Mouse/Rat cAMP Parameter Assay Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Mouse/Rat cAMP Parameter Assay Kit Summary

• Background: The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.
• Specificity: Cyclic AMP in mouse and rat samples
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat cAMP Parameter Assay Kit

• cAMP
• Cyclic AMP

Background

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for cAMP (KGE012B)(35)

Publications using KGE012B

• Chen, M;Lu, L;Cheng, D;Zhang, J;Liu, X;Zhang, J;Zhang, T; Icariin Promotes Osteogenic Differentiation in a Cell Model with NF1 Gene Knockout by Activating the cAMP/PKA/CREB Pathway Molecules 2023-01-01 [PMID: 37446790] (Mouse)
• Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G; Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling Respiratory research 2023-07-13 [PMID: 37438813] (Mouse)
• Wang, JH;Hwang, SJ;Lee, SK;Choi, Y;Byun, CK;Son, CG; Anti-Melanogenic Effects of Fractioned Cynanchum atratum by Regulation of cAMP/MITF Pathway in a UVB-Stimulated Mice Model Cells 2023-05-14 [PMID: 37408224] (Mouse)
• T Gwag, D Li, E Ma, Z Guo, Y Liang, S Wang CD47 antisense oligonucleotide treatment attenuates obesity and its-associated metabolic dysfunction Scientific Reports, 2023-02-16;13(1):2748. 2023-02-16 [PMID: 36797364] (Mouse)
• Q Chen, L Huang, D Pan, K Hu, R Li, RH Friedline, JK Kim, LJ Zhu, DA Guertin, YX Wang A brown fat-enriched adipokine Adissp controls adipose thermogenesis and glucose homeostasis Nature Communications, 2022-12-10;13(1):7633. 2022-12-10 [PMID: 36496438] (Mouse)
• Y Hu, LJ Fan, YM Jiang, H Liu, H Yong, C Peng Intrathecal Injection of Ropivacaine Reduces Cervical Resistance in Late-Pregnant Rats Drug Design, Development and Therapy, 2022-04-26;16(0):1183-1189. 2022-04-26 [PMID: 35502424] (Rat)
• S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser Deiodinase-3 is a thyrostat to regulate podocyte homeostasis EBioMedicine, 2021-10-11;72(0):103617. 2021-10-11 [PMID: 34649077] (Mouse)
• YJ Kim, JS Lee, H Kim, JH Jang, YH Choung Gap Junction-Mediated Intercellular Communication of cAMP Prevents CDDP-Induced Ototoxicity via cAMP/PKA/CREB Pathway International Journal of Molecular Sciences, 2021-06-13;22(12):. 2021-06-13 [PMID: 34199197] (Mouse)
• J Amarù, F Barbieri, M Arvigo, A Solari, A Bajetto, F Nista, C Campana, G Gaggero, A Prior, D Criminelli, G Zona, D Ferone, T Florio, F Gatto Octreotide and Pasireotide Combination Treatment in Somatotroph Tumor Cells: Predominant Role of SST2 in Mediating Ligand Effects Cancers, 2021-04-10;13(8):. 2021-04-10 [PMID: 33920241] (Rat)
• N Azzam, R Bar-Shalom, F Fares A Novel Follitropin Analog Inhibits Follitropin Activity In Vitro Pharmaceutics, 2021-03-03;13(3):. 2021-03-03 [PMID: 33802415] (Hamster)
• M Huang, Y Lin, L Wang, X You, S Wang, J Zhao, M Bai, Z Li, Y Chen Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D Molecular Metabolism, 2021-02-05;47(0):101182. 2021-02-05 [PMID: 33549845] (Mouse)
• YQ Ping, C Mao, P Xiao, RJ Zhao, Y Jiang, Z Yang, WT An, DD Shen, F Yang, H Zhang, C Qu, Q Shen, C Tian, ZJ Li, S Li, GY Wang, X Tao, X Wen, YN Zhong, J Yang, F Yi, X Yu, HE Xu, Y Zhang, JP Sun Structures of the glucocorticoid-bound adhesion receptor GPR97-Go complex Nature, 2021-01-06;0(0):. 2021-01-06 [PMID: 33408414] (Mouse)
• S Makris, C Johansson R848 or influenza virus can induce potent innate immune responses in the lungs of neonatal mice Mucosal Immunol, 2020-06-23;0(0):. 2020-06-23 [PMID: 32576926] (Mouse)
• S Xiong, Z Hong, LS Huang, Y Tsukasaki, S Nepal, A Di, M Zhong, W Wu, Z Ye, X Gao, GN Rao, D Mehta, J Rehman, AB Malik IL-1beta suppression of VE-cadherin transcription underlies sepsis-induced inflammatory lung injury J. Clin. Invest., 2020-07-01;130(7):3684-3698. 2020-07-01 [PMID: 32298238] (Mouse)
• M Xu, Z Jiang, C Wang, N Li, L Bo, Y Zha, J Bian, Y Zhang, X Deng Acetate attenuates inflammasome activation through GPR43-mediated Ca2+-dependent NLRP3 ubiquitination Exp. Mol. Med., 2019-07-23;51(7):83. 2019-07-23 [PMID: 31337751] (Mouse)
• SA El Awdan, RF Abdel Rahm, HM Ibrahim, RR Hegazy, SA El Marasy, M Badawi, MS Arbid Regression of fibrosis by cilostazol in a rat model of thioacetamide-induced liver fibrosis: Up regulation of hepatic cAMP, and modulation of inflammatory, oxidative stress and apoptotic biomarkers PLoS ONE, 2019-05-08;14(5):e0216301. 2019-05-08 [PMID: 31067255] (Rat)
