| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04468-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26297SS | |||
| Azide and BSA Free | NBP2-80867 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44978 | |||
| Azide and BSA Free | NBP2-34605-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50115-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87780-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08957 | |||
![Western Blot: ELF5 Antibody [NBP3-04468] - Analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ELF5-Antibody-Western-Blot-NBP3-04468-img0002.jpg "Western Blot: ELF5 Antibody [NBP3-04468] - Analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: ELF5 Antibody [NBP3-04468] - Analysis of A549 cells using ELF5 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04468-img0001.jpg)
![Immunohistochemistry-Paraffin: ELF5 Antibody [NBP3-04468] - Rat liver using ELF5 Rabbit pAb at dilution of 1:50 (40x lens).](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunohistochemistry-Paraffin-NBP3-04468-img0004.jpg)
![Immunohistochemistry-Paraffin: ELF5 Antibody [NBP3-04468] - Mouse brain using ELF5 Rabbit pAb at dilution of 1:50 (40x lens).](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunohistochemistry-Paraffin-NBP3-04468-img0003.jpg)
![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Knockout-Validated-NBP2-26297-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunocytochemistry-Immunofluorescence-NBP2-26297-img0008.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0011.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red).](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Flow-Cytometry-NBP2-26297-img0009.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0010.jpg)
![ELISA: Nbs1 Antibody (7E4A2) [NBP2-26297] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297].](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-ELISA-NBP2-26297-img0014.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Simple-Western-NBP2-26297-img0017.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.<br></br>Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: p53 protein stained by p53 antibody [N1], N-term.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.<br></br>Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: p53 protein stained by p53 antibody [N1], N-term.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Western-Blot-NBP2-34308-img0004.jpg "Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).")
![Immunohistochemistry-Paraffin: p53 Antibody (DO-7) [NBP2-34308] - Formalin-fixed, paraffin-embedded human colon stained with p53 MAb (DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Immunohistochemistry-Paraffin-NBP2-34308-img0001.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0002.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0003.jpg)
![Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Western-Blot-NBP2-80102-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Bim Antibody (3C5) [NBP2-80102] - Detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: Bim Antibody (3C5) [NBP2-80102] - Data in permeabilized and fixed transfected cells using anti-Bim, mAb (3C5) anti-EE (positive control) or secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.](http://images.novusbio.com/fullsize/Bim-Antibody-3C5-Flow-Cytometry-NBP2-80102-img0001.jpg)
![Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Western-Blot-NBP2-49695-img0004.jpg "Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.")
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695] - taining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Immunocytochemistry-Immunofluorescence-NBP2-49695-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Immunohistochemistry-Paraffin-NBP2-49695-img0001.jpg)
![Western Blot: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of various lysates of transfectants expressing different BCL11A isoforms and the highly homologous BCL11B to show specificity for BCL11AXL.](http://images.novusbio.com/fullsize/Ctip1-Antibody-BCL11A-123-Western-Blot-NBP2-50115-img0004.jpg "Western Blot: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of various lysates of transfectants expressing different BCL11A isoforms and the highly homologous BCL11B to show specificity for BCL11AXL.")
![Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Analysis of Ctip1 expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones. Double labelling studies show the strongly labelled cells in the interfollicular areas to be CD123-positive pDCs (arrowhead).](http://images.novusbio.com/fullsize/Ctip1-Antibody-BCL11A-123-Immunocytochemistry-Immunofluorescence-NBP2-50115-img0005.jpg)
![Immunohistochemistry: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL (BCL123a) expression in tonsil BCL11AXL is present in B cells (brown) in germinal centres and mantle zones.](http://images.novusbio.com/fullsize/Ctip1-Antibody-BCL11A-123-Immunohistochemistry-NBP2-50115-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - BCL11AXL is absent in plasma cells BCL11A+ cells (blue) /CD3+ T cells (red) / p63-positive plasma cells (green)](http://images.novusbio.com/fullsize/Ctip1-Antibody-BCL11A-123-Immunocytochemistry-Immunofluorescence-NBP2-50115-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ctip1 Antibody (BCL11A/123) [NBP2-50115] - Nuclear staining of COS/ BCL11AXL transfectants but not BCL BCL11AL transfectants using antibody BCL123a.](http://images.novusbio.com/fullsize/Ctip1-Antibody-BCL11A-123-Immunocytochemistry-Immunofluorescence-NBP2-50115-img0003.jpg)
![Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Western-Blot-NB100-56150-img0001.jpg "Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NB100-56150-img0002.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87780) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Knockdown-Validated-NBP1-87780-img0024.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87780) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in human skin and skeletal muscle tissues. Corresponding SERPINB5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex, liver, skin and testis using Anti-SERPINB5 antibody NBP1-87780 (A) shows similar protein distribution across tissues to independent antibody NBP1-87779 (B).](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0022.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in human cell lines PC-3 and Caco-2 using anti-SERPINB5 antibody. Corresponding SERPINB5 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87780-img0006.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0020.jpg)
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0011.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human testis.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0014.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human liver.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0015.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0016.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0017.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skin shows strong positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0018.jpg)
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human upper gastrointestinal shows weak to moderate cytoplasmic/membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0019.jpg)
![Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Western-Blot-NBP3-07211-img0007.jpg "Western Blot: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).")
![Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunocytochemistry-Immunofluorescence-NBP3-07211-img0002.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0006.jpg)
![Flow Cytometry: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Flow-Cytometry-NBP3-07211-img0001.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0003.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0004.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) [NBP3-07211] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).](http://images.novusbio.com/fullsize/MSH2-Antibody-MSH2-2622-Immunohistochemistry-Paraffin-NBP3-07211-img0005.jpg)