| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88584-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05105-20ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 532 | NBP3-45958AF532 | |||
| Alexa Fluor 700 | NBP3-45958AF700 | |||
| Allophycocyanin | NBP3-45958APC | |||
| DyLight 755 | NBP3-45958IR | |||
| PE | NBP3-45958PE | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72710 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03652-20ul | |||
![Immunocytochemistry/Immunofluorescence: MAP4K2 Antibody [NBP1-88584] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88584-img0004.jpg "Immunocytochemistry/Immunofluorescence: MAP4K2 Antibody [NBP1-88584] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining in human lymph node and liver tissues using anti-MAP4K2 antibody. Corresponding MAP4K2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Immunohistochemistry-Paraffin-NBP1-88584-img0007.jpg)
![Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Immunohistochemistry-Paraffin-NBP1-88584-img0005.jpg)
![Immunohistochemistry-Paraffin: MAP4K2 Antibody [NBP1-88584] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Immunohistochemistry-Paraffin-NBP1-88584-img0006.jpg)
![Western Blot: MAP4K2 Antibody [NBP3-05105] - Analysis of extracts of various cell lines, using MAP4K2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Western-Blot-NBP3-05105-img0001.jpg "Western Blot: MAP4K2 Antibody [NBP3-05105] - Analysis of extracts of various cell lines, using MAP4K2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: MAP4K2 Antibody - BSA Free [NBP3-45958] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (MAP4K2 antibody) at dilution of 1:300](http://images.novusbio.com/fullsize/nbp3-45958_rabbit-map4k2-pab-bsa-free-284202511492210.jpg "Western Blot: MAP4K2 Antibody - BSA Free [NBP3-45958] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (MAP4K2 antibody) at dilution of 1:300")
![Western Blot: MAP4K2 Antibody [NBP1-58852] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Western-Blot-NBP1-58852-img0003.jpg "Western Blot: MAP4K2 Antibody [NBP1-58852] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.")
![Western Blot: MAP4K2 Antibody [NBP1-58851] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Western-Blot-NBP1-58851-img0003.jpg "Western Blot: MAP4K2 Antibody [NBP1-58851] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Western Blot: MAP4K2 Overexpression Lysate (Adult Normal) [NBP2-07852] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP4K2.](http://images.novusbio.com/fullsize/MAP4K2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07852-img0001.jpg "Western Blot: MAP4K2 Overexpression Lysate (Adult Normal) [NBP2-07852] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP4K2.")
![Western Blot: MAP4K2 Antibody [H00005871-B01P] - Analysis of MAP4K2 expression in transfected 293T cell line by MAP4K2 polyclonal antibody. Lane 1: MAP4K2 transfected lysate(90.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MAP4K2-Antibody-Western-Blot-H00005871-B01P-img0001.jpg "Western Blot: MAP4K2 Antibody [H00005871-B01P] - Analysis of MAP4K2 expression in transfected 293T cell line by MAP4K2 polyclonal antibody. Lane 1: MAP4K2 transfected lysate(90.2 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)
![Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Western-Blot-NBP3-03652-img0002.jpg "Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![<b>Genetic Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Knockout-Validated-NBP3-03652-img0001.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251636328.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516345226.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of lysates from A-549 cells, using MKK4/MEK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516293950.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251633412.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Rat lung using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251640210.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of various lysates, using MKK4/MEK4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516283264.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516283227.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of lysates from HeLa cells, using MKK4/MEK4 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516345299.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249432880.jpg "Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249403813.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Untransfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249415916.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-212024941452.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249355531.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249422682.jpg)