Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77319-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76981-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41208-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77248-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65510-0.05ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76645-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76507-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41249-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76939-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77297-0.025mg | |||
![Western Blot: SLITRK1 Antibody [NBP1-77319] - Western blot analysis of SLITRK1 in human brain tissue lysate with SLITRK1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SLITRK1-Antibody-Western-Blot-NBP1-77319-img0008.jpg "Western Blot: SLITRK1 Antibody [NBP1-77319] - Western blot analysis of SLITRK1 in human brain tissue lysate with SLITRK1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SLITRK1 Antibody [NBP1-77319] - Immunofluorescence of Slitrk1 in mouse brain tissue with Slitrk1 Antibody at 20 ug/mL. Green: Slitrk1 antibody (NBP1-77319). Red: Phylloidin staining. Blue: DAPI staining.](http://images.novusbio.com/fullsize/SLITRK1-Antibody-Immunohistochemistry-NBP1-77319-img0007.jpg)
![Immunohistochemistry: SLITRK1 Antibody - BSA Free [NBP1-77319] - Immunofluorescence of slitrk1 in human brain tissue with slitrk1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77319_rabbit-polyclonal-slitrk1-antibody-2711202420212341.jpg)
![Immunohistochemistry: SLITRK1 Antibody - BSA Free [NBP1-77319] - Immunohistochemistry of SLITRK1 in mouse brain tissue with SLITRK1 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77319_rabbit-polyclonal-slitrk1-antibody-2711202420333862.gif)
![Immunohistochemistry: SLITRK1 Antibody - BSA Free [NBP1-77319] - Immunohistochemistry of SLITRK1 in human brain tissue with SLITRK1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77319_rabbit-polyclonal-slitrk1-antibody-271120242016926.jpg)
![Western Blot: RNASEH2A Antibody [NBP1-76981] - HeLa cell lysate with RNAse H2A antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/RNASEH2A-Antibody-Western-Blot-NBP1-76981-img0001.jpg "Western Blot: RNASEH2A Antibody [NBP1-76981] - HeLa cell lysate with RNAse H2A antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981] - Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76981_rabbit-polyclonal-rnaseh2a-antibody-2711202419475883.jpg)
![Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981] - Immunofluorescence of RNase H2A in Hela cells with RNAse H2A antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76981_rabbit-polyclonal-rnaseh2a-antibody-2711202419592059.jpg)
![Western Blot: STAT3 Antibody [NBP2-41208] - Western blot analysis of STAT3 in human small intestine tissue lysate with STAT3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/STAT3-Antibody-Western-Blot-NBP2-41208-img0001.jpg "Western Blot: STAT3 Antibody [NBP2-41208] - Western blot analysis of STAT3 in human small intestine tissue lysate with STAT3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: STAT3 Antibody - BSA Free [NBP2-41208] - Immunofluorescence of STAT3 in human small intestine tissue with STAT3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41208_rabbit-polyclonal-stat3-antibody-271120241923614.jpg)
![Immunohistochemistry: STAT3 Antibody - BSA Free [NBP2-41208] - Immunohistochemistry of STAT3 in human small intestine tissue with STAT3 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41208_rabbit-polyclonal-stat3-antibody-2711202420115556.jpg)
![Western Blot: TRAF-2 Antibody [NBP1-77248] - Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Western-Blot-NBP1-77248-img0004.jpg "Western Blot: TRAF-2 Antibody [NBP1-77248] - Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NBP1-77248] - Immunofluorescence of TRAF2 in Hela cells with TRAF2 antibody at 20 ug/mL. Green: TRAF2 Antibody (NBP1-77248) Blue: DAPI staining](http://images.novusbio.com/fullsize/TRAF-2-Antibody-Immunofluorescence-NBP1-77248-img0009.jpg)
![Immunohistochemistry: TRAF-2 Antibody - BSA Free [NBP1-77248] - Immunohistochemistry of TRAF-2 in human liver tissue with TRAF-2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77248_rabbit-polyclonal-traf-2-antibody-2711202420272456.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAF-2 Antibody - BSA Free [NBP1-77248] - Immunofluorescence of TRAF-2 in human liver tissue with TRAF-2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77248_rabbit-polyclonal-traf-2-antibody-271120242016948.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (053A-1149.3.1.4) [NB100-65510] - Immunohistochemistry staining for C3d in human renal biopsy. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-053A-1149-3-1-4-Immunohistochemistry-Paraffin-NB100-65510-img0001.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (053A-1149.3.1.4) [NB100-65510] - Immunohistochemistry staining for C3d in human renal biopsy. IHC-P image submitted by a verified customer review.")
![Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.](http://images.novusbio.com/fullsize/GRG-Groucho-homolog-Antibody-Western-Blot-NBP1-76645-img0005.jpg "Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunofluorescence of GRG (Groucho homolog) in Hela cells with GRG (Groucho homolog) antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-2711202419475890.jpg)
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells with GRG (Groucho homolog) antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-271120242022923.jpg)
![Western Blot: SLC39A10 Antibody [NBP1-76507] - Human spleen tissue lysate with SLC39A10 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SLC39A10-Antibody-Western-Blot-NBP1-76507-img0001.jpg "Western Blot: SLC39A10 Antibody [NBP1-76507] - Human spleen tissue lysate with SLC39A10 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SLC39A10 Antibody - BSA Free [NBP1-76507] - Immunohistochemistry of SLC39A10 in human spleen tissue with SLC39A10 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76507_rabbit-polyclonal-slc39a10-antibody-2711202419475815.jpg)
![Immunocytochemistry/ Immunofluorescence: SLC39A10 Antibody - BSA Free [NBP1-76507] - Immunofluorescence of SLC39A10 in human spleen tissue with SLC39A10 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76507_rabbit-polyclonal-slc39a10-antibody-2711202419463497.jpg)
![Western Blot: Arrestin 3 Antibody [NBP2-41249] - Analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Western-Blot-NBP2-41249-img0001.jpg "Western Blot: Arrestin 3 Antibody [NBP2-41249] - Analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Arrestin 3 Antibody [NBP2-41249] - ARR3 in mouse spleen tissue with ARR3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Arrestin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41249-img0004.jpg)
![Immunohistochemistry: Arrestin 3 Antibody - BSA Free [NBP2-41249] - Immunohistochemistry of Arrestin 3 in mouse spleen tissue with Arrestin 3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41249_rabbit-polyclonal-arrestin-3-antibody-271120241955307.gif)
![Western Blot: OVGP1 Antibody [NBP1-76939] - Human placenta tissue lysate with OVGP1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/OVGP1-Antibody-Western-Blot-NBP1-76939-img0001.jpg "Western Blot: OVGP1 Antibody [NBP1-76939] - Human placenta tissue lysate with OVGP1 antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: OVGP1 Antibody - BSA Free [NBP1-76939] - Immunofluorescence of OVGP1 in Hek293 cells with OVGP1 antibody at 20 ug/mL.Green: OVGP1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76939_rabbit-polyclonal-ovgp1-antibody-2711202419553073.gif)
![Immunocytochemistry/ Immunofluorescence: OVGP1 Antibody - BSA Free [NBP1-76939] - Immunocytochemistry of OVGP1 in 293 cells with OVGP1L antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76939_rabbit-polyclonal-ovgp1-antibody-2711202419261839.jpg)
![Immunocytochemistry/ Immunofluorescence: OVGP1 Antibody - BSA Free [NBP1-76939] - Immunofluorescence of OVGP1 in 293 cells with OVGP1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76939_rabbit-polyclonal-ovgp1-antibody-271120241932622.jpg)
![Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419553086.gif "Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ASC/TMS1 (NBP1-77297), (2 ug/mL), ASC, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse IgG HRP conjugate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0009.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-77297-img0006.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0005.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC/TMS1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419261825.gif)
![Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC/TMS1 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-271120241954153.gif)
![Western Blot: TSLP Antibody [NB110-55233] - Analysis of TSLP in Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NB110-55233-img0004.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: TSLP Antibody [NB110-55233] - Analysis of TSLP in Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/mL")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TSLP Antibody [NB110-55233] - Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/TSLP-Antibody-Western-Blot-NB110-55233-img0002.jpg)
![Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55233] - Figure 3 Immunohistochemistry Validation of TSLP in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nb110-55233_rabbit-polyclonal-tslp-antibody-271120242013252.jpg)
![Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Western-Blot-NB100-65672-img0002.jpg "Western Blot: CD19 Antibody (LE-CD19) [NB100-65672] - analysis of Raji human Lymphoblastic Burkitt's lymphoma whole cell lysate")
![Immunohistochemistry: CD19 Antibody (LE-CD19) [NB100-65672] - Mouse anti human CD19 staining formalin-fixed, paraffin-embedded human tonsil.](http://images.novusbio.com/fullsize/CD19-Antibody-LE-CD19-Immunohistochemistry-NB100-65672-img0001.jpg)