Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies. It was first described by Towbin, et.al in 1979 and has since become one of the most commonly used methods in life science research. In Western blotting, a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. The proteins are visualized with antibodies specific to the target under investigation.
