| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59085SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34432-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78424SS | |||
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0005.jpg "Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody [NBP2-15599] - A375 cells. Green: MOP3 protein stained by MOP3 antibody [N1N3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15599-img0003.jpg)
![Western Blot: BMAL1 Antibody [NBP2-15599] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP2-15599-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BMAL1 Antibody [NBP2-15599] - Treated 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] and PER2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15599_rabbit-polyclonal-bmal1-antibody-1610202419405778.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Western Blot: p14ARF Antibody [NB110-59085] - Detection of CDKN2A in HeLa whole cell extract.](http://images.novusbio.com/fullsize/p14ARF-Antibody-Western-Blot-NB110-59085-img0003.jpg "Western Blot: p14ARF Antibody [NB110-59085] - Detection of CDKN2A in HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: FoxP3 Antibody (SPM579) [NBP2-34385] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FoxP3 Antibody (SPM579).](http://images.novusbio.com/fullsize/FoxP3-Antibody-SPM579-Immunohistochemistry-Paraffin-NBP2-34385-img0002.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody (SPM579) [NBP2-34385] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FoxP3 Antibody (SPM579).")
![Western Blot: COBRA1 Antibody [NBP1-05977] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-05977 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. COBRA1 was also immunoprecipitated by NBP1-05976, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/COBRA1-Antibody-Western-Blot-NBP1-05977-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: COBRA1 Antibody [NBP1-05977] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-05977 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. COBRA1 was also immunoprecipitated by NBP1-05976, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - Human placenta. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/COBRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-05977-img0003.jpg)
![Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - human breast carcinoma. NBP1-05977 a dilution of 1:250.](http://images.novusbio.com/fullsize/COBRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-05977-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP2-27152-img0002.jpg "Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: TetR Antibody [NBP1-78424] - Analysis of TetR in 23kDa recombinant protein. Note the presence of a dimer.](http://images.novusbio.com/fullsize/TetR-Antibody-Western-Blot-NBP1-78424-img0003.jpg "Western Blot: TetR Antibody [NBP1-78424] - Analysis of TetR in 23kDa recombinant protein. Note the presence of a dimer.")
![Western Blot: PIMT Antibody [NBP2-44294] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44294 was used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by NB100-2595.](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP2-44294-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PIMT Antibody [NBP2-44294] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44294 was used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by NB100-2595.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: PIMT Antibody [NBP2-44294] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-NBP2-44294-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PIMT Antibody [NBP2-44294] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer.](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP2-44294-img0001.jpg)
![Western Blot: ETS1 associated protein II Antibody [NBP1-46178] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-TDP2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TDP2 was also immunoprecipitated by another rabbit anti-TDP2 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-Western-Blot-NBP1-46178-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ETS1 associated protein II Antibody [NBP1-46178] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-TDP2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TDP2 was also immunoprecipitated by another rabbit anti-TDP2 antibody, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: ETS1 associated protein II Antibody [NBP1-46178] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TDP2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ETS1-associated-protein-II-Antibody-Immunohistochemistry-NBP1-46178-img0004.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-71931-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HSF1 Antibody [NBP1-71931] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. Lot1 used at a dilution](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-NBP1-71931-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody NBP1-71931 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by two other rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunoprecipitation-NBP1-71931-img0003.jpg)