Product Search for cd42b gpib alpha: Novus Biologicals

NBP1-28457

CD42b/GPIb alpha Antibody (MM2/174) - Azide and BSA Free

(1 Review)

0.1 mg

NBP1-28457

Flow Cytometry: CD42b/GPIb alpha Antibody (MM2/174) [NBP1-28457] - Analysis using the PE conjugate of NBP1-28457. Staining of platelets.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NBP1-28502
FITC	NBP1-28501
PE	NBP1-28503

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NB500-511

CD42b/GPIb alpha Antibody (HIP1) - BSA Free

(2 Publications)

0.1 mg

NB500-511

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) purified / GAM-APC.

Surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-511AF350
Alexa Fluor 405	NB500-511AF405
Alexa Fluor 488	NB500-511AF488
Alexa Fluor 532	NB500-511AF532
Alexa Fluor 594	NB500-511AF594
Alexa Fluor 647	NB500-511AF647
Alexa Fluor 700	NB500-511AF700
Alexa Fluor 750	NB500-511AF750
Alexa Fluor Plus 405	NB500-511AFP405
Alexa Fluor Plus 488	NB500-511AFP488
Alexa Fluor Plus 555	NB500-511AFP555
Alexa Fluor Plus 594	NB500-511AFP594
Alexa Fluor Plus 647	NB500-511AFP647
Alexa Fluor Plus 680	NB500-511AFP680
Allophycocyanin	NB500-511APC
Allophycocyanin/Cy7	NB500-511APCCY7
Biotin	NB500-511B
CoraFluor 1	NB500-511CL1
DyLight 350	NB500-511UV
DyLight 405	NB500-511V
DyLight 488	NB500-511G
DyLight 550	NB500-511R
DyLight 594	NB500-511DL594
DyLight 650	NB500-511C
DyLight 680	NB500-511FR
DyLight 755	NB500-511IR
FITC	NB500-511F
HRP	NB500-511H
Janelia Fluor 525	NB500-511JF525
Janelia Fluor 549	NB500-511JF549
Janelia Fluor 646	NB500-511JF646
Janelia Fluor 669	NB500-511JF669
PE	NB500-511PE
PE/Atto594	NB500-511PEATT594
PE/Cy5.5	NB500-511PECY55
PE/Cy7	NB500-511PECY7
PerCP	NB500-511PCP
mFluor Violet 450 SE	NB500-511MFV450
mFluor Violet 500 SE	NB500-511MFV500
mFluor Violet 610 SE	NB500-511MFV610

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AF4067

CD42b/GPIb alpha Antibody [Unconjugated]

(3 Publications)

100 μg

AF4067

HAF016). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD42b/GPIb a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD42b/GPIb a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A)." class="big_lightbox">

Human whole blood CD41<SUP>+</SUP>platelets were stained with Sheep Anti-Human CD42b/GPIb a Polyclonal Antibody (Catalog # AF4067) followed by NL637-conjugated anti sheep antibody (Catalog # NL011) and Human CD41 FITC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # <a class=

5-001-A)." class="big_thumb" />

(A) Western blot analysis of Bar-III on recombinant vWF-A1 domain (rvWF-A1). vWF-A1 (5 µg) was treated with Bar-III (4 µg) for 60 min at 37 °C, as described in methodology section. The proteolysis of vWF-A1 (indicated by an arrow) was analyzed using SDS-PAGE (7–15% gradient gel) and blotted with mice anti-vWF IgG. (B) Bar-III does not cleave the vWF receptor partner GPIb alpha on platelets. Washed platelets were incubated with Bar-III (4 µg) at 37 °C at the indicated intervals, and the reactions were terminated by addition of SDS loading sample buffer. The platelet lysate was probed in WB with anti-CD42/GPIb. Note the intact ~130-kDa GPIb alpha expression on platelets. (C) Platelet pellets or supernatants of WPs treated with Bar-III (4 µg) for 60 min at 37 °C. The positions of GPVI (~62-kDa) and the soluble GPVI fragment (~55-kDa) are indicated. C, platelet control. These results are representative of at least three similar experiments for each item. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39591241), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4067U-100UG
Alexa Fluor 405	AF4067V-100UG
Alexa Fluor 488	AF4067G-100UG
Alexa Fluor 532	AF4067X-100UG
Alexa Fluor 594	AF4067T-100UG
Alexa Fluor 647	AF4067R-100UG
Alexa Fluor 700	AF4067N-100UG
Alexa Fluor 750	AF4067S-100UG
Alexa Fluor Plus 405	AF4067AFP405-100UG
Alexa Fluor Plus 488	AF4067AFP488-100UG
Alexa Fluor Plus 555	AF4067AFP555-100UG
Alexa Fluor Plus 594	AF4067AFP594-100UG
Alexa Fluor Plus 647	AF4067AFP647-100UG
Alexa Fluor Plus 680	AF4067AFP680-100UG
Allophycocyanin	AF4067A
Biotin	AF4067B
CoraFluor 1	AF4067CL1
DyLight 350	AF4067D
DyLight 405	AF4067E
DyLight 488	AF4067K
DyLight 550	AF4067L
DyLight 594	AF4067M
DyLight 650	AF4067W
DyLight 680	AF4067Y
DyLight 755	AF4067Z
FITC	AF4067F
HRP	AF4067H
Janelia Fluor 525	AF4067JF525
Janelia Fluor 549	AF4067I
Janelia Fluor 646	AF4067J
Janelia Fluor 669	AF4067JF669
PE	AF4067P
PerCP	AF4067C
mFluor Violet 450 SE	AF4067MFV450
mFluor Violet 500 SE	AF4067MFV500
mFluor Violet 610 SE	AF4067MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4067-SP

