SM047: A Marker for Ovarian Adenocarcinoma

Mon, 09/30/2013 - 12:13


Glandular tissues synthesise substances for release, such as hormones and enzymes. The term adenocarcinoma is used to describe a cancer that originates within a glandular tissue, for example the ovary.

The incidence of ovarian cancer is low, yet ovarian cancer is the most lethal gynaecological malignancy and typically has a poor prognosis. There are currently known to be more than 30 different types of ovarian cancer, of which approximately 90% originate from epithelial cells. To date, the most well-established biomarker for the diagnosis of ovarian cancer is MUC16, previously known as CA125. However MUC16 is associated with several other pathological conditions and work is on-going to fully elucidate its role in cancer cell growth and disease progression (1). MUC16 is a tumor-associated antigen that is cleaved from the surface of ovarian cancer cells and shed into the blood. The progression of ovarian carcinoma is associated with an increase in serum MUC16 levels, whilst a decline in these levels is linked to a response to therapy (2).

The SM047 antibody is a mouse monoclonal IgM which binds to the glycocalyx (the extracellular glycolipid and glycoprotein material found at the cell surface) of ovarian adenocarcinoma cells. When the SM047 antibody was developed in 2001, it was used to stain a panel of primary ovarian adenocarcinomas, which included serous, endometroid, clear cell and mucinous tumours. Strong membrane staining was seen in the serous, endometroid and clear cell carcinomas, whilst ovarian mucinous tumours were the exception and exhibited weak cytoplasmic staining or no staining at all (3).

Immunocytochemistry/Immunofluorescence: SM047 Antibody Immunocytochemistry/Immunofluorescence: SM047 Antibody

A subsequent study in 2003 showed that cellular staining with the SM047 antibody may be of value in the diagnosis of ovarian adenocarcinoma in peritoneal fluids (4). The peritoneum is a double-layered membrane which lines the abdominal wall and covers the organs within the abdominal cavity. It produces a lubricating fluid that allows the abdominal organs to glide smoothly over one another; a build-up of fluid between the peritoneal layers is known as ascites. Ascites is a symptom of many types of cancer, since the metastasis of cancer cells to the lining of the abdomen can irritate the peritoneum and cause fluid to build up. SM047 may be used to detect ovarian adenocarcinoma cells in these fluids, and as such may be used as a marker to tailor treatment following diagnosis.

  1. PMID: 23484144
  2. PMID: 20348949
  3. PMID: 11422498
  4. PMID: 12588308

Novus Biologicals offers SM047 antibodies for your research needs.

 

Written by Emma Easthope


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