| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61795-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97472-0.2mg | |||
| BSA Free | NBP1-97472-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97761-50ul | |||
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0005.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Immunocytochemistry-Immunofluorescence-NBP2-61795-img0003.jpg)
![Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Flow-Cytometry-NBP2-61795-img0002.jpg)
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0004.jpg)
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0006.jpg)
![ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-ELISA-NBP2-61795-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0003.jpg "Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY CD38 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.")
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0006.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 2 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0001.jpg)
![Western Blot: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Analysis of cell extracts (35 ug) from 5 cell lines using anti-CD38 monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Western-Blot-NBP2-70376-img0002.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0004.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0005.jpg)
![Immunohistochemistry: CD38 Antibody (OTI1C9) - Azide and BSA Free [NBP2-70376] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-CD38 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-OTI1C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70376-img0007.jpg)
![Western Blot: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of extracts (35ug) from 3 different cell lines and 5 different tissue lysates by using anti-SFRP2 monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Western-Blot-NBP2-74151-img0006.jpg "Western Blot: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of extracts (35ug) from 3 different cell lines and 5 different tissue lysates by using anti-SFRP2 monoclonal antibody (1:500).")
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0005.jpg)
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0001.jpg)
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0002.jpg)
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0003.jpg)
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0004.jpg)
![Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-70383-img0001.jpg "Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.")
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0006.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0002.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0003.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0004.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0005.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0007.jpg)
![Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-71338-img0001.jpg "Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.")
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0006.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0002.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0003.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0004.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0005.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0007.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg "Western Blot: Transferrin Antibody [NBP1-97472] - Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487) licensed under a CC-BY license.")
![Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NBP1-97472-img0001.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612216.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-3102024161227.jpg)
![Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97472_rabbit-polyclonal-transferrin-antibody-31020241612226.jpg)
![Western Blot: FARSLA Antibody [NBP2-97761] - Anti-FARSLA rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa](http://images.novusbio.com/fullsize/FARSLA-Antibody-Western-Blot-NBP2-97761-img0006.jpg "Western Blot: FARSLA Antibody [NBP2-97761] - Anti-FARSLA rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa")
![Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunohistochemistry-Paraffin-NBP2-97761-img0005.jpg)
![Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunohistochemistry-Paraffin-NBP2-97761-img0004.jpg)
![Immunoprecipitation: FARSLA Antibody [NBP2-97761] - FARSLA was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-FARSLA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FARSLA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunoprecipitation-NBP2-97761-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FARSLA Antibody [NBP2-97761] - Immunofluorescence staining of FARSLA in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97761 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97761_rabbit-polyclonal-farsla-antibody-235202516335033.png)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0006.jpg "Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.")
![Flow Cytometry: CD10 Antibody (MEM-78) [NB500-372]-Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/p_444_1.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC-CyTM7.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0002.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-CyTM5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0003.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0004.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0005.jpg)
![Western Blot: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-27820241631643.jpg "Western Blot: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29820241435273.jpg)
![Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunocytochemistry analysis of HepG2 cells labeling integrin beta 4 binding protein with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29202410234218.jpg)
![Flow Cytometry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Flow cytometric analysis of HepG2 cells labeling integrin beta 4 binding protein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32303, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29202416453222.jpg)