Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB83501-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB97202-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33182 | |||
| IHC-Prediluted | NBP2-48171 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33064 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
![Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Analysis of paraformaldehyde-fixed HeLa, using FABP4 (NBP1-33616) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33616-img0006.jpg "Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Analysis of paraformaldehyde-fixed HeLa, using FABP4 (NBP1-33616) antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-33616] - FABP4 antibody detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. FABP4 stained by FABP4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-33616-img0009.jpg)
![Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Unstimulated and stimulated 3T3-L1 whole cell extracts (20 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-Western-Blot-NBP1-33616-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Paraformaldehyde-fixed mouse ESC D3, using FABP4 (adipocyte) antibody at 1:200 dilution](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33616-img0005.jpg)
![Western Blot: FABP4/A-FABP Antibody [NBP1-33616] -Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33616_rabbit-polyclonal-fabp4-a-fabp-antibody-western-blot-145202492543..jpg)
![Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33616_rabbit-polyclonal-fabp4-a-fabp-antibody-western-blot-145202492735..jpg)
![Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Detection of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-4C3-Western-Blot-NBP2-29413-img0006.jpg "Western Blot: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Detection of VCAM-1 in HDMVE cell lysate using anti-VCAM-1 antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-4C3-Immunohistochemistry-Paraffin-NBP2-29413-img0004.jpg)
![ELISA: VCAM-1/CD106 Antibody (1.4C3) [NBP2-29413] - Analysis using the HRP conjugate of NBP2-29413. Analysis (in-cell) of HDMVE cells using HRP conjugated VCAM-1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-4C3-ELISA-NBP2-29413-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0009.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).")
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0011.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Protein-Array-NBP2-29437-img0010.jpg)
![N/A Neuropilin-1 [HRP]](http://images.novusbio.com/fullsize2/DATA_Neuropilin1_DNRP10_ELISA_777.jpg "N/A Neuropilin-1 [HRP]")
![N/A Neuropilin-1 [HRP]](http://images.novusbio.com/fullsize2/Neuropilin1_DNRP10_ELISA_493.jpg)
![N/A IGFBP-3 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IGFBP3_DY775_ELISA_2221.jpg "N/A IGFBP-3 [Biotin]")
![N/A IL-19 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL19_DY1035_ELISA_1554.jpg "N/A IL-19 [Biotin]")
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![N/A Butyrylcholinesterase/BCHE [HRP]](http://images.novusbio.com/fullsize2/DATA_Butyrylcholinesterase_DBCHE0_ELISA_586.jpg "N/A Butyrylcholinesterase/BCHE [HRP]")
![N/A Butyrylcholinesterase/BCHE [HRP]](http://images.novusbio.com/fullsize2/Butyrylcholinesterase_DBCHE0_ELISA_500.jpg)
![N/A Granzyme A [Biotin]](http://images.novusbio.com/fullsize2/DATA_Granzyme_A_DY290505_ELISA_2414.jpg "N/A Granzyme A [Biotin]")
![N/A IL-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL2_DY502_ELISA_2072.jpg "N/A IL-2 [Biotin]")