| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-86682 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96990 | |||
| FITC | NBP1-96856 | |||
| Rhodamine | NBP1-96857 | |||
| Texas Red | NBP1-96985 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34816-0.1mg | |||
![Western Blot: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - A431(A) (0.003µg/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43548 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43548_mouse-catenin-alpha-1-mab-1g5-azide-and-bsa-free-western-blot-2220260322019.png "Western Blot: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - A431(A) (0.003µg/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43548 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/ Immunofluorescence: Catenin alpha 1 Antibody (1G5) - BSA Free [NBP3-43548] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on coverslips stained with NBP3-43548 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43548, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43548_mouse-catenin-alpha-1-mab-1g5-azide-and-bsa-free-immunocytochemistry-immunofluorescence-222026035115.png)
![Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23240_mouse-connexin-43-gja1-mab-m496-199202317594412.jpg "Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).")
![Western Blot: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - A431(A) (0.003ug/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43549 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43549_rabbit-catenin-alpha-1-mab-1g5-chimeric-azide-bsa-free-7420252226529.png "Western Blot: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - A431(A) (0.003ug/ml), HeLa(B) (0.03ug/ml) and MCF7(C) (0.01ug/ml) cell lysate. (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-43549 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Catenin alpha 1 Antibody (1G5) - Chimeric - BSA Free [NBP3-43549] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43549 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43549, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43549_rabbit-catenin-alpha-1-mab-1g5-chimeric-azide-bsa-free-74202522113031.png)
![Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18237-img0002.jpg "Western Blot: Tau Antibody [NBP3-18237] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18237] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237). Tissue: Kidney. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18237).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18237-img0001.jpg)
![Western Blot: sFRP-2 Antibody [NBP3-18244] - Western blot analysis of Mouse Brain showing detection of 33.5 kDa sFRP-2 protein using Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band(s): 37 kDa.](http://images.novusbio.com/fullsize/sFRP-2 Antibody-Western Blot-NBP3-18244-img0002.jpg "Western Blot: sFRP-2 Antibody [NBP3-18244] - Western blot analysis of Mouse Brain showing detection of 33.5 kDa sFRP-2 protein using Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band(s): 37 kDa.")
![Immunohistochemistry: sFRP-2 Antibody [NBP3-18244] - Immunohistochemistry analysis using Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244). Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/sFRP-2-Antibody-Immunohistochemistry-NBP3-18244-img0001.jpg)
![Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.](http://images.novusbio.com/fullsize/Tau Antibody-Western Blot-NBP3-18238-img0002.jpg "Western Blot: Tau Antibody [NBP3-18238] - Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.")
![Immunohistochemistry: Tau Antibody [NBP3-18238] - Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238). Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (NBP3-18238).](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NBP3-18238-img0001.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) - IHC-Prediluted [NBP2-48089] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).](http://images.novusbio.com/fullsize/c-Myc-Antibody-SPM237-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48089-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) - IHC-Prediluted [NBP2-48089] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).")
![Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.](http://images.novusbio.com/fullsize/Transferrin-Native-Protein-Dot-Blot-NBP1-97069-img0001.jpg "Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) - IHC-Prediluted [NBP2-44585] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-SPM587-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44585-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) - IHC-Prediluted [NBP2-44585] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).")
![Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0058.jpg "Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0057.jpg)
![Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB600-487-img0007.jpg "Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NB600-487] - Nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-487-img0005.jpg)