Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76578-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41132-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59072-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77242-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76377-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76789-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76782-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41269-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41305-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76842SS | |||
![Western Blot: ASAH1 Antibody [NBP1-76933] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ASAH1-Antibody-Western-Blot-NBP1-76933-img0005.jpg "Western Blot: ASAH1 Antibody [NBP1-76933] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.")
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-271120242022926.gif)
![Immunocytochemistry/ Immunofluorescence: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-2711202420195360.jpg)
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 u/mL.Red: ASAH1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-2711202420243335.gif)
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-271120242092039.jpg)
![Western Blot: SGK1 Antibody [NBP1-76578] - Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SGK1-Antibody-Western-Blot-NBP1-76578-img0003.jpg "Western Blot: SGK1 Antibody [NBP1-76578] - Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SGK1 Antibody - BSA Free [NBP1-76578] - Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76578_rabbit-polyclonal-sgk1-antibody-271120242074612.jpg)
![Immunocytochemistry/ Immunofluorescence: SGK1 Antibody - BSA Free [NBP1-76578] - Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76578_rabbit-polyclonal-sgk1-antibody-271120241958484.jpg)
![Western Blot: KCNK13 Antibody [NBP2-41132] - Analysis of KCNK13 in rat brain tissue lysate with KCNK13 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/KCNK13-Antibody-Western-Blot-NBP2-41132-img0001.jpg "Western Blot: KCNK13 Antibody [NBP2-41132] - Analysis of KCNK13 in rat brain tissue lysate with KCNK13 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: KCNK13 Antibody - BSA Free [NBP2-41132] - Immunohistochemistry of KCNK13 in human brain tissue with KCNK13 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41132_rabbit-polyclonal-kcnk13-antibody-2711202419541569.jpg)
![Immunocytochemistry/ Immunofluorescence: KCNK13 Antibody - BSA Free [NBP2-41132] - Immunofluorescence of KCNK13 in human brain tissue with KCNK13 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41132_rabbit-polyclonal-kcnk13-antibody-2711202420312920.jpg)
![Western Blot: CIITA Antibody [NBP2-59072] - Analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/CIITA-Antibody-Western-Blot-NBP2-59072-img0006.jpg "Western Blot: CIITA Antibody [NBP2-59072] - Analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: CIITA Antibody [NBP2-59072] - Human brain tissue with CIITA antibody.](http://images.novusbio.com/fullsize/CIITA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59072-img0008.jpg)
![Immunohistochemistry-Paraffin: CIITA Antibody [NBP2-59072] - Human brain tissue. Antibody concentration of 10 ug/ml.](http://images.novusbio.com/fullsize/CIITA-Antibody-Immunohistochemistry-Paraffin-NBP2-59072-img0004.jpg)
![Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Western-Blot-NBP2-41058-img0001.jpg "Western Blot: SARS-CoV-2 Spike Antibody [NBP2-41058] - Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. SARS-CoV-2 Spike(1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells")
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0003.jpg)
![Immunohistochemistry-Paraffin: SARS-CoV-2 Spike Antibody [NBP2-41058] - Immunohistochemistry Validation of SARS-CoV-2 Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 Spike S2 antibody (NBP2-41058, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-Immunohistochemistry-Paraffin-NBP2-41058-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - BSA Free [NBP2-41058] - Figure 14 ELISA TestAntibodies: SARS-CoV-2 (COVID-19) Spike antibody, (1 u/mL). A direct ELISA was performed using immunogen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.5 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp2-41058_rabbit-polyclonal-sars-cov-2-spike-antibody-271120241958462.jpg)
![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 Spike Antibody - BSA Free [NBP2-41058] - Validation of SARS-CoV-2 (COVID-19) Spike in 293T Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike with at 1 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp2-41058_rabbit-polyclonal-sars-cov-2-spike-antibody-271120242022975.jpg)
![Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/PIST-Antibody-Western-Blot-NBP1-77242-img0004.jpg "Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in human testis tissue with PIST antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419531635.gif)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419463455.jpg)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in human testis tissue with PIST antibody at 20 ug/ml.Green: PIST Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202420195363.gif)
![Immunocytochemistry/ Immunofluorescence: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-271120242013170.jpg)
![Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Western-Blot-NBP1-76377-img0005.jpg "Western Blot: IRGM Antibody [NBP1-76377] - Analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: IRGM Antibody [NBP1-76377] - Staining of CD4 in human thymus tissue with CD4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunohistochemistry-Paraffin-NBP1-76377-img0003.jpg)
![Immunohistochemistry: IRGM Antibody [NBP1-76377] - Staining of IRGM in Human Brain tissue with IRGM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunofluorescence-NBP1-76377-img0002.jpg)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420141919.gif)
![Immunohistochemistry: IRGM Antibody - BSA Free [NBP1-76377] - Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76377_rabbit-polyclonal-irgm-antibody-2711202420195388.gif)
![Western Blot: STIM1 Antibody [NBP1-76789] - Mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/STIM1-Antibody-Western-Blot-NBP1-76789-img0001.jpg "Western Blot: STIM1 Antibody [NBP1-76789] - Mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-76789] - Human spleen tissue with Stromal interaction molecule 1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-76789-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NBP1-76789] - Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 u/mL.Red: STIM1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202420285877.gif)
![Immunocytochemistry/ Immunofluorescence: STIM1 Antibody - BSA Free [NBP1-76789] - Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 u/mL.Green: STIM1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202420195326.gif)
![Immunohistochemistry: STIM1 Antibody - BSA Free [NBP1-76789] - Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp1-76789_rabbit-polyclonal-stim1-antibody-2711202419541585.gif)
![Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Western-Blot-NBP1-76782-img0003.jpg "Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-76782] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-76782-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 ug/mL.Red: IRAK3 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-271120242013232.gif)
![Immunohistochemistry: IRAK3 Antibody [NBP1-76782] - Rat Liver tissue with IRAK-M antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunofluorescence-NBP1-76782-img0004.jpg)
![Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunohistochemical staining of rat liver tissue using IRAK3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-2711202419541592.jpg)
![Western Blot: Olig2 Antibody [NBP2-41269] - Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Olig2-Antibody-Western-Blot-NBP2-41269-img0001.jpg "Western Blot: Olig2 Antibody [NBP2-41269] - Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: Olig2 Antibody [NBP2-41269] - Staining of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 5 ug/mL.](http://images.novusbio.com/fullsize/Olig2-Antibody-Immunohistochemistry-NBP2-41269-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in mouse brain tissue with Olig2 Antibody at 20 ug/mL.Green: Olig2 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-271120242045633.gif)
![Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in rat brain tissue with Olig2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-2711202420285837.jpg)
![Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269] - Immunohistochemistry of Olig2 in rat brain tissue with Olig2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-2711202419432216.jpg)
![Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/HES5-Antibody-Western-Blot-NBP2-41305-img0001.jpg "Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: HES5 Antibody - BSA Free [NBP2-41305] - Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-2711202420324837.gif)
![Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 ug/mL.Green: HES5 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-271120242011551.gif)
![Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41305_rabbit-polyclonal-hes5-antibody-2711202420392744.jpg)
![Western Blot: RYBP/DEDAF Antibody [NBP1-76842] - Human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with RYBP/DEDAF antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/RYBP-DEDAF-Antibody-Western-Blot-NBP1-76842-img0003.jpg "Western Blot: RYBP/DEDAF Antibody [NBP1-76842] - Human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with RYBP/DEDAF antibody at 1 ug /ml.")
![Immunocytochemistry/ Immunofluorescence: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunofluorescence of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76842_rabbit-polyclonal-rybp-dedaf-antibody-2711202420414575.gif)
![Immunohistochemistry: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunohistochemistry of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76842_rabbit-polyclonal-rybp-dedaf-antibody-2711202420312932.jpg)
![Immunocytochemistry/ Immunofluorescence: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunofluorescence of RYBP/DEDAF in A549 cells with RYBP/DEDAF antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76842_rabbit-polyclonal-rybp-dedaf-antibody-271120241931323.jpg)
![Immunohistochemistry: RYBP/DEDAF Antibody - BSA Free [NBP1-76842] - Immunohistochemistry of RYBP/DEDAF in mouse liver tissue with RYBP/DEDAF antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76842_rabbit-polyclonal-rybp-dedaf-antibody-2711202419261837.gif)