| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97472-0.2mg | |||
| BSA Free | NBP1-97472-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97592-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12681-50ul | |||
![Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-71338-img0001.jpg "Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.")
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0006.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0002.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0003.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0004.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0005.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0007.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg "Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612216.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-3102024161227.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612226.jpg)
![Western Blot: AK3L1 Antibody [NBP2-97592] - Lane A: MCF-7 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:27 kDa](http://images.novusbio.com/fullsize/AK3L1-Antibody-Western-Blot-NBP2-97592-img0006.jpg "Western Blot: AK3L1 Antibody [NBP2-97592] - Lane A: MCF-7 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:27 kDa")
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-97592] - Staining of human AK3L1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-97592-img0005.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-97592] - Staining of human AK3L1 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-97592-img0004.jpg)
![Immunoprecipitation: AK3L1 Antibody [NBP2-97592] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-AK4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AK4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunoprecipitation-NBP2-97592-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AK3L1 Antibody [NBP2-97592] - Immunofluorescence staining of AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97592 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97592_rabbit-polyclonal-ak3l1-antibody-235202516144341.png)
![Western Blot: CD9 Antibody (EM-04) [NBP1-44876] - Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.](http://images.novusbio.com/fullsize/CD9-Antibody-EM-04-Western-Blot-NBP1-44876-img0001.jpg "Western Blot: CD9 Antibody (EM-04) [NBP1-44876] - Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.")
![Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) APC.](http://images.novusbio.com/fullsize/CD9-Antibody-EM-04-Flow-Cytometry-NBP1-44876-img0005.jpg)
![Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE](http://images.novusbio.com/fullsize/CD9-Antibody-EM-04-Flow-Cytometry-NBP1-44876-img0003.jpg)
![Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.](http://images.novusbio.com/fullsize/CD9-Antibody-EM-04-Flow-Cytometry-NBP1-44876-img0004.jpg)
![Western Blot: HMGB2 Antibody (JE52-82) [NBP3-32436] - Western blot analysis of HMGB2 on different lysates with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: K-562 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7:Mouse testis tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 24kDa Observed band size: 28 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32436) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-278202415242681.jpg "Western Blot: HMGB2 Antibody (JE52-82) [NBP3-32436] - Western blot analysis of HMGB2 on different lysates with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: K-562 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7:Mouse testis tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 24kDa Observed band size: 28 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32436) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HMGB2 Antibody (JE52-82) [NBP3-32436] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32436) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-298202414304166.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (JE52-82) [NBP3-32436] - Immunocytochemistry analysis of MCF7 cells labeling HMGB2 with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-29202410334041.jpg)
![Flow Cytometry: HMGB2 Antibody (JE52-82) [NBP3-32436] - Flow cytometric analysis of MCF7 cells labeling HMGB2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32436, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-29202416453289.jpg)
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)
![Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Western-Blot-NBP1-50529-img0001.jpg "Western Blot: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with HL60 cell line lysates (35ug/lane).")
![Immunocytochemistry/Immunofluorescence: LIN-28B Antibody [NBP1-50529] - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-50529-img0008.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0006.jpg)
![Flow Cytometry: LIN-28B Antibody [NBP1-50529] - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Flow-Cytometry-NBP1-50529-img0010.jpg)
![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP1-50529] - Analysis of anti-LIN28B antibody with human spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP1-50529-img0003.jpg)
![Western Blot: NDUFB11 Antibody [NBP3-12681] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:19 kDa](http://images.novusbio.com/fullsize/NDUFB11-Antibody-Western-Blot-NBP3-12681-img0001.jpg "Western Blot: NDUFB11 Antibody [NBP3-12681] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:19 kDa")
![Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP3-12681] - Staining of human NDUFB11 in human small intestine with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFB11-Antibody-Immunohistochemistry-Paraffin-NBP3-12681-img0003.jpg)
![Immunohistochemistry-Paraffin: NDUFB11 Antibody [NBP3-12681] - Staining of human NDUFB11 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFB11-Antibody-Immunohistochemistry-Paraffin-NBP3-12681-img0002.jpg)
![Immunoprecipitation: NDUFB11 Antibody [NBP3-12681] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NDUFB11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFB11 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size :19 kDa](http://images.novusbio.com/fullsize/NDUFB11-Antibody-Immunoprecipitation-NBP3-12681-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFB11 Antibody [NBP3-12681] - Staining of NDUFB11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12681 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12681_rabbit-polyclonal-ndufb11-antibody-235202511494028.png)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0006.jpg "Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.")
![Flow Cytometry: CD10 Antibody (MEM-78) [NB500-372]-Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/p_444_1.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC-CyTM7.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0002.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-CyTM5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0003.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0004.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0005.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)