| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94894-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35658-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03252-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52578-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52549-0.025mg | |||
![Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Western-Blot-NBP3-16268-img0006.jpg "Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Mouse spinal cord using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Rat brain using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of NIH/3T3 cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-5220251265370.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of U-2 OS cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-522025125414.jpg)
![Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of various cell lines, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/RbAp46-Antibody-Western-Blot-NBP2-94894-img0002.jpg "Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of various cell lines, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Chromatin Immunoprecipitation: RbAp46 Antibody [NBP2-94894] - Analysis of extracts of 293T cells, using RbAp46 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/RbAp46-Antibody-Chromatin-Immunoprecipitation-NBP2-94894-img0001.jpg)
![Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of Rat spleen, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RbAp46 Antibody-Western Blot-NBP2-94894-img0003.jpg)
![Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202414163078.jpg "Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415143678.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP3-35658] - Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-91220241519588.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415121317.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-9122024151959.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Knockout-Validated-NBP3-03252-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-5220251628098.jpg)
![Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of C6 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-52202516293915.jpg)
![Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of L-929 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-52202516345261.jpg)
![Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Western-Blot-NBP2-52578-img0002.jpg "Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)")
![Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded colon cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Immunohistochemistry-Paraffin-NBP2-52578-img0006.jpg)
![Flow Cytometry: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of Hela cells using TUBE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Flow-Cytometry-NBP2-52578-img0001.jpg)
![Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - 2: Western blot analysis using TUBE1 mAb against HEK293 (1) and TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Western-Blot-NBP2-52578-img0003.jpg)
![Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded liver cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Immunohistochemistry-Paraffin-NBP2-52578-img0007.jpg)
![ELISA: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-ELISA-NBP2-52578-img0004.jpg)
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0004.jpg "Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)")
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded esophageal cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0003.jpg)
![Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Flow-Cytometry-NBP2-52549-img0001.jpg)
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against HEK293 (1) and RAD23A (AA: 1-363)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded bladder cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0002.jpg)
![ELISA: Rad23 Antibody (3E3F4) [NBP2-52549] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Rad23-Antibody-3E3F4-ELISA-NBP2-52549-img0006.jpg)
![Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Western-Blot-NBP2-73783-img0002.jpg "Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.")
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0006.jpg)
![Western Blot: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Western-Blot-NBP2-73783-img0001.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0003.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0004.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0005.jpg)
![Immunohistochemistry: Rad9 Antibody (OTI7B11) - Azide and BSA Free [NBP2-73783] - Staining of paraffin-embedded colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-OTI7B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73783-img0007.jpg)
![Western Blot: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Western-Blot-NBP2-72311-img0001.jpg "Western Blot: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF .")
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0005.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min).](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0002.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0003.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0004.jpg)
![Immunohistochemistry: Bmf Antibody (OTI6F3) - Azide and BSA Free [NBP2-72311] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bmf-Antibody-OTI6F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72311-img0006.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0002.jpg "Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.")
![Immunocytochemistry/Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70739-img0005.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0004.jpg)
![Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Western-Blot-NBP2-70739-img0001.jpg)
![Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/FHL1-Antibody-OTI3G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70739-img0003.jpg)
![Western Blot: Bag-1 Antibody (JE63-22) [NBP3-32068] - Western blot analysis of Bag-1 on different lysates with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 35/46/52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-27820241631691.jpg "Western Blot: Bag-1 Antibody (JE63-22) [NBP3-32068] - Western blot analysis of Bag-1 on different lysates with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 35/46/52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bag-1 Antibody (JE63-22) [NBP3-32068] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32068) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-298202414304135.jpg)
![Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (JE63-22) [NBP3-32068] - Immunocytochemistry analysis of HeLa cells labeling Bag-1 with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-29202410334084.jpg)
![Flow Cytometry: Bag-1 Antibody (JE63-22) [NBP3-32068] - Flow cytometric analysis of HeLa cells labeling Bag-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32068, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-29202416372155.jpg)
![Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-288202412442995.jpg "Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)