| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56097SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27175SS | |||
| Azide and BSA Free | NBP2-80564 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33138 | |||
| IHC-Prediluted | NBP2-44584 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45337 | |||
| Azide and BSA Free | NBP2-34716-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1920SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34401-0.1mg | |||
![Western Blot: Bax Antibody [NB100-56097] - Analysis of full-length recombinant mouse Bax using this antibody.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56097-img0003.jpg "Western Blot: Bax Antibody [NB100-56097] - Analysis of full-length recombinant mouse Bax using this antibody.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56097] - FFPE section of dog ischemic brain cortex stained for Bax expression using this antibody at 1:2000. At 2 hr post ischemia, Bax staining was seen in the dying neuron that had morphological features of apoptosis. In contrast, mophologically normal appearing neurons lacked Bax staining (arrowheads). Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56097-img0004.jpg)
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] - Representative images of IHF staining of EP2 or EP4 (green) and TRAP1 (NB300-555) (red) in subchondral bone 2 weeks post-sham or ACLT surgery (left) and quantitative analysis (right). The white arrows indicate TRAP-positive osteoclasts expressing EP2 or EP4. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-25520231539518.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Western-Blot-NB500-383-img0003.jpg "Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0002.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0009.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0001.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0004.jpg)
![Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cell-Surface-NB500-452-img0005.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (MEM-226) NB500-452PCP (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-226-Flow-Cytometry-NB500-452-img0007.jpg)
![Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Western-Blot-NBP2-27175-img0019.jpg "Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification]](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0018.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0014.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0015.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody .](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0016.jpg)
![Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-cellular spaces in cancerous areas of the sections. [40X magnification]](http://images.novusbio.com/fullsize/AG-2-Antibody-10E2-Immunohistochemistry-Paraffin-NBP2-27175-img0017.jpg)
![Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Western-Blot-NBP2-15189-img0003.jpg "Western Blot: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0010.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Formalin-paraffin human mantle cell lymphoma stained with Cyclin D1 Ab (Clone DCS-6). Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Immunohistochemistry-Paraffin-NBP2-15189-img0006.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-15189. Cyclin D1 staining of HCT116 cells using Alexa Fluor 647 conjugated Cyclin D1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0008.jpg)
![Flow Cytometry: Cyclin D1 Antibody (DCS-6) [NBP2-15189] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (DCS-6).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-DCS-6-Flow-Cytometry-NBP2-15189-img0009.jpg)
![Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Western-Blot-NBP2-32901-img0004.jpg "Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.")
![Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunocytochemistry-Immunofluorescence-NBP2-32901-img0007.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunohistochemistry-Paraffin-NBP2-32901-img0001.jpg)
![Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Flow-Cytometry-NBP2-32901-img0006.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0010.jpg "Western Blot: Nucleolin Antibody [NB100-1920] - Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Nucleolin antibody NB100-1920 (aNCL) used to probe western blot is indicated to the left of the image. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005121#sec002) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1920-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:750 conc. of Nucleolin antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed in normal cells and nuclear staining was observed in cancer cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Immunohistochemistry-Paraffin-NB100-1920-img0009.jpg)
![Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-Western-Blot-NB100-1920-img0006.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NBP2-13177-img0002.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.")
![Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Western-Blot-NBP2-34353-img0003.jpg "Western Blot: GFAP Antibody (SPM248) [NBP2-34353] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (SPM248).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM248) [NBP2-34353] - Formalin-fixed, paraffin-embedded human schwannoma stained with GFAP monoclonal antibody (SPM248).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Immunohistochemistry-Paraffin-NBP2-34353-img0001.jpg)
![Flow Cytometry: GFAP Antibody (SPM248) [NBP2-34353] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (SPM248) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM248-Flow-Cytometry-NBP2-34353-img0002.jpg)