| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| BSA Free | NBP3-07012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56141SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47910-0.1mg | |||
| IHC-Prediluted | NBP2-48332 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Western-Blot-NBP1-32213-img0030.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p27/Kip1 Antibody [NBP1-32213] - Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NBP1-32213] - Staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunohistochemistry-Frozen-NBP1-32213-img0011.jpg)
![Immunoprecipitation: p27/Kip1 Antibody [NBP1-32213] - Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 ug of p27 Kip1 antibody. Western blot analysis was performed using p27 Kip1 antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-Immunoprecipitation-NBP1-32213-img0023.jpg)
![Western Blot: p27/Kip1 Antibody [NBP1-32213] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (NBP1-32213) diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-32213_rabbit-polyclonal-p27-kip1-antibody-western-blot-512024112649..jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg "Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014664.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115087.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242011459.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-9102024205654.jpg)
![Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-immunocytochemistry-immunofluorescence-261120251823621.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33177-img0008.jpg)
![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - H1299 xenograft, using Gelsolin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunohistochemistry-Paraffin-NBP1-33177-img0009.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0011.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0012.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33177-img0014.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (NBP1-33177) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-33177_rabbit-polyclonal-gelsolin-gsn-antibody-western-blot-2611202518233017.jpg)
![Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (NBP1-33177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33177_rabbit-polyclonal-gelsolin-gsn-antibody-western-blot-2611202518315411.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0015.jpg "Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20534-img0014.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0002.jpg)
![Western Blot: Superoxide Dismutase 1 Antibody [NBP2-20534] - Sample (50 ug of whole cell lysate) A: Rat brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Superoxide-Dismutase-1-Antibody-Western-Blot-NBP2-20534-img0003.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-20534-img0013.jpg)
![Immunohistochemistry-Frozen: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain. Green: SOD1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: beta Tubulin 3/ TUJ1 antibody diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Frozen-NBP2-20534-img0010.jpg)
![ELISA: SOD1/Cu-Zn SOD Antibody [NBP2-20534] - ELISA detection of SOD1 using SOD1 Antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/SOD1-Cu-Zn-SOD-Antibody-ELISA-NBP2-20534-img0012.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0019.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:600 and with DDDDK tag antibody (NBP2-43574) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0007.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NBP2-15791-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15791-img0022.jpg)
![Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0021.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0020.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-91020242075966.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-1610202419405783.jpg)
![Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265] - Detection of Human and Mouse UBCH7 one HeLa whole cell lysate using NB100-2265.](http://images.novusbio.com/fullsize/UbcH7-UBE2L3-Antibody-Western-Blot-NB100-2265-img0011.jpg "Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265] - Detection of Human and Mouse UBCH7 one HeLa whole cell lysate using NB100-2265.")
![Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti- UBCH7 antibody used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.](http://images.novusbio.com/fullsize/UbcH7-UBE2L3-Antibody-Immunohistochemistry-NB100-2265-img0012.jpg)
![Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - Human breast tumors stained with UbcH7 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/UbcH7-UBE2L3-Antibody-Immunohistochemistry-Paraffin-NB100-2265-img0004.jpg)
![Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Western-Blot-NB200-184-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CDC73-HRPT2-Antibody-Immunoprecipitation-NB200-184-img0007.jpg)
![Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Western-Blot-NBP2-33250-img0004.jpg "Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33250-img0002.jpg)
![Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33250-img0003.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0007.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0006.jpg)
![Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/AIF-Antibody-Knockout-Validated-NBP2-27177-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and AIF knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human AIF Polyclonal Antibody (Catalog # NBP2-27177) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 68 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: AIF Antibody [NBP2-27177] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF.](http://images.novusbio.com/fullsize/AIF-Antibody-Western-Blot-NBP2-27177-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-27177] - Staining of AIF in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. Hematoxylin-eosin counterstain. In this example, AIF expression is predominant in the upper part of the crypt.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-27177-img0002.jpg)
![Simple Western: AIF Antibody [NBP2-27177] - Simple Western lane view shows a specific band for AIF in 0.2 mg/ml of Jurkat and MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Simple-Western-NBP2-27177-img0003.jpg)
![Western Blot: FLIP Antibody [NB100-56141] - analysis of FLIP in human ovarian cancer cell lines using anti-FLIP antibody. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C in 5% milk in PBST. Image from verified customer review.](http://images.novusbio.com/fullsize/FLIP-Antibody-Western-Blot-NB100-56141-img0003.jpg "Western Blot: FLIP Antibody [NB100-56141] - analysis of FLIP in human ovarian cancer cell lines using anti-FLIP antibody. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C in 5% milk in PBST. Image from verified customer review.")
![Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/FLIP-Antibody-Immunohistochemistry-NB100-56141-img0002.jpg)
![Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/FLIP-Antibody-Immunohistochemistry-NB100-56141-img0001.jpg)
![Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-NBP2-44568-img0005.jpg "Immunohistochemistry: CD34 Antibody (HPCA1/763) [NBP2-44568] - Characterization of human SVF cells on aligned nanofibrillar collagen scaffolds. Confocal image shows CD34 protein expression among adherent SVF cells on aligned nanofibrillar scaffolds after 1 day of attachment. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Placenta stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunofluorescence-NBP2-44568-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - Immunofluorescence staining of retained human SVF adjacent to the site of cell-seeded scaffold transplantation. (A) Human SVF are visualized using human specific nuclear matrix antigen (NuMA). Immunofluorescence imaging of human-specific antibodies targeting CD34 (B) & CD31 (C). Scale bar: 50 μm (A), 100 μm (B,C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44568_mouse-monoclonal-cd34-antibody-hpca1-763-31020241535640.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)