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![Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Western-Blot-NBP3-19825-img0002.jpg "Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Immunocytochemistry of AMPK alpha 1 (phospho-Ser496) (green) in hela using NBP3-19825 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Immunocytochemistry-Immunofluorescence-NBP3-19825-img0001.jpg)
![Recombinant Human TOR/mTOR Protein [H00002475-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002475-Q01-1.jpg "Recombinant Human TOR/mTOR Protein [H00002475-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (rTYMS/1884) [NBP3-07145] - Immunofluorescence Analysis of PFA fixed MOLT-4 cells labeling Thymidylate Synthase Recombinant Mouse Monoclonal (rTYMS/1884) followed by Goat anti- Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-rTYMS-1884-Immunocytochemistry-Immunofluorescence-NBP3-07145-img0002.jpg "Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (rTYMS/1884) [NBP3-07145] - Immunofluorescence Analysis of PFA fixed MOLT-4 cells labeling Thymidylate Synthase Recombinant Mouse Monoclonal (rTYMS/1884) followed by Goat anti- Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).")
![Flow Cytometry: Thymidylate Synthase Antibody (rTYMS/1884) [NBP3-07145] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. Thymidylate Synthase Recombinant Mouse Monoclonal Antibody (rThymidylate Synthase/1884) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-rTYMS-1884-Flow-Cytometry-NBP3-07145-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-Antibody-GT829-Western-Blot-NBP3-13571-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-Antibody-GT829-Western-Blot-NBP3-13571-img0001.jpg)
![Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-07452] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Immunohistochemistry-Paraffin-NBP3-07452-img0002.jpg "Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-07452] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)")
![Protein Array: YB1 Antibody (YBX1/2430) [NBP3-07452] - Analysis of Protein Array containing more than 19,000 full-length human proteins using YB1 Mouse Monoclonal Antibody (YB1/2430).](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Protein-Array-NBP3-07452-img0001.jpg)
![Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05462_rabbit-polyclonal-ampk-alpha-1-p-ser485-antibody-52202516334154.jpg "Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-288202412461618.jpg "Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-392024884690.jpg)
![Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Western-Blot-NBP2-99086-img0002.jpg "Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086] - Thymidylate Synthase was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate4 ul anti-Thymidylate Synthase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Thymidylate Synthase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-Immunoprecipitation-NBP2-99086-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32919) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: HCT 116 cell lysate Lane 6: THP-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32919) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32919_mouse-ribosomal-protein-s6-rps6-mab-a6b8-r-288202412461617.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32919) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: HCT 116 cell lysate Lane 6: THP-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32919) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NBP2-87261-img0001.jpg "Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells")