry-Immunofluorescence-NBP2-54609-img0022.jpg "Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Surface Plasmon Resonance: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - A synthesized biotin-labeled 5-mC conjugate was immobilized on a sensorchip. Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control, while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 uL/min. Interaction assays involved injections of 2 different dilutions of the 5-mC monoclonal antibody over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 minute washing step with HBS-N buffer. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH 3). The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Ovalbumine-Surface-Plasmon-Resonance-NBP2-54609-img0015.jpg)
![Dot Blot: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - To demonstrate the specificity of the antibody against 5-mC, a Dot blot analysis was performed using hmC, mC and C controls. 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. The antibody was used at a dilution of 1:300. Figure shows a high specificity of the antibody for the methylated control.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Dot-Blot-NBP2-54609-img0018.jpg)
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - Analysis was performed on 1 ug fragmented human genomic DNA using 0.2 ug of the monoclonal antibody against 5-mC. The fragmented DNA was spiked with controls (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with primer sets specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. The figure shows the recovery expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Methylated-DNA-Immunoprecipitation-NBP2-54609-img0023.jpg)
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody diluted at 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0008.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody diluted at 1:200.")
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0002.jpg)
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse testis. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0004.jpg)
![Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Whole-Mount-NBP2-42814-img0005.jpg)
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Methylated-DNA-Immunoprecipitation-NBP2-42814-img0006.jpg)
![Dot Blot: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine .](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-4111-Dot-Blot-NBP2-42814-img0001.jpg)
![Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0006.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.](http://images.novusbio.com/fullsize/Cytosine-5-Methyl-Antibody-Immunocytochemistry-Immunofluorescence-NB100-744-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 to 5 were loaded with proteins (2 ug) from HFFs, hESCs, inhibitor treated HFFs and combined treated HFFs. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0005.jpg)
![ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Direct ELISA of HeLa cell genomic DNA using NBP2-61470. The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. NBP2-61470 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-61470_rabbit-monoclonal-5-methylcytosine-antibody-rm231-elisa-18920252337111.png "ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Direct ELISA of HeLa cell genomic DNA using NBP2-61470. The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. NBP2-61470 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.")
![ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - ELISA of single stranded DNA using NBP2-61470. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61470 was used as the primary antibody, and an alkaline phosphatase conjugated anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-61470_rabbit-monoclonal-5-methylcytosine-antibody-rm231-elisa-189202523381723.png)
![Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Flow Cytometry analysis of 5-MethylCytosine expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61470 (Blue) or with a negative control antibody](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-RM231-Flow-Cytometry-NBP2-61470-img0008.jpg)
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - MeDIP was performed using NBP2-61470 at a 2:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.](http://images.novusbio.com/fullsize/nbp2-61470_rabbit-monoclonal-5-methylcytosine-antibody-rm231-methylated-dna-immunoprecipitation-18920252337121.png)
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Immunocytochemical staining of HeLa cells using NBP2-61470 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-61470_rabbit-monoclonal-5-methylcytosine-antibody-rm231-immunocytochemistry-immunofluorescence-199202502431.png)
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - Human osteosarcoma (U2OS) cells were stained with the monoclonal antibody against 5-mC. Cells were fixed with 2.5% PFA in PBS for 30 minutes, permeabilized with 0.5% Triton X-100 for 1 hour and treated with 2N HCl for 1 hour followed by 2 x 5 minutes with 0.1 M borate buffer to depurinate the DNA. After blocking with PBS containing 0.1% Triton X-100 and 1% BSA, the cells were immunofluorescently labelled with the 5-mC antibody diluted 1:500 in blocking solution, followed by a goat anti-mouse antibody conjugated to Alexa488. Figure 3A: cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 uM mCTP (left) or with DAPI (right). Figure 3B: staining of the cells with the 5-mC antibody after incubation of the antibody with 50 uM hmCTP and with DAPI.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0005.jpg "Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - Human osteosarcoma (U2OS) cells were stained with the monoclonal antibody against 5-mC. Cells were fixed with 2.5% PFA in PBS for 30 minutes, permeabilized with 0.5% Triton X-100 for 1 hour and treated with 2N HCl for 1 hour followed by 2 x 5 minutes with 0.1 M borate buffer to depurinate the DNA. After blocking with PBS containing 0.1% Triton X-100 and 1% BSA, the cells were immunofluorescently labelled with the 5-mC antibody diluted 1:500 in blocking solution, followed by a goat anti-mouse antibody conjugated to Alexa488. Figure 3A: cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 uM mCTP (left) or with DAPI (right). Figure 3B: staining of the cells with the 5-mC antibody after incubation of the antibody with 50 uM hmCTP and with DAPI.")
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/Triton X-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:1000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0004.jpg)
![In-situ Hybridization: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - To detect methylated chromosomal regions, FISH was performed on metaphase chromosomes from HeLa cells using the antibody against 5-mC. The cells were blocked in metaphase by treatment with colcemid (0.1 ug/mL) for 1 - 2 hours, fixed overnight at -20C with ethanol/glacial acetic acid and treated with 2N HCl for 30 minutes at room temperature. Subsequently, the cells were blocked with PBS containing 1% BSA and 0.1% Triton X-100 and stained with the 5-mC antibody (left) diluted 1:1,000 in blocking solution, followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the chromosomes with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0002.jpg)
![Dot Blot: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - To demonstrate the specificity of the antibody against 5-mC, a Dot blot analysis was performed using hmC, mC and C controls. 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:600. Dot blot shows a high specificity of the antibody for the methylated control.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Dot-Blot-NBP2-59166-img0003.jpg)
![Immunofluorescence: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200006-Immunofluorescence-NBP2-59170-img0002.jpg "Immunofluorescence: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170] - Methylated DNA immunoprecipitation was performed on 1 ug fragmented human genomic DNA using 0.2 ug of the antibody against 5-mC and a MeDIP Kit. The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200006-Methylated-DNA-Immunoprecipitation-NBP2-59170-img0001.jpg)
![Dot Blot: 5-MethylCytosine Antibody [NBP2-42813] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:4000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-Dot-Blot-NBP2-42813-img0001.jpg "Dot Blot: 5-MethylCytosine Antibody [NBP2-42813] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:4000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.")
![Dot Blot: 5-MethylCytosine Antibody (10S5V10) [NBP3-35058] - Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody at 1:1000 dilution.5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3' unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'](http://images.novusbio.com/fullsize/nbp3-35058_rabbit-5-methylcytosine-mab-10s5v10-10122024140430.jpg "Dot Blot: 5-MethylCytosine Antibody (10S5V10) [NBP3-35058] - Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody at 1:1000 dilution.5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3' unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'")