| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83186-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38276-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99700-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35194-20ul | |||
![Western Blot: SMYD2 Antibody [NBP2-20429] - Sample (30 ug of whole cell lysate) A: Hela B: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Western-Blot-NBP2-20429-img0001.jpg "Western Blot: SMYD2 Antibody [NBP2-20429] - Sample (30 ug of whole cell lysate) A: Hela B: HCT116 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SMYD2 Antibody [NBP2-20429] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20429-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SMYD2 Antibody [NBP1-83186] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83186-img0005.jpg "Immunocytochemistry/Immunofluorescence: SMYD2 Antibody [NBP1-83186] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria. Antibody staining is shown in green.")
![Western Blot: SMYD2 Antibody [NBP1-50043] - Detection of Human and Mouse SMYD2 by Western Blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody NBP1-50043 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Western-Blot-NBP1-50043-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMYD2 Antibody [NBP1-50043] - Detection of Human and Mouse SMYD2 by Western Blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody NBP1-50043 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SMYD2 Antibody [NBP1-50043] - Detection of Human and Mouse SMYD2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SMYD2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SMYD2 was also immunoprecipitated by rabbit anti-SMYD2 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SMYD2, the ReliaBLOT (restricted) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/SMYD2-Antibody-Immunoprecipitation-NBP1-50043-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMYD2 Antibody [NBP1-50043] - Detection of human SMYD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMYD2 antibody NBP1-50043 (lot NBP1-50043-2) used for IP at 6 ug per reaction. SMYD2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-50043-1) and another rabbit anti-SMYD2 antibody. For blotting immunoprecipitated SMYD2, NBP1-50043 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Immunoprecipitation-NBP1-50043-img0006.jpg)
![Western Blot: SMYD2 Antibody [NBP3-38276] - Western blot analysis of lysates from Hep G2 cells, using SMYD2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-38276_rabbit-smyd2-pab-10122024120612.jpg "Western Blot: SMYD2 Antibody [NBP3-38276] - Western blot analysis of lysates from Hep G2 cells, using SMYD2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Immunocytochemistry/ Immunofluorescence: SMYD2 Antibody [NBP3-38276] - Immunofluorescence analysis of U2OS cells using SMYD2 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38276_rabbit-smyd2-pab-101220241224119.jpg)
![Western Blot: SMYD2 Overexpression Lysate (Adult Normal) [NBL1-16261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMYD2.](http://images.novusbio.com/fullsize/SMYD2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16261-img0002.jpg "Western Blot: SMYD2 Overexpression Lysate (Adult Normal) [NBL1-16261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMYD2.")
![Chromatin Immunoprecipitation: SMYD2 Antibody [NBP3-09280] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.](http://images.novusbio.com/fullsize/SMYD2-Antibody-Chromatin-Immunoprecipitation-NBP3-09280-img0001.jpg "<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: SMYD2 Antibody [NBP3-09280] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.")
![Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-2120249371584.jpg "Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-212024937496.jpg)
![Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-2120249422645.jpg)
![Immunohistochemistry-Paraffin: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-2120249351312.jpg)
![Western Blot: SMYD3 Antibody (HL2464) - Azide and BSA Free [NBP3-25688] - Whole zebrafish extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-2120249371597.jpg)
![SMYD3 antibody [HL2464] detects SMYD3 protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMYD3 stained by SMYD3 antibody [HL2464] (NBP3-25688) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25688_rabbit-smyd3-mab-hl2464-azide-and-bsa-free-immunocytochemistry-immunofluorescence-141120251828114.jpg)
![Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP3-35194] - Immunofluorescence analysis of L929 cells using SMYD3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35194_rabbit-smyd3-pab-10122024121096.jpg "Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP3-35194] - Immunofluorescence analysis of L929 cells using SMYD3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP3-35194] - Immunofluorescence analysis of C6 cells using SMYD3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35194_rabbit-smyd3-pab-1012202412069.jpg)
![Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP3-35194] - Immunofluorescence analysis of HeLa cells using SMYD3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35194_rabbit-smyd3-pab-101220241243985.jpg)
![Immunohistochemistry: SMYD3 Antibody [NBP3-35194] - Immunohistochemistry analysis of paraffin-embedded Human colon using SMYD3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35194_rabbit-smyd3-pab-101220241224164.jpg)
![Western Blot: SMYD3 Antibody [NBP3-35194] - Western blot analysis of various lysates using SMYD3 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35194_rabbit-smyd3-pab-101220241155282.jpg)
![SMYD3 Antibody [NBP2-20430] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMYD3-Antibody-NBP2-20430-img0011.jpg "SMYD3 Antibody [NBP2-20430] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: SMYD3 Antibody [NBP2-20430] - SMYD3 antibody detects SMYD3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (NBP2-20430) diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp2-20430_rabbit-polyclonal-smyd3-antibody-9102024205645.jpg)
![Western Blot: SMYD3 Antibody [NBP2-20430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (NBP2-20430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20430_rabbit-polyclonal-smyd3-antibody-91020242091471.jpg)
![SMYD3 antibody detects SMYD3 protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMYD3 stained by SMYD3 antibody (NBP2-20430) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20430_rabbit-polyclonal-smyd3-antibody-immunocytochemistry-immunofluorescence-261120251819366.jpg)
