| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83668-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90103-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43349-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2079SS | |||
| Azide and BSA Free | NBP2-80732 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22128SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB91871-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82685-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90162-25ul | |||
![Immunocytochemistry/Immunofluorescence: SNRK Antibody [NBP1-83668] - Analysis of human brain microvascular endothelial cells using SNRK antibody. SNRK overexpression is found to be highly expressed in the nucleus, SNRK RED, DAPI BLUE. Image from verified customer review.](http://images.novusbio.com/fullsize/SNRK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83668-img0012.jpg "Immunocytochemistry/Immunofluorescence: SNRK Antibody [NBP1-83668] - Analysis of human brain microvascular endothelial cells using SNRK antibody. SNRK overexpression is found to be highly expressed in the nucleus, SNRK RED, DAPI BLUE. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: SNRK Antibody [NBP1-83668] - Staining of human cell line U-2 OS shows localization to cell junctions & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SNRK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83668-img0010.jpg)
![Immunohistochemistry-Paraffin: SNRK Antibody [NBP1-83668] -Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and endocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83668_rabbit-polyclonal-snrk-antibody-immunohistochemistry-paraffin-1102024105527..jpg)
![Immunohistochemistry-Paraffin: SNRK Antibody [NBP1-83668] -Staining of human lymphoid tissues shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83668_rabbit-polyclonal-snrk-antibody-immunohistochemistry-paraffin-1102024105616..jpg)
![Immunohistochemistry-Paraffin: SNRK Antibody [NBP1-83668] -Staining of human testis shows strong nucleus positivity in endothelial cells, leydig cells and moderate nucleus positivity in cells in seminiferous cells.](http://images.novusbio.com/fullsize/antibody/nbp1-83668_rabbit-polyclonal-snrk-antibody-immunohistochemistry-paraffin-110202410575..jpg)
![Immunohistochemistry-Paraffin: SNRK Antibody [NBP1-83668] -Staining of human skeletal muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-83668_rabbit-polyclonal-snrk-antibody-immunohistochemistry-paraffin-1102024105758..jpg)
![Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-90103-img0007.jpg "Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90103-img0009.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0014.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0011.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0012.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0013.jpg)
![Western Blot: ULK2 Antibody [NBP1-33136] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ULK2-Antibody-Western-Blot-NBP1-33136-img0014.jpg "Western Blot: ULK2 Antibody [NBP1-33136] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ULK2 Antibody [NBP1-33136] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ULK2 stained by ULK2 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/ULK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33136-img0012.jpg)
![Immunohistochemistry-Paraffin: ULK2 Antibody [NBP1-33136] - MCF7 xenograft, using ULK2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ULK2-Antibody-Immunohistochemistry-Paraffin-NBP1-33136-img0004.jpg)
![Western Blot: ULK2 Antibody [NBP1-33136] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term at a dilution of 1:1000.](http://images.novusbio.com/fullsize/ULK2-Antibody-Western-Blot-NBP1-33136-img0008.jpg)
![Western Blot: ULK2 Antibody [NBP1-33136] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ULK2-Antibody-Western-Blot-NBP1-33136-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ULK2 Antibody [NBP1-33136] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ULK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33136-img0003.jpg)
![Immunohistochemistry-Paraffin: ULK2 Antibody [NBP1-33136] -Rat brain.ULK2 stained by ULK2 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-33136_rabbit-polyclonal-ulk2-antibody-immunohistochemistry-paraffin-562024124112..jpg)
![Western Blot: ULK2 Antibody [NBP1-33136] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (NBP1-33136) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33136_rabbit-polyclonal-ulk2-antibody-91020242012201.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ULK2 Antibody [NBP1-33136] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33136_rabbit-polyclonal-ulk2-antibody-1610202419405726.jpg)
![Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0004.jpg "Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.")
![Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0001.jpg)
![Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0003.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0003.jpg "Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.")
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0006.jpg)
![Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Flow-Cytometry-NBP2-37575-img0004.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0002.jpg)
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human lung cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0005.jpg)
![ELISA: IRAK4 Antibody (2H9) [NBP2-37575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-ELISA-NBP2-37575-img0001.jpg)
![Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Western-Blot-NB100-2079-img0004.jpg "Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Immunohistochemistry-Paraffin-NB100-2079-img0003.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0006.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0005.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0006.jpg "Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.")
![Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-22128-img0003.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0005.jpg)
![Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0002.jpg)
![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0004.jpg)
![ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-3H6-ELISA-NBP2-22128-img0001.jpg)
![Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in thymocytes.](http://images.novusbio.com/fullsize/MLKL-p-Thr357-Antibody-954724-Immunohistochemistry-Paraffin-MAB91871-img0002.jpg "Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in thymocytes.")
