Epiregulin Antibody [Unconjugated]

Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proepiregulin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068). The ND50 is typically 0.3-1 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Epiregulin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Epiregulin
  Val56-Leu101
  Accession # Q61521
• Specificity: Detects mouse Epiregulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Epiregulin is observed. In Western blots, recombinant mouse Proepiregulin and mature Epiregulin is detected.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EREG
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.3-1.0 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Epiregulin Antibody [Unconjugated]

• Epiregulin
• ER
• EREG
• proepiregulin

Background

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a transmembrane precursor of 162 amino acid in length, with the mature soluble form comprising residues 56-101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).

1. Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
2. Toyoda, H. et al. (1995) FEBS Lett. 377:403.
3. Komurasaki, T. et al. (1997) Oncogene 15:2841.
4. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
5. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
6. Das, S.K. et al. (1997) Dev. Biol. 190:178.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Epiregulin Antibody (AF1068)(4)

Publications using AF1068

• S.E. Tahaei, G. Couasnay, Y. Ma, N. Paria, J. Gu, B. F. Lemoine et al. The reduced osteogenic potential of Nf1-deficient osteoprogenitors is EGFR-independent Bone 1/1/2018 [PMID: 29032173]
• S.E. Tahaei, G. Couasnay, Y. Ma, N. Paria, J. Gu, B. F. Lemoine et al. The reduced osteogenic potential of Nf1-deficient osteoprogenitors is EGFR-independent Bone 2018-01-01 [PMID: 29032173] (Western Blot, Neutralization, Human, Mouse)
• Lindsey K. Bade, Jodi E. Goldberg, Hazel A. DeHut, Majken K. Hall, Kathryn L. Schwertfeger Mammary tumorigenesis induced by fibroblast growth factor receptor 1 requires activation of the epidermal growth factor receptor Journal of Cell Science 9/15/2011 [PMID: 21868365]
• Andric N, Ascoli M The luteinizing hormone receptor-activated extracellularly regulated kinase-1/2 cascade stimulates epiregulin release from granulosa cells. Endocrinology, 2008-07-24;149(11):5549-56. 2008-07-24 [PMID: 18653716] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: EREG
• Uniprot:
  • Q61521()