Flow Cytometry is a laser based biological assay used for cell detection, counting and sorting of specific biomarkers. Cells are first suspended in solution and fluorescently labeled antibodies are applied. Then the cells are passed single-file through a series of lasers which analyze size, shape and the fluorescent characteristics of each cell. Results can then be electronically gated to filter the data to a specific cell type and whether or not they contain bound antibodies. This form of assay is particularly useful for analyzing blood samples.
Novus offers a range of support options for flow cytometry when you buy a product to help you with your flow cytometry experiment including over 1,000 Flow images using our pre-conjugated antibodies:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-16773 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0470A488-100 | |||
| Alexa Fluor 546 | DDX0470A546-100 | |||
| Alexa Fluor 647 | DDX0470A647-100 | |||
| Biotin | DDX0470B | |||
![Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Western-Blot-NB500-352-img0002.jpg "Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Immunohistochemistry-Paraffin-NB500-352-img0003.jpg)
![Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Intracellular-NB500-352-img0006.jpg)
![Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Fig. 1. Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7/17 Antibody (C-46).](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Immunohistochemistry-NB500-352-img0001.jpg)
![Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Cytometry-NB500-352-img0004.jpg)
![Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on A549 cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-7-17-Antibody-C-46-Flow-Cytometry-NB500-352-img0005.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0009.jpg "Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.")
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Raji cells with MS4A1/CD20 antibody (MEM-97) NBP1-44634AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0001.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Daudi cells with MS4A1/CD20 (MEM-97) antibody NBP1-44634PE (blue) along with a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0002.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibod](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0006.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Flow Cytometry: MS4A1/CD20 Antibody (MEM-97) [Alexa Fluor 647] [NBP1-44634AF647] - A surface stain was performed on human peripheral blood lymphocytes with MS4A1/CD20 (MEM-97) antibody NBP1-44634AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0007.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Ramos cells with CD20 antibody (MEM-97) NBP1-44634PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0008.jpg)
![Immunoprecipitation: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97)](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Immunoprecipitation-NBP1-44634-img0005.jpg)
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)
![Flow Cytometry: Lightning-Link PerCP Antibody Labeling Kit [718-0005]](http://images.novusbio.com/fullsize/Lightning-Link-PerCP-Antibody-Labeling-Kit-Flow-Cytometry-718-0005-img0001.jpg "Flow Cytometry: Lightning-Link PerCP Antibody Labeling Kit [718-0005]")
![Flow Cytometry: Lightning-Link PE-Cy7 Antibody Labeling Kit [762-0005]](http://images.novusbio.com/fullsize/Lightning-Link-PE-Cy7-Antibody-Labeling-Kit-Flow-Cytometry-762-0005-img0001.jpg "Flow Cytometry: Lightning-Link PE-Cy7 Antibody Labeling Kit [762-0005]")
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NB7588-img0006.jpg "Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7588-img0002.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0005.jpg)
![Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Flow-Cytometry-NB7588-img0004.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0008.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0007.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NBP2-21581-img0017.jpg "Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.")
![Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Flow-Cytometry-NBP2-21581-img0013.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunohistochemistry-Paraffin-NBP2-14890-img0009.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry analysis.Black: Unlabelled sample was used as a control.Red: BrdU antibodyAcquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Flow-Cytometry-NBP2-14890-img0004.jpg)
![Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Western-Blot-DDX0470P-100-img0007.jpg "Western Blot: TLR3 Antibody (619F7.06) [DDX0470P-100] - Western-blot analysisof human (h) and canine(c) PBMC and monocytes with 619F7.06")
![Immunohistochemistry-Frozen: TLR3 Antibody (619F7.06) [DDX0470P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0470P-100. Staining of human tonsil section.](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Frozen-DDX0470P-100-img0003.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of canine blood cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR3 Antibody (619F7.06) [DDX0470P-100] - Paraffin staining of Canine keratinocytes with TLR3 Antibody](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Immunohistochemistry-Paraffin-DDX0470P-100-img0001.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human breast carcinoma CAMA-1 cellline with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0004.jpg)
![Flow Cytometry: TLR3 Antibody (619F7.06) [DDX0470P-100] - Intracellular FACS staining of human monocyte-derived DCs and human EBV B cells with 619F7.06](http://images.novusbio.com/fullsize/TLR3-Antibody-619F7-06-Flow-Cytometry-DDX0470P-100-img0005.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-15053-img0015.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: OCT4 Antibody [NBP2-15053] - Human embryonic stem cells, staining with OCT3/4 or rabbit IgG (black).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NBP2-15053-img0001.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0007.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP2-15053] - Analysis of hESCs on CF1 feeders, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15053-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody [NBP2-15053] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15053_rabbit-polyclonal-oct4-antibody-1610202419414555.jpg)
