DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder." alt=" alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human/Mouse/Rat alpha -Smooth Muscle Actin Monoclonal Antibody (Novus Catalog # NBP2-33006) at 0.25ug/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cytoskeleton. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33006-0.2mg | |||
| BSA Free | NBP2-33006-0.02mg | |||
| Azide and BSA Free | NBP2-34522-0.2mg | |||
| Azide and BSA Free | NBP2-34522-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47698-0.1mg | |||
| IHC-Prediluted | NBP2-48132 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47699-0.1mg | |||
| IHC-Prediluted | NBP2-48157 | |||
![Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of Mouse Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NB300-978-img0007.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of Mouse Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978] - SMA antibody staining on mouse fibroblasts. Primary antibody at 1:1000, incubation at 4C overnight. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-978-img0006.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978] - Infarcted mouse heart frozen tissue section immunostained with alpha-Smooth Muscle Actin Antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NB300-978-img0003.jpg)
![alpha-Smooth Muscle Actin Antibody [NB300-978] - (1ug/ml) staining of HeLa (A) and (2ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-NB300-978-img0008.jpg)
![Immunohistochemistry-Paraffin: Goat Polyclonal alpha-Smooth Muscle Actin Antibody [NB300-978] - Analysis of alpha-Smooth Muscle Actin Antibody on mouse heart tissue. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-978_goat-polyclonal-alpha-smooth-muscle-actin-antibody-immunohistochemistry-paraffin-61202594652..jpg)
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # .png)
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-17720241003238.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha Smooth Muscle Actin (1A4/asm-1) Antibody NBP2-34522JF549 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Janelia Fluor 549.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-17720241044610.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - Human tonsil frozen tissue section stained for alpha-Smooth Muscle Actin (red) with the Janelia Fluor 549 conjugate of the Azide and BSA-Free version of alpha-Smooth Muscle Actin Antibody (1A4/asm-1), and counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-17720249362624.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-1772024936267.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - alpha-Smooth Muscle Actin expression in colorectal tumor cells. Using Allophycocyanin conjugated version of antibody (NBP2-34522APC). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-1772024104461.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (1A4/asm-1) NBP2-34522AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-17720241003226.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - Analysis of alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [Alexa Fluor® 647] (Catalog Number: NBP2-34522AF647) using two lung cancer cell lines to acquire the subcutaneous tumors. MMT status of macrophages was then analyzed. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-110202413103111.png)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free [NBP2-33006] - aSMA stained over night in human FFPE tonsil section ((Catalog # NBP2-33006AF750)). Heat mediated antigen retrieval at pH 9. 1:50 Dilution in 3% BSA, incubation over night at 4 °C. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-61202511214025.png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Images demonstrating SMA immunoreactivity in a variety of human FFPE tissue sections. NBP2-33006 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-immunohistochemistry-paraffin-2242025134951..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Images demonstrating SMA immunoreactivity in a variety of canine FFPE tissue sections. NBP2-33006 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-immunohistochemistry-paraffin-2242025135659..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Images demonstrating SMA immunoreactivity in a variety of feline FFPE tissue sections. NBP2-33006 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-immunohistochemistry-paraffin-224202514040..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - SMA in FFPE sections of pig tissue. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-immunohistochemistry-paraffin-224202514429..png)
![Immunohistochemistry-Paraffin: Mouse Monoclonal alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [NBP2-33006] - Images demonstrating SMA immunoreactivity in a variety of horse FFPE tissue sections. NBP2-33006 was used at a concentration of 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 5min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-mouse HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-33006_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-1a4-asm-1-immunohistochemistry-paraffin-152025143957..png)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0011.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.")
