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Product Search for ELISA

Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.

Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:

54764 Results for "ELISA"

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KGE012B

Mouse/Rat cAMP Parameter Assay Kit

(35 Publications)

1 Kit

KGE012B

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

Applications:

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MCTC0

Rat/Mouse Cytochrome c Quantikine ELISA Kit

(35 Publications)

1 Kit

MCTC0

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

Applications:

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NB110-40686

CD163 Antibody (EDHu-1) - BSA Free

(4 Reviews)

(28 Publications)

0.1 mg

NB110-40686

CD163 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

CD163 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 20 µg/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

CD163 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

Non-repaired and repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker, green) and wheat germ agglutinin (WGA, red) and DAPI (nuclei, blue). Examples for each group, specifically the non-, SIS-, UMB-, and Hya-reparied are shown. Scale bar is 150 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-13306-2), licensed under a CC-BY license.

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686AF700 and a matched isotype control NBP2-27287AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.

Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

FFPE human tonsil, stained with anti-CD163, pH 9 antigen retrieval. IHC-P Image submitted by a verified customer review.

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686PE and a matched isotype control NBP2-27287PE. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758APC. Image using the PE form of this antibody.

Human tonsil cryosection with Mouse anti Human CD163 antibody, clone, red in A and Mouse anti Human CD21 antibody, green in B. C is the merged image with nuclei counterstained blue using DAPI.

Frozen human skin sections. CD163 (green) in control human skin. Sections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was diluted 1:100 and applied for 1 hr at 37C. Detection was performed with anti-host conjugated antibody, and sections were counterstained with DAPI. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in a formalin fixed paraffin embedded section of canine lymph node. NB110-40686 was diluted to 0.25ug per mL and was left on the tissue section for 30m at room temperature. Section underwent 20m of heat induced epitope retrieval in a vegetable steamer using a citrate buffer. No signal was detected in sections that did not undergo HIER. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of human tonsil. NB110-40686 was diluted to 250 ng/mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of equine spleen. NB110-40686 was diluted to 250ng per mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired & repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker) & wheat germ agglutinin (WGA) & DAPI (nuclei). (a) For each of the sham muscle sections individual & merged images are presented. (b) Examples for each group, specifically the non-, SIS-, UMB-, & Hya-reparied are shown. Scale bar is 150 µm; all images are at the same magnification & follow the same location pattern displayed specifically in Fig. 4e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-40686AF350
Alexa Fluor 405	NB110-40686AF405
Alexa Fluor 488	NB110-40686AF488
Alexa Fluor 532	NB110-40686AF532
Alexa Fluor 594	NB110-40686AF594
Alexa Fluor 647	NB110-40686AF647
Alexa Fluor 700	NB110-40686AF700
Alexa Fluor 750	NB110-40686AF750
Allophycocyanin	NB110-40686APC
Allophycocyanin/Cy7	NB110-40686APCCY7
Biotin	NB110-40686B
CoraFluor 1	NB110-40686CL1
DyLight 350	NB110-40686UV
DyLight 405	NB110-40686V
DyLight 488	NB110-40686G
DyLight 550	NB110-40686R
DyLight 594	NB110-40686DL594
DyLight 650	NB110-40686C
DyLight 680	NB110-40686FR
DyLight 755	NB110-40686IR
FITC	NB110-40686F
HRP	NB110-40686H
Janelia Fluor 525	NB110-40686JF525
Janelia Fluor 549	NB110-40686JF549
Janelia Fluor 646	NB110-40686JF646
Janelia Fluor 669	NB110-40686JF669
PE	NB110-40686PE
PE/Atto594	NB110-40686PEATT594
PE/Cy5.5	NB110-40686PECY55
PE/Cy7	NB110-40686PECY7
PerCP	NB110-40686PCP
mFluor Violet 450 SE	NB110-40686MFV450
mFluor Violet 500 SE	NB110-40686MFV500
mFluor Violet 610 SE	NB110-40686MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-40686-0.025mg

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KGE014

Estradiol Parameter Assay Kit

(35 Publications)

1 Kit

KGE014

Reactivity:

All-Multi

Species Glossary

Reactivity: All-Multi

Applications:

Applications:

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P8000

Porcine IL-8/CXCL8 Quantikine ELISA Kit

(35 Publications)

1 Kit

P8000

Reactivity:

Species Glossary

Reactivity: Po

Applications:

Applications:

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DY528

Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA, 15 Plate

(36 Publications)

1 Kit (for 15 Plates)

DY528

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

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DTX00

Human CCL11/Eotaxin Quantikine ELISA Kit

(34 Publications)

1 Kit

DTX00

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	STX00
HRP	PDTX00

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H00055669-M04

Mitofusin 1 Antibody (3C9) - Azide and BSA Free

(33 Publications)

0.1 mg

H00055669-M04

Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with H00055669-M04. GAPDH (36.1 kDa) used as loading control.

Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: Non-transfected lysate.

ELISA: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Detection limit for recombinant GST tagged MFN1 is 0.3 ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00029978-M03

Ubiquilin 2 Antibody (5F5) - Azide and BSA Free

(1 Review)

(29 Publications)

0.1 mg

H00029978-M03

Immunocytochemistry/Immunofluorescence: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of monoclonal antibody to UBQLN2 on A-431 cell. Antibody concentration 10 ug/ml

Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in PC-12 (Cat # L012V1).

Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in Raw 264.7 (Cat # L024V1).

Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in A-431 (Cat # L015V1).

