PABP: A central regulator of mRNA translation

Mon, 02/02/2015 - 14:33


PABP is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA and is required for poly(A) shortening, translation initiation, and possibly mRNA export and import. In humans, PABPs are small nuclear isoforms within a conserved gene family of at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). PABPs are of special interest because due to their high affinity for A-rich mRNA sequences, they are involved in all mRNA-dependent events. Not surprisingly, they interact with a wide variety of proteins and are important to study because RNA-binding proteins are the foundation for proper mRNA function and organization within the cell.

PABP antibody

Immunocytochemistry/Immunofluorescence: PABP Antibody

The structure, domain organization and activity regulation of the PABPs is nicely summarized by Elisseva et al from the Moscow Institute of Protein Research (1).  Furthermore, Goss' group has published a comprehensive review pulling from years of extensive research on the complexity of the PABP metabolic network, including functional roles and self-interactions (2). They share new insights on specific roles of different PABP family members in viral infection and development, including evidence for the exchange of nuclear and cytoplasmic forms and post-translational modifications. It appears the system exhibits a high degree of fine-tuning. Chazal's group used the PABP antibody to investigate the mechanism behind how the exon junction complex (EJC) core component activates translation; they determined that this particular kind of splicing event requires eIF3 interaction and subsequent stimulation of enhanced cellular translation (3).

Novus Biologicals offers PABP reagents for your research needs including:

PMIDs

  1. 24490729
  2. 23424172
  3. 23530232

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