| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84750-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44073 | |||
| BSA Free | NBP2-34487-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81272-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76800SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37262-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61852-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-15185SS | |||
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0011.jpg "Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.")
![Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining shown in green.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84750-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human testis shows moderate to cytoplasmic positivity in spermatogonia and Leydig cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0009.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression & metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta 1 (a) or beta 4 (b) receptor (green) & FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 μm. (c) MS imaging of cross sections of the medium-conditioned blank (upper panels) & MM231 cell culture-laden (bottom panels) hydrogel scaffolds. (d) Examples of ROC (upper) & box-and-whiskers (bottom) plots from the MS imaging analysis, showing data for a compound with m/z 663.024, where differences between the hydrogel samples cultured with or without MM231 cells are clearly evident. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30257480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84750_rabbit-polyclonal-fak-antibody-310202415284542.jpg)
![Western Blot: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Western-Blot-NBP2-34487-img0006.jpg "Western Blot: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Immunohistochemistry-Paraffin-NBP2-34487-img0005.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Staining of human small intestine shows strong immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Immunohistochemistry-Paraffin-NBP2-34487-img0001.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Staining of human tonsil shows strong positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Immunohistochemistry-Paraffin-NBP2-34487-img0002.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Staining of human colon shows strong immunoreactivity in some lymphoid cells.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Immunohistochemistry-Paraffin-NBP2-34487-img0003.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1667) [NBP2-34487] - Staining of human cerebellum shows moderate positivity in the molecular layer.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-CL1667-Immunohistochemistry-Paraffin-NBP2-34487-img0004.jpg)
![Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/MAP3K13-Antibody-Western-Blot-NBP1-81272-img0009.jpg "Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.")
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human placenta shows strong membranous positivity in trphoblastic cells.](http://images.novusbio.com/fullsize/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0013.jpg)
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0010.jpg)
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0011.jpg)
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0012.jpg)
![Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Induced Expression Validation in Mouse Cell Line. Loading: 15 ug of RAW 264.7 cell lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Cells were treated with LPS (0.3 ug/ml) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).](http://images.novusbio.com/fullsize/IKK-epsilon-IKBKE-Antibody-Western-Blot-NBP1-76800-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Induced Expression Validation in Mouse Cell Line. Loading: 15 ug of RAW 264.7 cell lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Cells were treated with LPS (0.3 ug/ml) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).")
![<b>Biological Strategies Validation. </b>Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IKK-epsilon-IKBKE-Antibody-Western-Blot-NBP1-76800-img0007.jpg)
![Western Blot: IKK epsilon/IKBKE Antibody - BSA Free [NBP1-76800] - Validation in Jurkat Cell Lysate. Antibodies: IKK epsilon/IKBKE (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76800_rabbit-polyclonal-ikk-epsilon-ikbke-antibody-271120242074671.jpg)
![Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IKK-epsilon-IKBKE-Antibody-Western-Blot-NBP1-76800-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody - BSA Free [NBP1-76800] - Validation of IKK epsilon in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76800_rabbit-polyclonal-ikk-epsilon-ikbke-antibody-2711202419451291.gif)
![Immunohistochemistry: IKK epsilon/IKBKE Antibody - BSA Free [NBP1-76800] - Validation of IKK epsilon in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76800_rabbit-polyclonal-ikk-epsilon-ikbke-antibody-2711202419201585.jpg)
![Western Blot: Casein Kinase 1 delta Antibody [NB100-2919] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. Antibody at 0.2 ug/mL.](http://images.novusbio.com/fullsize/Casein-Kinase-1-delta-Antibody-Western-Blot-NB100-2919-img0001.jpg "Western Blot: Casein Kinase 1 delta Antibody [NB100-2919] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. Antibody at 0.2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 delta Antibody [NB100-2919] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Casein-Kinase-1-delta-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2919-img0002.jpg)
![ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-244202310354.jpg "ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-western-blot-2442023103658.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517344223.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-52202517311071.jpg)
![Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15776_rabbit-erk1-mab-0b0c1-5220251733559.jpg)
![Western Blot: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Western blot analysis using CAMK2G mouse mAb against PC-12 (1), Jurkat (2), T47D (3), HepG2 (4) cell lysate.](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Western-Blot-NBP2-37262-img0004.jpg "Western Blot: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Western blot analysis using CAMK2G mouse mAb against PC-12 (1), Jurkat (2), T47D (3), HepG2 (4) cell lysate.")
![Immunohistochemistry-Paraffin: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CAMK2G mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Immunohistochemistry-Paraffin-NBP2-37262-img0009.jpg)
![Flow Cytometry: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Flow cytometric analysis of Jurkat cells using CAMK2G mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Flow-Cytometry-NBP2-37262-img0005.jpg)
![Western Blot: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Western blot analysis using CAMK2G mAb against human CAMK2G (AA: 322-481) recombinant protein. (Expected MW is 44 kDa)](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Western-Blot-NBP2-37262-img0002.jpg)
![Western Blot: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Western blot analysis using CAMK2G mAb against HEK293 (1) and CAMK2G (AA: 322-481)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Western-Blot-NBP2-37262-img0003.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CAMK2G mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-Immunohistochemistry-Paraffin-NBP2-37262-img0008.jpg)
![ELISA: CaM Kinase II gamma Antibody (8G10C1) [NBP2-37262] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CaM-Kinase-II-gamma-Antibody-8G10C1-ELISA-NBP2-37262-img0001.jpg)
![Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-Western-Blot-NBP2-37448-img0003.jpg "Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.")
![Immunohistochemistry-Paraffin: ILK Antibody (3A9) [NBP2-37448] - Analysis of rectum cancer tissues using ILK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-Immunohistochemistry-NBP2-37448-img0006.jpg)
![Flow Cytometry: ILK Antibody (3A9) [NBP2-37448] - Analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-Flow-Cytometry-NBP2-37448-img0004.jpg)
![Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa)](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-Western-Blot-NBP2-37448-img0002.jpg)
![Immunohistochemistry-Paraffin: ILK Antibody (3A9) [NBP2-37448] - Analysis of esophageal cancer tissues using ILK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-Immunohistochemistry-NBP2-37448-img0005.jpg)
![ELISA: ILK Antibody (3A9) [NBP2-37448] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ILK-Antibody-3A9-ELISA-NBP2-37448-img0001.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0006.jpg "Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Immunohistochemistry-NBP2-61852-img0004.jpg)
![Flow Cytometry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Flow-Cytometry-NBP2-61852-img0002.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mAb against human *** (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0005.jpg)
![Western Blot: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Western-Blot-NBP2-61852-img0007.jpg)
![Immunohistochemistry: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-Immunohistochemistry-NBP2-61852-img0003.jpg)
![ELISA: AMPK alpha 2 Antibody (8E11H5) [NBP2-61852] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/AMPK-alpha-2-Antibody-8E11H5-ELISA-NBP2-61852-img0001.jpg)
![Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr142-Antibody-EM-54-Western-Blot-NBP2-37708-img0001.jpg "Western Blot: CD3 zeta [p Tyr142] Antibody (EM-54) [NBP2-37708] - Analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518274621.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518431912.jpg)
![Immunocytochemistry/Immunofluorescence: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in neuro2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/TrkC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15185-img0001.jpg "Immunocytochemistry/Immunofluorescence: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in neuro2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: TrkC Antibody [NBP2-15185] - TrkC antibody was tested in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TrkC-Antibody-Immunohistochemistry-NBP2-15185-img0003.jpg)