MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " alt="THP-1 cells were stained with Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB812, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human Flt‑3/Flk‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " alt="M1 cells were stained with Rat Anti-Mouse Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # mab7681, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF008). Specific bands were detected for Phospho-Flt-3/Flk-2 (Y591) at approximately 160 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). Specific bands were detected for Phospho-Flt-3/Flk-2 (Y591) at approximately 160 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Flt-3/Flk-2 (Y591) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF368), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Flt-3/Flk-2 (Y591) at approximately 160 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " alt="M1 cells were stained with Goat Anti-Mouse Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF768, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human Flt‑3/Flk‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

308-FK) binds with an ED₅₀ of 0.060‑0.720 ng/mL." class="big_lightbox"> 308-FK) binds with an ED₅₀ of 0.060‑0.720 ng/mL." alt="When Biotinylated Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein (Catalog # AVI11064) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human Flt-3 Ligand/FLT3L (308-FK) binds with an ED₅₀ of 0.060‑0.720 ng/mL." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Flt‑3/Flk‑2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB8122) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB050R) coated plate, Recombinant Human Flt-3 Ligand (Catalog # 308-FK) binds with an ED₅₀ of 2-12 ng/mL." class="big_lightbox"> MAB050R) coated plate, Recombinant Human Flt-3 Ligand (Catalog # 308-FK) binds with an ED₅₀ of 2-12 ng/mL." alt="When Recombinant Human Flt-3/Flk-2 His-tag (Catalog # 10284-F3) is captured onto a His Tag Antibody (Catalog # MAB050R) coated plate, Recombinant Human Flt-3 Ligand (Catalog # 308-FK) binds with an ED₅₀ of 2-12 ng/mL." class="big_thumb" />