| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61816-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61686-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37501-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-223SS | |||
![Western Blot: Olig2 Antibody (1G11) [NBP2-37636] - Western blot analysis using OLIG2 mAb against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Western-Blot-NBP2-37636-img0002.jpg "Western Blot: Olig2 Antibody (1G11) [NBP2-37636] - Western blot analysis using OLIG2 mAb against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Olig2 Antibody (1G11) [NBP2-37636] - Immunofluorescence analysis of U251 cells using OLIG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Immunocytochemistry-Immunofluorescence-NBP2-37636-img0003.jpg)
![Immunohistochemistry: Olig2 Antibody (1G11) [NBP2-37636] - Immunohistochemical analysis of paraffin-embedded muscle tissues using OLIG2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-Immunohistochemistry-NBP2-37636-img0004.jpg)
![ELISA: Olig2 Antibody (1G11) [NBP2-37636] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Olig2-Antibody-1G11-ELISA-NBP2-37636-img0001.jpg)
![Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)](http://images.novusbio.com/fullsize/PXR-NR1I2-Antibody-1D12G1-Western-Blot-NBP2-61816-img0004.jpg "Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)")
![Immunocytochemistry/Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of Hela cells using NR1I2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 Secondary antibody was used.](http://images.novusbio.com/fullsize/PXR-NR1I2-Antibody-1D12G1-Immunocytochemistry-Immunofluorescence-NBP2-61816-img0003.jpg)
![Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against HEK293 (1) and NR1I2 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PXR-NR1I2-Antibody-1D12G1-Western-Blot-NBP2-61816-img0005.jpg)
![ELISA: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/PXR-NR1I2-Antibody-1D12G1-ELISA-NBP2-61816-img0001.jpg)
![ELISA: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of HepG2 cells using NR1I2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PXR-NR1I2-Antibody-1D12G1-ELISA-NBP2-61816-img0002.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0004.jpg "Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)")
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0003.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0005.jpg)
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded esophageal cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0002.jpg)
![ELISA: ASH2L Antibody (6B10H10) [NBP2-61686] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-ELISA-NBP2-61686-img0001.jpg)
![Western Blot: CEBP alpha Antibody (4E10) [NBP2-37501] - Western blot analysis using CEBPA mouse mAb against THP-1 (1)cell lysate.](http://images.novusbio.com/fullsize/CEBP-alpha-Antibody-4E10-Western-Blot-NBP2-37501-img0003.jpg "Western Blot: CEBP alpha Antibody (4E10) [NBP2-37501] - Western blot analysis using CEBPA mouse mAb against THP-1 (1)cell lysate.")
![Flow Cytometry: CEBP alpha Antibody (4E10) [NBP2-37501] - An intracellular stain was performed on HepG2 cells with NBP2-37501APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/CEBP-alpha-Antibody-4E10-NBP2-37501-img0005.jpg)
![Western Blot: CEBP alpha Antibody (4E10) [NBP2-37501] - Western blot analysis using CEBPA mAb against human CEBPA (AA: 139-204) recombinant protein. (Expected MW is 32.7 kDa)](http://images.novusbio.com/fullsize/CEBP-alpha-Antibody-4E10-Western-Blot-NBP2-37501-img0002.jpg)
![Flow Cytometry: CEBP alpha Antibody (4E10) [NBP2-37501] - Flow cytometric analysis of HeLa cells using CEBPA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CEBP-alpha-Antibody-4E10-Flow-Cytometry-NBP2-37501-img0004.jpg)
![ELISA: CEBP alpha Antibody (4E10) [NBP2-37501] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CEBP-alpha-Antibody-4E10-ELISA-NBP2-37501-img0001.jpg)
![Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Western-Blot-NB600-223-img0001.jpg "Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.")
![Simple Western: HDAC10 Antibody [NB600-223] - 1:25 dilution against the indicated concentration of protein lysate (Human SVG-A). Image from verified customer review.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Simple-Western-NB600-223-img0002.jpg)
![Western Blot: Zic2 Antibody [NBP2-86425] - Host: Rabbit. Target Name: ZIC2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Zic2-Antibody-Western-Blot-NBP2-86425-img0004.jpg "Western Blot: Zic2 Antibody [NBP2-86425] - Host: Rabbit. Target Name: ZIC2. Sample Tissue: Human HT1080 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: Zic2 Antibody [NBP2-86425] - Human Skin](http://images.novusbio.com/fullsize/Zic2-Antibody-Immunohistochemistry-NBP2-86425-img0001.jpg)
![Western Blot: Zic2 Antibody [NBP2-86425] - WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/Zic2-Antibody-Western-Blot-NBP2-86425-img0003.jpg)
![Immunoprecipitation: Zic2 Antibody [NBP2-86425] - ZIC2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with NBP2-86425 with 1:200 dilution. Western blot was performed using NBP2-86425 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: ZIC2 IP with NBP2-86425](http://images.novusbio.com/fullsize/Zic2-Antibody-Immunoprecipitation-NBP2-86425-img0005.jpg)
![Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-28820241423310.jpg "Western Blot: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: U87-MG cell lysate Lane 6: MG-63 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33133) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg)
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-288202412493482.jpg "Western Blot: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-298202414304168.jpg)
![Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Immunocytochemistry analysis of HepG2 cells labeling AP2 alpha with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-29202410234285.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of non-small cell carcinoma of the lung.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-NBP3-14518-img0005.jpg "Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of non-small cell carcinoma of the lung.")
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0001.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0002.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, non-small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0003.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of brain, glioblastoma.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-NBP3-14518-img0004.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of ovary carcinoma](http://images.novusbio.com/fullsize/WNK1 Antibody-Immunohistochemistry-NBP3-14518-img0006.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)