| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02974-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61715-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33313 | |||
| IHC-Prediluted | NBP2-44779 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-599SS | |||
| Azide and BSA Free | NBP2-80601 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83249-25ul | |||
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP3-02974-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ERK2 Antibody [NBP3-02974] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02974-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0003.jpg)
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts of various cell lines, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2 Antibody-Western Blot-NBP3-02974-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of lysates from wild type (WT) and ERK2 knockout (KO) HeLa cells using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. <br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit <br>Exposure time: 90 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251636384.jpg)
![Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of various lysates using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. <br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit <br>Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251632146.jpg)
![Western Blot: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Western blot analysis of lysates from Jurkat cells, using DNA Ligase I Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-101220241252825.jpg "Western Blot: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Western blot analysis of lysates from Jurkat cells, using DNA Ligase I Rabbit mAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.")
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human colon carcinoma tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-3112025173151.jpg%3Ft=1705995101)
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded rat lung tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-31120251744428.jpg%3Ft=1705995101)
![Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunofluorescence analysis of C6 cells using DNA Ligase I Rabbit mAb (A9301) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-1012202411531050.jpg)
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded rat brain tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-31120251744482.jpg%3Ft=1705995101)
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human thyroid cancer tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-3112025174448.jpg%3Ft=1705995101)
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human spleen tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-3112025174446.jpg%3Ft=1705995101)
![Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded mouse brain tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33540_rabbit-dna-ligase-i-mab-0d6m4-3112025177015.jpg%3Ft=1705995101)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0006.jpg "Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunocytochemistry-Immunofluorescence-NBP2-61715-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of rectum cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0005.jpg)
![Flow Cytometry: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Flow-Cytometry-NBP2-61715-img0002.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against HEK293 (1) and BNIP3 (AA: 50-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of breast cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0004.jpg)
![ELISA: BNIP3 Antibody (6A5F7) [NBP2-61715] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-ELISA-NBP2-61715-img0001.jpg)
0.05, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001 and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001, and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to Control; +p < 0.05, ++p < 0.01, + + +p < 0.001, and + + + +p < 0.0001 compared to Cocl2; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 compared to Hypoxia; ≠p < 0.05, ≠≠p < 0.01, ≠≠≠p < 0.001, and ≠≠≠≠p < 0.0001 compared to si-becn1 (21%O2) Vs, si-becn1 (1%O2). In western blots “→" represents a specific signal. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35928940), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" />
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human spleen shows moderate to strong cytoplasmic positivity.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0019.jpg "Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human spleen shows moderate to strong cytoplasmic positivity.")
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0015.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0016.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0018.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16841-img0009.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded mouse brain. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16841-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HIST1H3A Antibody [NBP2-16841] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16841_rabbit-polyclonal-hist1h3a-antibody-1610202419414554.jpg)
![Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Free version of NBP2-29630. Detection of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Western-Blot-NBP2-29630-img0003.jpg "Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Free version of NBP2-29630. Detection of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Immunohistochemistry-Paraffin-NBP2-29630-img0005.jpg)
![Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis of Bcl-2 in human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Western-Blot-NBP2-29630-img0001.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0008.jpg "Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (KEN) [NB100-599] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BRCA1 (KEN) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Immunocytochemistry-Immunofluorescence-NB100-599-img0009.jpg)
![Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1.](http://images.novusbio.com/fullsize/BRCA1-Antibody-KEN-Western-Blot-NB100-599-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Immunocytochemistry-Immunofluorescence-DDX0140P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - FACS staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Flow-Cytometry-DDX0140P-100-img0002.jpg)
![Neutralization: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - Inhibition of IL12 production by CD40L-stimulated human monocyte-derived DCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Neutralization-DDX0140P-100-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0009.jpg "Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00004001-M01-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0006.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0007.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0008.jpg)
![Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunoprecipitation-H00004001-M01-img0004.jpg)
![ELISA: Lamin B1 Antibody (4B10) [H00004001-M01] - Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-ELISA-H00004001-M01-img0010.jpg)
![Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Western-Blot-H00000891-M01-img0007.jpg "Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to Cyclin B1 on formalin-fixed paraffin-embedded human tonsil. Antigen retrieval was done enzymatically. Primary antibody dilution was 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to CCNB1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0005.jpg)
![ELISA: Cyclin B1 Antibody (1C8) [H00000891-M01] - Detection limit for recombinant GST tagged CCNB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-ELISA-H00000891-M01-img0008.jpg)
![Western Blot: DDX6 Antibody [NBP1-83249] - Analysis using Anti-DDX6 antibody NBP1-83249 (A) shows similar pattern to independent antibody NBP1-83248 (B).](http://images.novusbio.com/fullsize/DDX6-Antibody-Western-Blot-NBP1-83249-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX6 Antibody [NBP1-83249] - Analysis using Anti-DDX6 antibody NBP1-83249 (A) shows similar pattern to independent antibody NBP1-83248 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NBP1-83249] - Staining of human cell line A-431 shows localization to cytoplasmic bodies.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83249-img0007.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0013.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0011.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0012.jpg)
![Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/DDX6-Antibody-Immunohistochemistry-Paraffin-NBP1-83249-img0014.jpg)
