Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2328-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2897-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2200U-100UG | |||
| Alexa Fluor 405 | FAB2200V-100UG | |||
| Alexa Fluor 488 | FAB2200G-100UG | |||
| Alexa Fluor 532 | FAB2200X-100UG | |||
| Alexa Fluor 594 | FAB2200T-100UG | |||
| Alexa Fluor 647 | FAB2200R-100UG | |||
| Alexa Fluor 700 | FAB2200N-100UG | |||
| Alexa Fluor 750 | FAB2200S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2200-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3845-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26556-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB3054U-100UG | |||
| Alexa Fluor 405 | FAB3054V-100UG | |||
| Alexa Fluor 488 | FAB3054G-100UG | |||
| Alexa Fluor 532 | FAB3054X-100UG | |||
| Alexa Fluor 594 | FAB3054T-100UG | |||
| Alexa Fluor 647 | FAB3054R-100UG | |||
| Alexa Fluor 700 | FAB3054N-100UG | |||
| Alexa Fluor 750 | FAB3054S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3054-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB4262U-100UG | |||
| Alexa Fluor 405 | FAB4262V-100UG | |||
| Alexa Fluor 488 | FAB4262G-100UG | |||
| Alexa Fluor 532 | FAB4262X-100UG | |||
| Alexa Fluor 594 | FAB4262T-100UG | |||
| Alexa Fluor 647 | FAB4262R-100UG | |||
| Alexa Fluor 700 | FAB4262N-100UG | |||
| Alexa Fluor 750 | FAB4262S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4262-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF73091-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6087-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12091-0.025mg | |||
![Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] - Immunoprecipitating GDNF in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26556 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26556 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26556_rabbit-gdnf-mab-11d2-262202413221516.jpg "Immunoprecipitation: GDNF Antibody (11D2) [NBP3-26556] - Immunoprecipitating GDNF in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26556 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26556 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26556_rabbit-gdnf-mab-11d2-262202414471592.jpg)
![Immunohistochemistry: GDNF Antibody (11D2) [NBP3-26556] - Image of GDNF Antibody (11D2) diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26556_rabbit-gdnf-mab-11d2-262202415355747.jpg)
![Western Blot: GDNF Antibody (11D2) [NBP3-26556] - Positive Western Blot detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate. All lanes: GDNF Antibody at 1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 21, 26, 23, 19 KDa Observed band size: 24 KDa](http://images.novusbio.com/fullsize/nbp3-26556_rabbit-gdnf-mab-11d2-28220241140933.jpg)
![Flow Cytometry: GDNF Antibody (11D2) [NBP3-26556] - Overlay histogram showing SH-SY5Y cells stained with GDNF Antibody (11D2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26556_rabbit-gdnf-mab-11d2-282202414183619.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:200](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0004.jpg "Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:200")
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0001.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining of human liver with NBP3-12091. Cytoplasm of hepatocytes moderately labelled and plasma of veins strongly in liver tissue. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0002.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining in human tonsil with NBP3-12091. Plasma in lumen of veins is stained strongly as well as follicular dendritic cells in germinal center of tonsil. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0003.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining in human tonsil with NBP3-12091. Plasma in lumen of veins is stained strongly in tonsil tissue. Dilution: 1:200](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0005.jpg)