| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18686-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03809-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97642-200ul | |||
| BSA Free | NBP2-97642-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98822-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35870-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35620-20ul | |||
![Western Blot: MURF1 Antibody [NBP2-11061] - WB of antibody with sample. Antibody dilution 1:500 in Dilution Buffer. MW of MURF1 is 41 and 52 kDa.](http://images.novusbio.com/fullsize/MURF1-Antibody-Western-Blot-NBP2-11061-img0001.jpg "Western Blot: MURF1 Antibody [NBP2-11061] - WB of antibody with sample. Antibody dilution 1:500 in Dilution Buffer. MW of MURF1 is 41 and 52 kDa.")
![Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP3-14694-img0001.jpg "Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.")
![Western Blot: cIAP-2/HIAP-1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-70184] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3.](http://images.novusbio.com/fullsize/cIAP-2-HIAP-1-Antibody-OTI1A9-Azide-and-BSA-Free-Western-Blot-NBP2-70184-img0001.jpg "Western Blot: cIAP-2/HIAP-1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-70184] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3.")
![Flow Cytometry: cIAP-2/HIAP-1 Antibody (OTI1A9) - Azide and BSA Free [NBP2-70184] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by BIRC3 antibody.](http://images.novusbio.com/fullsize/cIAP-2-HIAP-1-Antibody-OTI1A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70184-img0002.jpg)
![Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NBP3-18686-img0001.jpg "Western Blot: PML Protein Antibody [NBP3-18686] - Figure 2. Western blot analysis using the antibody directed against PML Protein Human embryonic kidney cells (293T) were transfected with an ER-tagged PML Protein/RAR fusion protein. Nuclear extracts (50 ug) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the antibody against PML Protein . The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML Protein/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML Protein protein.")
![ELISA: PML Protein Antibody [NBP3-18686] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PML Protein , crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.](http://images.novusbio.com/fullsize/PML Protein Antibody-ELISA-NBP3-18686-img0002.jpg)
![Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222] - Western blot analysis of extracts of various cell lines, using RNF2 Rabbit mAb (NBP3-16222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RNF2-Antibody-9Q2W2-Western-Blot-NBP3-16222-img0001.jpg "Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222] - Western blot analysis of extracts of various cell lines, using RNF2 Rabbit mAb (NBP3-16222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222] - Immunoprecipitation analysis of 300ug extracts of U-937 cells using 3ug RNF2 antibody (NBP3-16222). Western blot was performed from the immunoprecipitate using RNF2 antibody (NBP3-16222) at a dilition of 1:500.](http://images.novusbio.com/fullsize/RNF2-Antibody-9Q2W2-Immunoprecipitation-NBP3-16222-img0002.jpg)
![Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TRIM25-Antibody-4Q0D2-Western-Blot-NBP3-16382-img0001.jpg "Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202511383780.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16382_rabbit-trim25-mab-4q0d2-52202517172871.jpg)
![Western Blot: TRIM21/SSA1 Antibody [NBP3-03809] - Analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21/SSA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/TRIM21-SSA1-Antibody-Knockout-Validated-NBP3-03809-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TRIM21/SSA1 Antibody [NBP3-03809] - Analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21/SSA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP2-97642] - Staining of human RNF113A in human lymph node with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP2-97642-img0003.jpg "Immunohistochemistry-Paraffin: RNF113A Antibody [NBP2-97642] - Staining of human RNF113A in human lymph node with rabbit polyclonal antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: RNF113A Antibody [NBP2-97642] - Staining of human RNF113A in human gallbladder with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF113A-Antibody-Immunohistochemistry-Paraffin-NBP2-97642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RNF113A Antibody [NBP2-97642] - Immunofluorescence staining of RNF113A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97642 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97642_rabbit-polyclonal-rnf113a-antibody-23520251129322.png)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0003.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human lymph node with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP2-98822] - Immunochemical staining of human PML Protein in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-98822-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98822] - Immunofluorescence staining of PML Protein in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98822 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat.](http://images.novusbio.com/fullsize/nbp2-98822_rabbit-polyclonal-pml-protein-antibody-235202521451777.png)
![Western Blot: DTX3L Antibody [NBP3-35870] - Western blot analysis of lysates from Mouse thymus, using DTX3L Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35870_rabbit-dtx3l-pab-912202415121331.jpg "Western Blot: DTX3L Antibody [NBP3-35870] - Western blot analysis of lysates from Mouse thymus, using DTX3L Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: EGR2 Antibody (1F6V7) [NBP3-16157] - Western blot analysis of extracts of various cell lines, using EGR2 Rabbit mAb (NBP3-16157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/EGR2-Antibody-1F6V7-Western-Blot-NBP3-16157-img0001.jpg "Western Blot: EGR2 Antibody (1F6V7) [NBP3-16157] - Western blot analysis of extracts of various cell lines, using EGR2 Rabbit mAb (NBP3-16157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: EGR2 Antibody (1F6V7) [NBP3-16157] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug EGR2 antibody (NBP3-16157). Western blot was performed from the immunoprecipitate using EGR2 antibody (NBP3-16157) at a dilition of 1:500.](http://images.novusbio.com/fullsize/EGR2-Antibody-1F6V7-Immunoprecipitation-NBP3-16157-img0002.jpg)
![Western Blot: TRIM4 Antibody [NBP3-35620] - Western blot analysis of various lysates using TRIM4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35620_rabbit-trim4-pab-912202414213766.jpg "Western Blot: TRIM4 Antibody [NBP3-35620] - Western blot analysis of various lysates using TRIM4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: TRIM4 Antibody [NBP3-35620] - Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35620_rabbit-trim4-pab-3112025170476.jpg%3Ft=1698886109)