| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86860-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-724-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43313-0.5mg | |||
| BSA Free | NBP1-43313-0.05mg | |||
| BSA Free | NBP1-43313-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35533-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74026-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94894-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35658-20ul | |||
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-212024941427.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-261120251839316.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunohistochemistry-paraffin-26112025195717.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-26112025199124.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518224316.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518463713.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-261120251818503.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0011.jpg "Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0008.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0009.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscles and glandular cells.](http://images.novusbio.com/fullsize/LATS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0010.jpg)
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0003.jpg "Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.")
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR (NB 600-724) visualised with F(ab')2 RABBIT ANTI RAT IgG: FITC secondary.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0001.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0005.jpg "Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.")
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - An intracellular stain was performed on RAW 246.7 cells with Galectin-3 Antibody (M3/38) NBP1-43313AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0003.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0004.jpg)
![Western Blot: p73 Antibody (ER-15) [NB600-1279] - Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using p73 alpha/beta Monoclonal Antibody, shows increased levels of p73 alpha/beta in MCF7 cell line upon doxorubicin treatment.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Western-Blot-NB600-1279-img0003.jpg "Western Blot: p73 Antibody (ER-15) [NB600-1279] - Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using p73 alpha/beta Monoclonal Antibody, shows increased levels of p73 alpha/beta in MCF7 cell line upon doxorubicin treatment.")
![Western Blot: p73 Antibody (ER-15) [NB600-1279] - WB analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1) and MCF7 treated with Doxorubicine (0.5 uM Doxorubicin for 24 hours) (Lane 2). The blot was probed with Anti-p73 alpha/beta Mouse Monoclonal Antibody.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Western-Blot-NB600-1279-img0001.jpg)
![Immunofluorescence: p73 Antibody (ER-15) [NB600-1279] - Analysis of p73 alpha/beta was performed using 70% confluent log phase MCF-7 cells.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Immunofluorescence-NB600-1279-img0002.jpg)
![Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Western-Blot-NBL1-13748-img0003.jpg "Western Blot: Nucleophosmin Overexpression Lysate [NBL1-13748] - Analysis of Nucleophosmin Overexpression Lysate using Nucleophosmin antibody as a positive control. Image provided via product review by verified customer.")
![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](http://images.novusbio.com/fullsize/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg)
![Western Blot: ASC1 Antibody [NBP3-35533] - Western blot analysis of various lysates using ASC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-101220241139294.jpg "Western Blot: ASC1 Antibody [NBP3-35533] - Western blot analysis of various lysates using ASC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunoprecipitation: ASC1 Antibody [NBP3-35533] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug ASC1 antibody. Western blot was performed from the immunoprecipitate using ASC1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411392973.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Rat heart using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411343334.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-101220241143340.jpg)
![Immunohistochemistry: ASC1 Antibody [NBP3-35533] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using ASC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35533_rabbit-asc1-pab-1012202411421060.jpg)
![Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Western-Blot-NBP1-74026-img0009.jpg "Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Immunocytochemistry-Immunofluorescence-NBP1-74026-img0006.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0011.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0007.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0010.jpg)
![Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of various cell lines, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/RbAp46-Antibody-Western-Blot-NBP2-94894-img0002.jpg "Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of various cell lines, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Chromatin Immunoprecipitation: RbAp46 Antibody [NBP2-94894] - Analysis of extracts of 293T cells, using RbAp46 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/RbAp46-Antibody-Chromatin-Immunoprecipitation-NBP2-94894-img0001.jpg)
![Western Blot: RbAp46 Antibody [NBP2-94894] - Western blot analysis of extracts of Rat spleen, using RbAp46 antibody (NBP2-94894) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RbAp46 Antibody-Western Blot-NBP2-94894-img0003.jpg)
![Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202414163078.jpg "Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415143678.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP3-35658] - Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-91220241519588.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415121317.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-9122024151959.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/WT1-Antibody-6L9X6-Western-Blot-NBP3-16135-img0006.jpg "Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-5220251242594.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-522025125482.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111235..jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human kidney tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111344..jpg)
![Immunohistochemistry: WT1 Antibody (6L9X6) [NBP3-16135] - Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-52202511361241.jpg)
![Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Western-Blot-NBP3-16268-img0006.jpg "Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Mouse spinal cord using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Rat brain using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of NIH/3T3 cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-5220251265370.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Confocal imaging of U-2 OS cells using PUMA Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16268_rabbit-puma-mab-9c5w5-522025125414.jpg)