CCL7/MCP-3/MARC Products

456-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/ MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND₅₀ is typically 7.5-45 µg/mL." class="big_lightbox" target="_blank">

282-P3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL7/MCP-3 Monoclonal Antibody (Catalog # MAB282). The ND₅₀ is typically 1.5-4.5 µg/mL." class="big_lightbox" target="_blank">

282-P3) was serially diluted and captured by Mouse Anti-Human CCL7/MCP‑3/MARC Monoclonal Antibody (Catalog # MAB282) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL7/MCP‑3/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-282-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">