| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02952-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35163-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35468-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56140SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83431-25ul | |||
![Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2-Antibody-Western-Blot-NBP3-02952-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2 Antibody-Western Blot-NBP3-02952-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519302962.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519294585.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411353099.jpg "Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Western blot analysis of various lysates using Protein Phosphatase 1 beta Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411364695.jpg)
![Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunofluorescence analysis of U2OS cells using Protein Phosphatase 1 beta Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411392924.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411353020.jpg)
![Immunohistochemistry: Protein Phosphatase 1 beta Antibody [NBP3-35163] - Immunohistochemistry analysis of paraffin-embedded Human liver using Protein Phosphatase 1 beta Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35163_rabbit-protein-phosphatase-1-beta-pab-1012202411364636.jpg)
![Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35468_rabbit-ptprn2-pab-912202415161857.jpg "Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35468_rabbit-ptprn2-pab-912202415175217.jpg)
![Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35468_rabbit-ptprn2-pab-912202415121364.jpg)
![Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35468_rabbit-ptprn2-pab-912202415111847.jpg)
![Western Blot: PTPRN2 Antibody [NBP3-35468] - Western blot analysis of various lysates using PTPRN2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35468_rabbit-ptprn2-pab-912202414112864.jpg)
![Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]](http://images.novusbio.com/fullsize/DARPP-32-[p-Thr34]-Antibody-Western-Blot-NB300-224-img0001.jpg "Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]")
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455812.jpg "Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-13112023114762.jpg)
![Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455813.jpg)
![Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455817.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp2-75097_goat-polyclonal-myosin-phosphatase-antibody-1311202311455810.jpg)
![Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69989-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0005.jpg)
![Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Flow-Cytometry-NBP2-69989-img0002.jpg)
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0003.jpg)
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0004.jpg)
![Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target Name: G6PC3. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0005.jpg "Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target Name: G6PC3. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml")
![Western Blot: G6PC3 Antibody [NBP2-82857] - WB Suggested Anti-G6PC3 Antibody. Titration: 1.0 ug/ml. Positive Control: 721_B Whole CellG6PC3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0001.jpg)
![Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target Name: G6PC3. Sample Type: Hela. Antibody Dilution: 1.0ug/mlG6PC3 is strongly supported by BioGPS gene expression data to be expressed in HeLa](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0002.jpg)
![Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target Name: G6PC3. Sample Type: Human Fetal Brain. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0003.jpg)
![Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target Name: G6PC3. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0004.jpg)
![Western Blot: G6PC3 Antibody [NBP2-82857] - Host: Rabbit. Target: G6PC3. Positive control (+): MCF7 (N10). Negative control (-): Human Placenta (PL). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/G6PC3-Antibody-Western-Blot-NBP2-82857-img0006.jpg)
![Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56140] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:1000 conc. of PTPN13 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PTPN13 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/PTPN13-PTPL1-Antibody-Immunohistochemistry-Paraffin-NB100-56140-img0001.jpg "Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56140] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:1000 conc. of PTPN13 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of PTPN13 was observed. Staining was performed by Histowiz.")
![Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-Antibody-Western-Blot-NB110-38889-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody NB110-38889 (lot NB110-38889-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot NB110-38889-1) of this antibody and by another rabbit anti-MYPT1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0010.jpg "Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunocytochemistry-Immunofluorescence-H00000053-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Immunohistochemistry-Paraffin-H00000053-M01-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi . Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-Western-Blot-H00000053-M01-img0004.jpg)
![ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01] - Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACP2-Antibody-M1-4A12-ELISA-H00000053-M01-img0009.jpg)
![Western Blot: MDP1 Antibody [NBP1-83431] - Analysis in human cell lines MCF-7 and HeLa using anti-MDP1 antibody. Corresponding MDP1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/MDP1-Antibody-Western-Blot-NBP1-83431-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: MDP1 Antibody [NBP1-83431] - Analysis in human cell lines MCF-7 and HeLa using anti-MDP1 antibody. Corresponding MDP1 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MDP1 Antibody [NBP1-83431] - Staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MDP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83431-img0005.jpg)
