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![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-[p-Tyr245]-Antibody-Western-Blot-NBP3-13326-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-[p-Tyr245]-Antibody-Western-Blot-NBP3-13326-img0002.jpg)
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13326_rabbit-c-abl-p-tyr-245-pab-910202420131213.jpg)
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13326_rabbit-c-abl-p-tyr-245-pab-910202420131259.jpg)
![Western Blot: n-Myc Antibody [NBP3-13281] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Western-Blot-NBP3-13281-img0004.jpg "Western Blot: n-Myc Antibody [NBP3-13281] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: n-Myc Antibody [NBP3-13281] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Western-Blot-NBP3-13281-img0001.jpg)
![Western Blot: n-Myc Antibody [NBP3-13281] - IMR32 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Western-Blot-NBP3-13281-img0002.jpg)
![Western Blot: n-Myc Antibody [NBP3-13281] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/n-Myc-Antibody-Western-Blot-NBP3-13281-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13266-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13266-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13266-img0003.jpg)
![Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-Antibody-2C1-RB-Western-Blot-NBP3-13655-img0002.jpg "Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: ATM Antibody (2C1-RB) [NBP3-13655] - ATM antibody [2C1-RB] detects ATM protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.ATM stained by ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13655_rabbit-atm-mab-2c1-rb-91020242063372.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATM Antibody (2C1-RB) [NBP3-13655] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13655_rabbit-atm-mab-2c1-rb-1610202419405781.jpg)
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (NBP3-13655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13655_rabbit-atm-mab-2c1-rb-western-blot-261120251826139.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-159-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-159] - Western blot detection of p14ARF uding NB200-159. Samples: WCE (25 ug) from BT549, positive control and U205 negative control and transfected IMR90 cells.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-159] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-159-img0003.jpg)
![Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody [NB200-159] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-p14ARF used at a dilution of 1:250.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Paraffin-NB200-159-img0001.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-158-img0002.jpg "Western Blot: p14ARF/CDKN2A Antibody [NB200-158] - Samples : Whole cell lysate (25 ug) from BT549 (positive control), U2OS (negative control) and p14ARF/p16INK4a transfected IMR90 cells. Antibody : Affinity purified rabbit anti-p14ARF/p16INK4a used at 1 ug/ml. Detection : Chemiluminescence.")
![Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-158] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p14ARF/p16INK4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-NB200-158-img0003.jpg)
![Western Blot: Menin Antibody [NB100-287] - Using the Biotin direct conjugate Samples: Whole cell lysate from HEK 293T (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, goat anti-Menin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0092.jpg "Western Blot: Menin Antibody [NB100-287] - Using the Biotin direct conjugate Samples: Whole cell lysate from HEK 293T (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, goat anti-Menin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Menin Antibody [NB100-287] - Samples: Whole cell lysate (40 ug for WB; one 10 cm plate 293T cells for IP) from 293T cells. Affinity purified goat anti-Menin used as indicated for IP. Menin was detected by Western Blot using affinity purified rabbit anti-Menin NB100-215 at 0.1 ug/ml for WB. Detection: Chemiluminescence exposed for 1 minute.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0003.jpg)
![Western Blot: Menin Antibody [NB100-287] - Analysis using the Biotin conjugate of NB100-287. Detection of HEK 293T (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0090.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Menin Antibody [NB100-287] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Menin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Menin-Antibody-Western-Blot-NB100-287-img0091.jpg)
![Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP1-19102-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-19102-img0003.jpg)
![Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32121-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0004.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0003.jpg)
![Western Blot: NORE1 Antibody (1H2) [NBP2-03691] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NORE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NORE1.](http://images.novusbio.com/fullsize/NORE1-Antibody-1H2-Western-Blot-NBP2-03691-img0004.jpg "Western Blot: NORE1 Antibody (1H2) [NBP2-03691] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NORE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NORE1.")
![Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NORE1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NORE1-Antibody-1H2-Flow-Cytometry-NBP2-03691-img0003.jpg)
![Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - Analysis of Hela cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NORE1-Antibody-1H2-Flow-Cytometry-NBP2-03691-img0001.jpg)
![Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - Analysis of Jurkat cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NORE1-Antibody-1H2-Flow-Cytometry-NBP2-03691-img0002.jpg)
![In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-In-vitro-assay-NBP3-14783-img0001.jpg "In vitro assay: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - Thioflavin T assay of SOD1 pre-formed fibrils (PFFs) (NBP3-14783) combined with SOD1 monomers . Pre-formed fibrils seed the aggregation of monomers, leading to an increase in Thioflavin T fluorescence")
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant Human SOD1-Cu-Zn SOD Pre-formed Fibrils Protein-Electron Microscopy-NBP3-14783-img0003.jpg)
![Electron Microscopy: Recombinant Human SOD1/Cu-Zn SOD Pre-formed Fibrils Protein [NBP3-14783] - TEM of human SOD1 pre-formed fibrils (PFFs)](http://images.novusbio.com/fullsize/Recombinant-Human-SOD1-Cu-Zn-SOD-Pre-formed-Fibrils-Protein-Electron-Microscopy-NBP3-14783-img0002.jpg)
![ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Samples were spiked with high concentrations of Mouse Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69946_mouse-bcl-2-elisa-kit-colorimetric-132202416221063.jpg "ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Samples were spiked with high concentrations of Mouse Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69946_mouse-bcl-2-elisa-kit-colorimetric-4320248582563.jpg)