5-MethylCytosine Antibody (RM231)

Images

 
Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Immunocytochemical staining of HeLa cells using Anti-5-MethylCytosine antibody (NBP2-61470) (red). Actin filaments have been ...read more
Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Flow Cytometry analysis of 5-MethylCytosine expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% ...read more
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - ELISA of single stranded DNA using Anti-5-MethylCytosine antibody (NBP2-61470). The plate was coated with streptavidin and then biotinylated single stranded ...read more
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Direct ELISA of HeLa cell genomic DNA using Anti-5-MethylCytosine antibody (NBP2-61470). The plate was directly coated with different concentrations of genomic DNA ...read more
Dot Blot: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Dot blot of double stranded DNA using rabbit monoclonal NBP2-61470 antibody. The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine 5-hmC) ...read more
Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - MeDIP was performed using Anti-5-MethylCytosine antibody (NBP2-61470) at a 2:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine ...read more

Product Details

Summary
Reactivity VbSpecies Glossary
Applications DB, ELISA, Flow, ICC/IF, IHC, MeDIP
Clone
RM231
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

5-MethylCytosine Antibody (RM231) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
BSA-conjugated 5-MethylCytosine
Specificity
This antibody reacts to 5-MethylCytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot 0.5 ug/ml - 2 ug/ml
  • ELISA 0.1 ug/ml - 1 ug/ml
  • Flow Cytometry 0.5 ug/ml-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.2 ug/ml - 2 ug/ml
  • Immunohistochemistry
  • Methylated DNA Immunoprecipitation 0.2 ug/ml - 2 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/PBS with 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for 5-MethylCytosine Antibody (RM231)

  • 5-mC
  • 5-Methyl Cytosine
  • Cytosine (5-Methyl)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-MethylCytosine Antibody (NBP2-61470) (0)

There are no publications for 5-MethylCytosine Antibody (NBP2-61470).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 5-MethylCytosine Antibody (NBP2-61470) (0)

There are no reviews for 5-MethylCytosine Antibody (NBP2-61470). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for 5-MethylCytosine Antibody (NBP2-61470) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 5-MethylCytosine Antibody (RM231) and receive a gift card or discount.