TetR Antibody [DyLight 405]

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Product Details

Summary
Reactivity NASpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

TetR Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to an N-terminal portion of Escherichia coli TetR protein (between residues 1-50) [UniProt P04483]
Localization
TetR peptide conserved in all strains of gram negative bacteria
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This TetR antibody is useful for Western blot.

Reactivity Notes

This antibody is used to monitor the regulation of gene expression in bacteria cells.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TetR Antibody [DyLight 405]

  • pSC101 tetR
  • Tet Repressor Protein
  • Tet Repressor
  • TetR
  • tetracycline repressor protein

Background

Tetracycline repressor TetR (tetracycline repressor protein class B from transposon Tn10) belongs to the Tet/Cam family of bacterial regulators and it controls the expression of Tn10 encoded tetracycline resistance. When tetracycline is not present, TetR recognises its operator sequence, tetO, for repressing tetA expression (resistance gene) and its own expression. Upon entry into the cell, tetracycline binds a pocket within the protein core of TetR and induces a conformational change leading to reorientation of DNA binding heads, which in turn abolishes operator binding for triggering resistance gene expression. TetR repressed tetracycline resistance element is responsible for the maintenance of antibiotic resistance traits in strains of Escherichia coli which confers an advantage in selection to E. coli expressing tetracycline resistance. Moreover, TetR has attracted widespread interest because regulators belonging to this family control the expression of genes which are involved in development, fatty acid metabolism, drug resistance and many other cellular processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional TetR Products

TetR NBP1-78424V

Research Areas for TetR Antibody (NBP1-78424V)

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