Most commercially available antibodies produced with traditional immunization technology, using peptides or other immunogens isolated and purified from cell lysates.
Typically, mice are immunized with the antigen in groups, varying the mouse strain and antigen dosage. Pre-immune and immune sera are evaluated and mice showing the best immune response are selected for somatic cell fusion. This is the fusion of antibody-producing lymphocytes with proprietary myeloma cells from a non-antibody producing source, creating a hybridoma.
The hybridoma cells then proliferate, producing a continuous supply of monoclonal antibodies. Hybridomas producing the best performing Abs are then cloned, increasing the likelihood of monoclonality. Although it is the standard technique for monoclonal antibodyproduction, it often results in a poor yield when difficult target antigens are used. Problematic immunogens include transmembrane, conserved, and smaller sized proteins.
Immunocytochemistry/Immunofluorescence: GAT1 Antibody
Recently, Novus Biologicals introduced a totally new concept to our antibody database: genomic antibody technology, or GAT. Using this technique, immunoglobulins can be targeted to the most complex of immunogens.
Genomic Antibody Technology avoids the use of native-sourced antigen entirely. Instead, the amino acid sequence is used, supplied as electronic information. The sequence is biosynthesized and placed in a proprietary plasmid vector (DNA able to exist outside the chromosome.) The plasmid is injected into specific tissues of a host animal (i.e. transfection). The cells take up the vector and proceed to synthesize and secrete the immunogen, which is immediately recognized by the immune system. The result is production of highly specific monoclonal antibodies in response.
GAT antibodies are an exciting new addition to our antibody catalog, solving many of the problems associated with hybridoma technology and streamlining the whole process.
Novus Biologicals offers many GAT reagents for your research needs including: