Blue Marker Antibody: An easy solution for BLUE-pre-stained markers

Mon, 07/07/2014 - 13:11

Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies. It was first described by Towbin, in 1979 and has since become one of the most commonly used methods in life science research. In Western blotting, a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. The proteins are visualized with antibodies specific to the target under investigation. Typically pre-stained molecular weight markers are transferred alongside the target proteins enabling on-blot molecular weight sizing of the protein. However pre-stained markers are not visualized by the antibody detection methods making sizing more difficult and impractical. Novus Anti-Blue Antibody (NBP2-33376) detects BLUE-pre-stained molecular weight markers, does not cross-react with whole cell proteins of different species, and does not interfere with the detection by other antibodies. It is compatible with any pre-stained marker (blue) and can be used in conjunction with any primary antibody. It can be detected by any secondary anti-mouse antibody.

Western Blot: Blue Marker Antibody Western Blot: Blue Marker Antibody


It eliminates manual marking of your protein marker bands and guessing the accuracy. The anti-Blue antibody is able to visualize your markers and your target proteins together on the same blot, in the same exposure. It is very easy to use and fits in with existing work flows. The anti-Blue antibody has been tested with whole cell lysates of different species, confirming the high specificity towards blue-stained proteins. No cross-reactivity with whole cell proteins has been detected.

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