• L Shen, TR Xie, RZ Yang, Y Chen, JS Kang Application of a dye-based mitochondrion-thermometry to determine the receptor downstream of prostaglandin E2 involved in the regulation of hepatocyte metabolism Sci Rep, 2018-08-30;8(1):13065. 2018-08-30 [PMID: 30166566] (Mouse)
• S Eiam-Ong, Y Nakchui, M Chaipipat, S Eiam-Ong Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels Toxicol Res, 2018-04-15;34(2):143-150. 2018-04-15 [PMID: 29686776] (Rat)
• PA Harnedy, V Parthsarat, CM McLaughlin, MB O'Keeffe, PJ Allsopp, EM McSorley, FPM O'Harte, RJ FitzGerald Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides Food Res. Int., 2018-01-16;106(0):598-606. 2018-01-16 [PMID: 29579965] (Rat)
• X Yang, G Wen, B Tuo, F Zhang, H Wan, J He, S Yang, H Dong Molecular mechanisms of calcium signaling in the modulation of small intestinal ion transports and bicarbonate secretion Oncotarget, 2017-12-11;9(3):3727-3740. 2017-12-11 [PMID: 29423078] (Mouse)
• DL Zhang, YJ Sun, ML Ma, YJ Wang, H Lin, RR Li, ZL Liang, Y Gao, Z Yang, DF He, A Lin, H Mo, YJ Lu, MJ Li, W Kong, KY Chung, F Yi, JY Li, YY Qin, J Li, ARB Thomsen, AW Kahsai, ZJ Chen, ZG Xu, M Liu, D Li, X Yu, JP Sun Gq activity- and ?-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility Elife, 2018-02-02;7(0):. 2018-02-02 [PMID: 29393851] (Mouse)
• B Ogola, Y Zhang, L Iyer, T Thekkumkar 2-Methoxyestradiol Causes MMP 9 Mediated Transactivation of EGFR and Angiotensin Type 1 Receptor Downregulation in Rat Aortic Smooth Muscle Cells Am. J. Physiol., Cell Physiol., 2018-01-24;0(0):. 2018-01-24 [PMID: 29365274] (Rat)
• Y Qiu, Y Wang, X Wang, C Wang, ZY Xia Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats J. Int. Med. Res., 2018-01-14;0(0):3000605177440. 2018-01-14 [PMID: 29332488] (Rat)
• AK Dilly, BD Honick, YJ Lee, ZS Guo, HJ Zeh, DL Bartlett, HA Choudry Targeting G-protein coupled receptor-related signaling pathway in a murine xenograft model of appendiceal pseudomyxoma peritonei Oncotarget, 2017-11-06;8(63):106888-106900. 2017-11-06 [PMID: 29290997] (Mouse)
• R Shi, ZT Xiao, YJ Zheng, YL Zhang, JW Xu, JH Huang, WL Zhou, PB Li, WW Su Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells Cell. Physiol. Biochem., 2017-11-27;44(3):1146-1160. 2017-11-27 [PMID: 29179179] (Rat)
• YC Chen, CC Ho, CH Yi, XZ Liu, TT Cheng, CF Lam Exendin-4, a glucagon-like peptide-1 analogue accelerates healing of chronic gastric ulcer in diabetic rats PLoS ONE, 2017-11-02;12(11):e0187434. 2017-11-02 [PMID: 29095895] (Rat)
• D Yan, C Jin, Y Cao, L Wang, X Lu, J Yang, S Wu, Y Cai Effects of Aluminium on long-term Memory in Rats and on SIRT1 Mediating the Transcription of CREB-Dependent Gene in Hippocampus Basic Clin. Pharmacol. Toxicol., 2017-06-19;0(0):. 2017-06-19 [PMID: 28429887] (Rat)
• S Arab, N Kheshtchin, M Ajami, M Ashurpoor, A Safvati, A Namdar, R Mirzaei, N Mousavi Ni, F Jadidi-Nia, MH Ghahremani, J Hadjati Increased efficacy of a dendritic cell-based therapeutic cancer vaccine with adenosine receptor antagonist and CD73 inhibitor Tumour Biol, 2017-03-01;39(3):1010428317695. 2017-03-01 [PMID: 28349824] (Mouse)
• TF Li, HY Wu, YR Wang, XY Li, YX Wang Molecular signaling underlying bulleyaconitine A (BAA)-induced microglial expression of prodynorphin Sci Rep, 2017-03-22;7(0):45056. 2017-03-22 [PMID: 28327597] (Rat)
• HY Wu, XF Mao, H Fan, YX Wang p38? MAPK signaling mediates exenatide-stimulated microglial ?-endorphin expression Mol. Pharmacol, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202578] (Rat)
• Zhonghao Liu Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats Can J Diabetes, 2016-11-23;0(0):. 2016-11-23 [PMID: 27889175] (Rat)
• Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice Sci Rep, 2016-07-29;6(0):30887. 2016-07-29 [PMID: 27471110] (Mouse)
• Pan , Dongning, Mao , Chunxiao, Quattrochi , Brian, Friedline , Randall, Zhu , Lihua J, Jung , Dae Youn, Kim , Jason K, Lewis , Brian, Wang , Yong-Xu MicroRNA-378 controls classical brown fat expansion to counteract obesity. Nat Commun, 2014-08-22;5(0):4725. 2014-08-22 [PMID: 25145289] (Mouse)
• Hinman M, Sharma A, Luo G, Lou H Neurofibromatosis type 1 alternative splicing is a key regulator of Ras signaling in neurons. Mol Cell Biol, 2014-04-07;34(12):2188-97. 2014-04-07 [PMID: 24710274] (Mouse)
• Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury. J Immunol, 2014-03-19;192(8):3765-77. 2014-03-19 [PMID: 24646745] (Rat)