<b>Genetic Strategies Validation. </b>Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - UBQLN2 shRNA knockdown in SH-SY5Y cells. First lane is the ladder, then treatment with scrambled shRNA and third lane is UBQLN2 knockdown. 30 ug of the protein sample was loaded on the gel and transferred to the nitrocellulose membrane. Overnight incubation in primary antibodies at 4 degrees C in 5 % milk in TBST. Then incubated in secondary antibodies (Goat Anti-mouse DyLight 550) at RT for 2 h, and then imaged. Knockdown of the upper band with shRNA for UBQLN2 was about 82 %, and of the lower band only about 8 %, suggesting a high specificity of the used shRNA. This image was submitted via customer Review.

ELISA: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Detection limit for recombinant GST tagged UBQLN2 is approximately 0.03ng/ml as a capture antibody.

Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Generation of transgenic mice that overexpress human ubiquilin-1.(A) Schematic of the Thy1.2 expression construct used to generate ubiquilin-1 transgenic mice. Human ubiquilin-1 with an N-terminal FLAG tag was cloned in the appropriate orientation between the XhoI site of the Thy1.2 expression cassette. (B) Southern Blot of the first generation offspring of two founder mice (48 & 62). (C) Validation of a PCR genotyping protocol. Amplification of the transgene was only observed in mice that Southern blotting revealed to be positive. (D) Immunoblots of brain cortical lysates with an anti-FLAG antibody & for tubulin indicated that line 62 offspring express higher levels of FLAG-ubiquilin-1 than line 48. (E) Immunoblots of equal amounts of total brain lysates from 12 month-old WT mouse, 12 month-old Ubqln-1 48 transgenic mouse, 12 month-old Ubqln-1 62 transgenic mouse & end stage 15 week-old R6/2 transgenic mouse. The top panel was probed with a monoclonal anti-ubiquilin antibody (Invitrogen antibody clone 3D5E2) & the lower panel with a different monoclonal anti-ubiquilin antibody (Novus antibody clone 5F5). Note two immunoreactive ubiquilin bands are seen at ∼70 kDa & at ∼90 kDa, which we presume is a modified form of ubiquilin. Both blots were also probed for actin to ensure equal loading. (F) Cryostat sections of a Ubqln-1 62 transgenic mouse brain (a–f) & WT mouse brain (g-i) showing anti-FLAG antibody staining (Alexa 594, left panels) & corresponding DAPI staining (center panels) & the result of merging the fluorescent & DAPI signals (right hand panels). The brain sections shown are of the hippocampus (a–c & g–i) & cerebellum (d–f). Identical exposure settings were used for the left hand panels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24475300), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Confirmation of polyQ-associated proteins from PC-12 cells identified by TAPI.(A) Western blotting shows that the addition of doxycycline to the PC-12 cell model induces the expression of HttQ74-GFP, resulting in aggregates that can be purified by TAPI. The kinase ERK is probed as a negative control; ERK was never identified by mass spectrometry, so is not expected to co-fractionate with polyQ aggregates. (B) Western blot analysis of TAPI-purified polyQ aggregates from PC-12 cells confirms the presence of several disease-associated proteins only in the Htt-Q74 samples. All proteins migrated near their predicted molecular weights. For control, the TAPI procedure was conducted in parallel on the induced Htt-Q23 cell line (FUS, TDP-43, UBQLN2, HNRNPA1) or the un-induced Htt-Q74 cell line (CLINT1, HSPA8, RAD23B, SGTA). (C) Confocal microscopy shows localization of identified proteins to Htt-Q74 aggregates in PC12 cells. (left) RAD23B, nominally a DNA repair protein, localizes to nuclear Htt-Q74 inclusions but not cytoplasmic inclusions. (middle) FUS, an RNA-binding protein localizes to nuclear & cytoplasmic Htt-Q74 inclusions. (right) CLINT1, a clatherin-interacting protein, is observed in cytoplasmic Htt-Q74 aggregates. Arrows indicate foci with co-localized proteins. Green = GFP; Magenta = CLINT1, FUS or RAD23B in merge. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0136362), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MMC00B

Mouse M-CSF Quantikine ELISA Kit

(34 Publications)

1 Kit

MMC00B

M-CSF is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 103% compared to 84% for the top competitor. Cell culture supernates recovery is 100% compared to 75% for the top competitor.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NB7276

Goat anti-Chicken IgM Heavy Chain Secondary Antibody

(37 Publications)

1 ml

Reactivity:

Species Glossary

Reactivity: Ch

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	NB7276APC
Biotin	NB7276B
DyLight 350	NB7276UV
DyLight 405	NB7276V
DyLight 488	NB7276G
DyLight 550	NB7276R
DyLight 594	NB7276DL594
DyLight 650	NB7276C
DyLight 680	NB7276FR
DyLight 755	NB7276IR
FITC	NB7278
HRP	NB7279
Janelia Fluor 525	NB7276JF525
Janelia Fluor 669	NB7276JF669
PE	NB7276PE
PerCP	NB7276PCP

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D1300B

Human IL-13 Quantikine ELISA Kit

(32 Publications)

1 Kit

D1300B

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PD1300B
HRP	S1300B

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	PD1300B
Carrier Free	S1300B

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Product Search for ELISA

Mouse/Rat cAMP Parameter Assay Kit

Rat/Mouse Cytochrome c Quantikine ELISA Kit

CD163 Antibody (EDHu-1) - BSA Free

Estradiol Parameter Assay Kit

Porcine IL-8/CXCL8 Quantikine ELISA Kit

Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA, 15 Plate

Human CCL11/Eotaxin Quantikine ELISA Kit

Mitofusin 1 Antibody (3C9) - Azide and BSA Free

Ubiquilin 2 Antibody (5F5) - Azide and BSA Free

Mouse M-CSF Quantikine ELISA Kit

Goat anti-Chicken IgM Heavy Chain Secondary Antibody

Human IL-13 Quantikine ELISA Kit