| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99086-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97161-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21561-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21566-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21563-50ul | |||
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235--p-Ser236]-Antibody-Western-Blot-NB100-1553-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).")
![Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NBP2-87261-img0001.jpg "Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells")
![Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05462_rabbit-polyclonal-ampk-alpha-1-p-ser485-antibody-52202516334154.jpg "Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Western-Blot-NBP2-99086-img0002.jpg "Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086] - Thymidylate Synthase was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate4 ul anti-Thymidylate Synthase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Thymidylate Synthase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Immunoprecipitation-NBP2-99086-img0001.jpg)
![Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-97161] - Staining of human PUM2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunohistochemistry-Paraffin-NBP2-97161-img0002.jpg "Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-97161] - Staining of human PUM2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.")
![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-97161] - Immunofluorescence staining of PUM2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97161 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97161_rabbit-polyclonal-pum2-antibody-235202511254410.png)
![Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] - Western blot analysis using NBP3-21561 in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21561_rabbit-ampk-alpha-1-p-ser-496-mab-sr2088-5720231456543.jpg "Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] - Western blot analysis using NBP3-21561 in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.")
![Western Blot: AMPK alpha 2 [p Ser 491] Antibody (SR2078) [NBP3-21566] - Western blot analysis using NBP3-21566 in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21566_rabbit-ampk-alpha-2-p-ser-491-mab-sr2078-5720231624735.jpg "Western Blot: AMPK alpha 2 [p Ser 491] Antibody (SR2078) [NBP3-21566] - Western blot analysis using NBP3-21566 in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.")
![Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] - Western blot analysis using NBP3-21563 in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21563_rabbit-ampk-alpha-2-p-ser-345-mab-sr2089-5720231624719.jpg "Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] - Western blot analysis using NBP3-21563 in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.")
![Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/RPS6-[p-Ser244]-Antibody-Western-Blot-NBP2-29653-img0001.jpg "Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).")
![SDS-Page: Recombinant Human AMPK alpha 2 Protein [H00005563-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005563-Q01-1.jpg "SDS-Page: Recombinant Human AMPK alpha 2 Protein [H00005563-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human DDX17 Protein [H00010521-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010521-P01-1.jpg "SDS-Page: Recombinant Human DDX17 Protein [H00010521-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - PRKAA1 monoclonal antibody (M01), clone 1G4 Analysis of PRKAA1 expression in HepG2.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-1G4-Western-Blot-H00005562-M01-img0004.jpg "Western Blot: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - PRKAA1 monoclonal antibody (M01), clone 1G4 Analysis of PRKAA1 expression in HepG2.")
![ELISA: AMPK alpha 1 Antibody (1G4) [H00005562-M01] - Detection limit for recombinant GST tagged PRKAA1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-1G4-ELISA-H00005562-M01-img0003.jpg)