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NB100-63790

CD42b/GPIb alpha Antibody (AK2) - BSA Free

(2 Publications)

0.1 mg

NB100-63790

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-63790AF350
Alexa Fluor 405	NB100-63790AF405
Alexa Fluor 488	NB100-63790AF488
Alexa Fluor 532	NB100-63790AF532
Alexa Fluor 594	NB100-63790AF594
Alexa Fluor 647	NB100-63790AF647
Alexa Fluor 700	NB100-63790AF700
Alexa Fluor 750	NB100-63790AF750
Alexa Fluor Plus 405	NB100-63790AFP405
Alexa Fluor Plus 488	NB100-63790AFP488
Alexa Fluor Plus 555	NB100-63790AFP555
Alexa Fluor Plus 594	NB100-63790AFP594
Alexa Fluor Plus 647	NB100-63790AFP647
Alexa Fluor Plus 680	NB100-63790AFP680
Allophycocyanin	NB100-63790APC
Allophycocyanin/Cy7	NB100-63790APCCY7
Biotin	NB100-63790B
CoraFluor 1	NB100-63790CL1
DyLight 350	NB100-63790UV
DyLight 405	NB100-63790V
DyLight 488	NB100-63790G
DyLight 550	NB100-63790R
DyLight 594	NB100-63790DL594
DyLight 650	NB100-63790C
DyLight 680	NB100-63790FR
DyLight 755	NB100-63790IR
FITC	NB100-63790F
HRP	NB100-63790H
Janelia Fluor 525	NB100-63790JF525
Janelia Fluor 549	NB100-63790JF549
Janelia Fluor 646	NB100-63790JF646
Janelia Fluor 669	NB100-63790JF669
PE	NB100-63790PE
PE/Atto594	NB100-63790PEATT594
PE/Cy5.5	NB100-63790PECY55
PE/Cy7	NB100-63790PECY7
PerCP	NB100-63790PCP
mFluor Violet 450 SE	NB100-63790MFV450
mFluor Violet 500 SE	NB100-63790MFV500
mFluor Violet 610 SE	NB100-63790MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-63790-0.025mg

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MAB4067

CD42b/GPIb alpha Antibody (486805) [Unconjugated]

(2 Publications)

100 μg

MAB4067

HAF018). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD42b/GPIb a at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

FAB7616P) and either (A) Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human CD41<sup>+</sup>platelets were stained with Mouse Anti-Human Integrin a2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # <a class=

