Real-time Cell Analysis Throws New Light on GPCR Function

Thu, 07/29/2010 - 08:16

The G-protein coupled receptor (GPCR) family constitutes one of the largest protein families in the mammalian kingdom, with over 800 proteins so far discovered. Our GPCR antibody catalog covers all 6 classes, with antibodies raised against Metabotropic Glutamate/Pheromone receptors; Rhodopsin-like receptors; Secretin receptors; Fungal Mating Pheromone receptors; cAMP receptors and Frizzled/Smoothened receptors. The majority are of the Class A (Rhodopsin-like) class.

GPCRs are a major focus for antibody suppliers. They are fundamental to many life processes, enabling cells to react to changes in their environment by activating intracellular signalling pathways (generally the cAMP or phosphatidylinositol signal transduction pathways), or by binding extracellular molecules (ligands.) Ligands include hormones, odors, neurotransmitters and light-sensitive chemicals.

Immunocytochemistry/Immunofluorescence: 5-oxo-ETE GPCR Antibody Immunocytochemistry/Immunofluorescence: 5-oxo-ETE GPCR Antibody

GPCRs are involved in most sensory systems, and can affect behavior and moods by interaction with chemicals like dopamine and serotonin. In vivo studies have shown that modulation of GPCR activity can have a therapeutic effect in areas as diverse as neurology, immunology, cardiology, and oncology. We at Novus Biologicals provide GPCR antibodies for research into a wide range of clinical and physiological areas.

Scientists are constantly looking for ways to improve their antibodyprotocols, in terms of reagents, methodology and equipment. Recently, a new real-time cell analysis method using impedance-based signals (the Roche xCELLigence System) was used to assay a wide range of GPCRs in primary and tumour cells.

Unlike traditional assays using engineered cell lines, the RTCA method allowed multiple proteins to be studied simultaneously, in a near-native environment at normal expression levels. It can be used with both labelled and unlabelled antibodyreagents, and represents a great breakthrough in biomolecular science.

Novus Biologicals offers many GPCR reagents for your research needs including:



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