Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21679 | |||
| DyLight 488 | NBP1-77702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3345-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB17161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1920-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2281-SP | |||
| Carrier Free | MAB2281-500 | |||
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0011.jpg "Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC-P analysis of FFPE human prostate tissue using AKT1 phospho Thr308 antibody at 20 ug/mL. Left panel: 20X magnification. Right panel: 40X magnification. Heat antigen retrieval in citrate buffer pH 6.2. Secondary antibody: streptavidin-HRP at 10 ug/mL. Antibody as precipitated red signal with hemotoxylin purple nuclear counterstain. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0014.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC analysis. Detection of phosphorylated AKT phospho Thr308 in human FFPE brain cerebellum tissue (40X). AKT1 phospho Thr308 antibody was used at 20 ug/mL. The image shows strong staining of Purkinje neurons. Red: AKT1 pT308. Purple: Hematoxylin nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunohistochemistry-Paraffin-NBP1-69924-img0003.jpg)
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - WB analysis using the Biotin conjugate of AKT1 pT308 antibody. Detection of Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 inactive recombinant protein. Load: 25 ng per lane. AKT pT308 Biotin conjugated antibody at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ICC/IF analysis using the DyLight 488 conjugate of AKT1 pT308, NBP1-69924. Histone detection using DyLight 488 conjugate (green). Anti-Tubulin was detected using DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0006.jpg)
![ELISA: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ELISA of AKT1 phospho Thr308 Biotin conjugated antibody. Antigen: Unconjugated AKT1 phospho Thr308 and AKT1 non-phospho Thr308. Coating amount: 0.1 ug per well. Primary antibody: AKT1 phospho Thr308 Biotin conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL AKT1 phospho Thr308 Biotin conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-ELISA-NBP1-69924-img0013.jpg)
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL7_M7000_ELISA_937.jpg "N/A IL-7 [HRP]")
![N/A IL-7 [HRP]](http://images.novusbio.com/fullsize2/IL-7_M7000_ELISA_238.jpg)
![N/A CD25/IL-2R alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_CD25_DY223_ELISA_1792.jpg "N/A CD25/IL-2R alpha [Biotin]")
![N/A Midkine [Biotin]](http://images.novusbio.com/fullsize2/DATA_Midkine_DY258_ELISA_1850.jpg "N/A Midkine [Biotin]")
![N/A Nesfatin-1/Nucleobindin-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Nesfatin1_DY5949_ELISA_2145.jpg "N/A Nesfatin-1/Nucleobindin-2 [Biotin]")
![N/A CXCL3/GRO gamma/CINC-2/DCIP-1 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL3_RCN200_ELISA_1086.jpg "N/A CXCL3/GRO gamma/CINC-2/DCIP-1 [HRP]")
![N/A CXCL3/GRO gamma/CINC-2/DCIP-1 [HRP]](http://images.novusbio.com/fullsize2/CXCL3_RCN200_ELISA_351.jpg)
![N/A Fas/TNFRSF6/CD95 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Fas_DY326_ELISA_1929.jpg "N/A Fas/TNFRSF6/CD95 [Biotin]")
![N/A Clusterin/APOJ [HRP]](http://images.novusbio.com/fullsize2/DATA_Clusterin_MCLU00_ELISA_957.jpg "N/A Clusterin/APOJ [HRP]")
![N/A Clusterin/APOJ [HRP]](http://images.novusbio.com/fullsize2/Clusterin_MCLU00_ELISA_253.jpg)