FAB7616P) and either (A) Mouse Anti-Human CD42b/GPIb a Monoclonal Antibody (Catalog # MAB4067) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Membrane fusion study and protein markers characterization. (A) Fluorescence intensity of P-EVs in the FRET study and quantification of FRET efficiency after membrane fusion at different ratio. PMVs labeled with a pair of FRET fluorescent dyes were fused with increasing amounts of EVs. A recovery of fluorescence at 534 nm indicated the fusion of PMVs and EVs (PMV:EV = the protein ratio of platelet membrane to EV). (B) Structured illumination microscopic imaging of either P-EVs or a mixture of PMVs and EVs without extrusion (green: PMV, red: EV). Scale bar = 5 µm. (C) Immunogold TEM images of PMVs, EVs and P-EVs probe for CD90 (green arrows, small gold nanoparticles) and GPIb alpha (red arrow, large gold nanoparticles). Scale bar = 100 nm. (D) Protein contents of EVs, P-EVs and PMVs on a Coomassie blue stained SDS-PAGE gel. (E) Western blot analysis of Alix, TSG101, CD9 and CD90 expression in EVs, P-EVs and PMVs. (F) Western blot analysis of GP Ib alpha , integrin alpha 2/ beta 1 and GPIIbIIIa expression in EVs, P-EVs and PMVs. All samples were run at equivalent protein concentrations. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33664870), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4067U-100UG
Alexa Fluor 405	FAB4067V-100UG
Alexa Fluor 488	FAB4067G-100UG
Alexa Fluor 532	FAB4067X
Alexa Fluor 594	FAB4067T-100UG
Alexa Fluor 647	FAB4067R-100UG
Alexa Fluor 700	FAB4067N-100UG
Alexa Fluor 750	FAB4067S-100UG
Alexa Fluor Plus 405	FAB4067AFP405-100UG
Alexa Fluor Plus 488	FAB4067AFP488-100UG
Alexa Fluor Plus 555	FAB4067AFP555-100UG
Alexa Fluor Plus 594	FAB4067AFP594-100UG
Alexa Fluor Plus 647	FAB4067AFP647-100UG
Alexa Fluor Plus 680	FAB4067AFP680-100UG
Allophycocyanin	FAB4067A
Allophycocyanin/Cy7	FAB4067APCCY7
Biotin	FAB4067B
CoraFluor 1	FAB4067CL1
DyLight 350	FAB4067D
DyLight 405	FAB4067E
DyLight 488	FAB4067K
DyLight 550	FAB4067L
DyLight 594	FAB4067M
DyLight 650	FAB4067W
DyLight 680	FAB4067Y
DyLight 755	FAB4067Z
FITC	FAB4067F
HRP	FAB4067H
Janelia Fluor 525	FAB4067JF525
Janelia Fluor 549	FAB4067I
Janelia Fluor 646	FAB4067J
Janelia Fluor 669	FAB4067JF669
PE/Atto594	FAB4067PEATT594
PE/Cy5.5	FAB4067PECY55
PE/Cy7	FAB4067PECY7
PerCP	FAB4067C
mFluor Violet 450 SE	FAB4067MFV450
mFluor Violet 500 SE	FAB4067MFV500
mFluor Violet 610 SE	FAB4067MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB4067A
Carrier Free	MAB4067-SP
Carrier Free	FAB4067V-100UG
Carrier Free	FAB4067U-100UG
Carrier Free	FAB4067S-100UG
Carrier Free	FAB4067R-100UG
Carrier Free	FAB4067N-100UG
Carrier Free	FAB4067G-100UG
Carrier Free	FAB4067T-100UG

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4067-GP

Recombinant Human CD42b/GPIb alpha Protein, CF

(1 Publication)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-85749

CD42b/GPIb alpha Antibody - BSA Free

0.1 ml

NBP1-85749

Staining of human bone marrow shows moderate positivity in megakaryocytes.

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.

Staining of human fallopian tube shows moderate positivity in cilia in glandular cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85749-25ul

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8428-GP

Recombinant Mouse CD42b/GPIb alpha Protein, CF

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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H00002811-M02

CD42b/GPIb alpha Antibody (1C6) - Azide and BSA Free

(1 Publication)

0.1 mg

H00002811-M02

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-75153

Human CD42b/GPIb alpha ELISA Kit (Colorimetric)

1 Kit

NBP2-75153

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

3.13 - 200 ng/mL

Sensitivity:

1.88 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP3-21781

CD42b/GPIb alpha Antibody (SR1163) - BSA Free

100 ul

NBP3-21781

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21781-50ul

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NBP2-11152

CD42b/GPIb alpha Overexpression Lysate

0.1 mg

NBP2-11152

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

CD42b/GPIb alpha Antibody (MM2/174) - Azide and BSA Free

CD42b/GPIb alpha Antibody (HIP1) - BSA Free

CD42b/GPIb alpha Antibody [Unconjugated]

CD42b/GPIb alpha Antibody (AK2) - BSA Free

CD42b/GPIb alpha Antibody (486805) [Unconjugated]

Recombinant Human CD42b/GPIb alpha Protein, CF

CD42b/GPIb alpha Antibody - BSA Free

Recombinant Mouse CD42b/GPIb alpha Protein, CF

CD42b/GPIb alpha Antibody (1C6) - Azide and BSA Free

Human CD42b/GPIb alpha ELISA Kit (Colorimetric)

CD42b/GPIb alpha Antibody (SR1163) - BSA Free

CD42b/GPIb alpha Overexpression Lysate