![Immunohistochemistry: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/MLKL-p-Thr357-Antibody-954724-Immunohistochemistry-MAB91871-img0001.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Truncated Sin1/MAPKAP1 displaces endogenous Sin1/MAPKAP1 from mTORC2 in DLD1 colon cancer cells. Sin1/MAPKAP1 constructs incorporate into mTORC2 and displace endogenous Sin1/MAPKAP1. Constructs were induced for 72 hours prior to immune precipitation. mTORC2 subunits, mTOR and Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-deltaSin1/MAPKAP1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor and mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/20086), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0010.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Truncated Sin1/MAPKAP1 displaces endogenous Sin1/MAPKAP1 from mTORC2 in DLD1 colon cancer cells. Sin1/MAPKAP1 constructs incorporate into mTORC2 and displace endogenous Sin1/MAPKAP1. Constructs were induced for 72 hours prior to immune precipitation. mTORC2 subunits, mTOR and Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-deltaSin1/MAPKAP1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor and mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/20086), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NB110-40424-img0009.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0008.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-658] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954 whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-658) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0025.jpg "Western Blot: DNA-PKcs Antibody [NB100-658] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954 whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-658) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-658] - Detection of Human DNA-PKcs in HeLa whole cell lystate using NB100-658. DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-659 and NB100-660.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0023.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-658] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. DNA-PKcs antibody at 1:1000 dilution O/N. Secondary antibody: HRP-conjugated Donkey anti-rabbit IgG polyclonal antibody at 1:2000 dilution. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Western-Blot-NB100-658-img0024.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DNA-PKcs Antibody [NB100-658] - Molecular Characterization of G1 Resection. End joining events in GC92 cells treated with siKu70/80, siLig4, siLig1/3, or DNA-PKcs antibody. Data are mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5316416/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNA-PKcs-Antibody-Knockdown-Validated-NB100-658-img0026.jpg)
![Western Blot: DNA-PKcs Antibody [NB100-658] - Molecular Characterization of G1 Resection(A) Schematic of the NHEJ reporter assay. The repair of two I-SceI-induced DSBs can result in loss of the intervening fragment, which is detected by a CD4+ signal (Rass et al., 2009). CD4+ clones were amplified by PCR (green arrows) across the repair site & sequenced. Repair of the two DSBs can also occur without loss of the intervening fragment, which escapes detection.(B) gamma H2AX foci in GC92 WT & Artemis KO cells treated with siDNA-PKcs or siCtIP. Cells were transfected with I-SceI, & foci were scored in I-SceI+ & I-SceI− cells (identified by immunofluorescence [IF] against I-SceI). Data are mean ± SEM.(C) End joining events in GC92 WT & Artemis KO cells containing the NHEJ reporter substrate. Cells were transfected with RFP or cMyc-Artemis constructs. Events were quantified by the fraction of CD4+ & RFP/cMyc+cells relative to all RFP/cMyc+cells, & results were normalized to WT cells. Data are mean ± SEM.(D) End joining events in GC92 WT & Artemis KO cells treated with siCtIP. Data are mean ± SEM.(E) Distribution of deletion sizes obtained from the sequence analysis of GC92 WT & siLig1/3-treated cells. nt, nucleotide.(F) End joining events in GC92 cells treated with siKu70/80, siLig4, siLig1/3, or siDNA-PKcs. Data are mean ± SEM.See also Table S1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28132842), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-658_rabbit-polyclonal-dna-pkcs-antibody-31020241682113.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-82685] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82685-img0009.jpg)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunohistochemistry-Paraffin-NBP1-82685-img0002.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0007.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0008.jpg)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-422025132224..png)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-422025132315..png)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-42202513248..png)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Analysis in human esophagus and cerebral cortex tissues. Corresponding ANXA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Analysis in human esophagus and cerebral cortex tissues. Corresponding ANXA1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon, esophagus, lung and lymph node using Anti-Annexin A1 antibody NBP1-90162 (A) shows similar protein distribution across tissues to independent antibody NBP1-90161 (B).](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANXA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-90162-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis using Anti-ANXA1 antibody NBP1-90162 (A) shows similar pattern to independent antibody NBP1-90161 (B).](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-90162-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody [NBP1-90162] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90162-img0011.jpg)
![Western Blot: Annexin A1 Antibody [NBP1-90162] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Western-Blot-NBP1-90162-img0007.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human lung shows strong positivity in macrophages.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0020.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human lymph node shows moderate to strong positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0021.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human esophagus shows moderate to strong positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0022.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0024.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0025.jpg)