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NBP1-30894-img0032.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0023.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0024.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0025.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0026.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0028.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0031.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse smooth muscle.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020241959359.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. <br>Sample: Paraffin-embedded liver carcinoma stroma. <br>alpha smooth muscle Actin antibody (NBP1-30894) diluted at 1:500.<br>Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242011442.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242075975.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041219.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse colon.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205699.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse duodenum.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242063310.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).</br>Sample: Paraffin-embedded human normal colon tissue.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, 20 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420122078.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse smooth muscle.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205673.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse smooth muscle.</br>alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420104449.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041283.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-1610202419414539.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of HepG2 cells using (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunocytochemistry-Immunofluorescence-NBP2-22120-img0008.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis using alpha-Smooth Muscle Actin Antibody (4A4) against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate. The theoretical molecular weight of the antibody is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Western-Blot-NBP2-22120-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using alpha-Smooth Muscle Actin Antibody (4A4) with DAB staining.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Paraffin-NBP2-22120-img0009.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0013.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (4A4) (green) and negative control (purple).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Flow-Cytometry-NBP2-22120-img0007.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0015.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-ELISA-NBP2-22120-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) - BSA Free [NBP2-22120] - Differential effects of the 20(R/S)‐Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co‐immunofluorescence staining of the aortic root for VSMCs (anti‐ alpha ‐SMA antibody, red) & proliferation marker PCNA (green) & a bar graph summarizing the results (n = 3). Scale bar: 20 μm. B, Western blot analysis of cyclin D1, cyclin E & PCNA protein expression within plaques. The bands are quantified by densitometric analysis. Protein expression of cyclin D1 & PCNA was normalized to beta ‐actin, & expression of cyclin E was normalized to GADPH (n = 3, 4, & 4 for cyclin D1, cyclin E & PCNA, respectively). The results are expressed as the mean values ± SEM. *P < .05, **P < .01, ***P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29566305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22120_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-4a4-310202415395945.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44463-img0008.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Immunofluorescence Analysis of HeLa cells labeled with alpha-Smooth Muscle Actin Antibody (ACTA/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Rat Lung stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Intracellular-NBP2-44463-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Colon Carcinoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - Human Leiomyosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44463-img0003.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (ACTA2/791) [NBP2-44463] - An intracellular stain was performed on HepG2 cells with alpha-Smooth Muscle Actin Antibody (ACTA2/791) NBP2-47698PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-ACTA2-791-Flow-Cytometry-NBP2-44463-img0006.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/Mouse-alpha-Smooth-Muscle-Actin-ELISA-Kit-Colorimetric-ELISA-NBP2-66429-img0002.jpg "ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.")
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - DRC image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-166202313361..jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-137202316216.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Samples were spiked with high concentrations of Mouse alpha-Smooth Muscle Actin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-132202416225244.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-4320249133249.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.Lane 1: Hela-si NT cell lysate (10 ug/Lane)Lane 2: Hela-si alpha smooth muscle Actin cell lysate (10 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel. was shown to specifically react with alpha smooth muscle Actin in Hela-si NT cells. Weakened band was observed when Hela-si alpha smooth muscle Actin sample was tested. Hela-si NT and Hela-si alpha smooth muscle Actin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67440_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-sy02-64-western-blot-2372024113558..jpg)
![Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE IMR-90 cells by ICC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunocytochemistry-NBP2-78836-img0002.jpg "Immunocytochemistry: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE IMR-90 cells by ICC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of human alpha-Smooth Muscle Actin in FFPE breast by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunohistochemistry-NBP2-78836-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] - Detection of mouse alpha-Smooth Muscle Actin in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-alpha Smooth Muscle Actin recombinant monoclonal [BLR082G] (NBP2-78836). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-BLR082G-Immunohistochemistry-NBP2-78836-img0003.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -: Whole cell lysate (50 ug) fromJurkat, HeLa, IMR-90, HEK293T, and Hep-G2 cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-alphaSmooth Muscle Actin recombinant monoclonal antibody[BLR082G] used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-western-blot-213202412531..jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -Whole cell lysate (10 ug) from CT26, NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti alpha Smooth Muscle Actin recombinant monoclonal antibody [BLR082G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-western-blot-2132024125440..jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (BLR082G) [NBP2-78836] -: Rabbit anti-alpha SmoothMuscle Actin recombinant monoclonal antibody [BLR082G]. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp2-78836_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-blr082g-immunohistochemistry-paraffin-213202412568..jpg)
![ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/Rat-alpha-Smooth-Muscle-Actin-ELISA-Kit-Colorimetric-ELISA-NBP2-66430-img0002.jpg "ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Standard Curve. Image from verified customer review.")
![ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66430_rat-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-1662023132934..jpg)
![ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66430_rat-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-1372023161810..jpg)
![ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Samples were spiked with high concentrations of Rat alpha-Smooth Muscle Actin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66430_rat-alpha-smooth-muscle-actin-elisa-kit-colorimetric-13220241692228.jpg)
![ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66430_rat-alpha-smooth-muscle-actin-elisa-kit-colorimetric-432024857417.jpg)
![SDS-Page: Recombinant Human, alpha-Smooth Muscle Actin Protein [H00000059-P01] - Recombinant Human alpha-Smooth Muscle Actin Protein [H00000059-P01] - 12.5% SDS-PAGE stained with Coomassie Blue. The band indicates an observed molecular weight of ~68 kDa.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Smooth-Muscle-Actin-Protein-SDS-Page-H00000059-P01-img0003.jpg "SDS-Page: Recombinant Human, alpha-Smooth Muscle Actin Protein [H00000059-P01] - Recombinant Human alpha-Smooth Muscle Actin Protein [H00000059-P01] - 12.5% SDS-PAGE stained with Coomassie Blue. The band indicates an observed molecular weight of ~68 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunocytochemistry-Immunofluorescence-NBP2-44464-img0011.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Human Angiosarcoma stained with alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (1A4 + ACTA2/791) [NBP2-44464] - Paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-1A4-+-ACTA2-791-Immunohistochemistry-Paraffin-NBP2-44464-img0009.jpg)
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